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Method for preparing corn protein peptide with high F valve by microorganism zymotechnics

A microbial fermentation method and corn protein peptide technology, applied in the direction of fermentation, etc., can solve the problems of difficult enzymatic hydrolysis and low product yield, and achieve the effect of reducing hydrophobicity and increasing yield

Inactive Publication Date: 2008-02-20
黑龙江省宏达生物工程有限公司
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Problems solved by technology

The results of the novelty search show that the direct hydrolysis method is used to produce high F value oligopeptides from corn gluten powder, but corn gluten has a special odor, dark yellow color, compact structure, and strong hydrophobicity, so it is not easy to enzymatically hydrolyze carried out, therefore, the product yield is lower
At present, there are no relevant reports on the industrial production of high F value oligopeptides at home and abroad

Method used

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  • Method for preparing corn protein peptide with high F valve by microorganism zymotechnics

Examples

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Embodiment Construction

[0022] The present invention will be further described below in conjunction with accompanying drawing and specific embodiment:

[0023] 1.1. Source of fermentation strain

[0024] In this example, Aspergillus niger and Bacillus natto were purchased from China Industrial Microorganism Culture Collection and Management Center, and the strain number Aspergillus niger CICC2377 was used to produce acid protease; Bacillus natto CICC20443 was used to digest and enzymatically hydrolyze zein. Aromatic amino acids.

[0025] 1.2 Strain mutagenesis

[0026] In conjunction with Figure 2, the Aspergillus niger CICC2377 selected in this example is a fungus specialized in the production of acid protease. The fermentation broth can directly act on the corn gluten slurry to produce corn protein peptides and various amino acids, which contain a certain amount of aromatic amino acids. Bacillus natto CICC20443 can produce high F-value zein peptides after eliminating most of the aromatic amino acid...

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Abstract

The invention provides a method of preparing corn pertain peptide with high F value via microorganism fermentation. Expanded maize yellow powder that is taken as the raw material of the method is added with water by a weight ratio of 1:10 and is superfine-comminuted with a colloid grinder, sterilized with high temperature, cooled and inoculated with 8 -10 percent of aspergillus niger fermentation liquid, hydrolyzed with a temperature of 40-50 DEG C for 20- 24 hours, cooled to 35 DEG C after sterilization, inoculated with bacillus natto suspension by a volume ratio of 3-5 percent, fermented at an insulated temperature for 18-20 hours, sterilized with high temperature, centrifugal pulp cleaned and decolorized, collecting the supernatant fluid, which is condensed and spray-dried, thereby acquiring fine powder of corn pertain peptide with high F value. Pre-processing techniques such as squeezing expansion and superfine-comminution are adopted in the invention to open of the tight structure of corn pertain, facilitate enzymolysis and greatly improve the yield of the product.

Description

(1) Technical field [0001] The invention relates to the technical field of biological preparation, in particular to a method for preparing high F-value zein peptide. (2) Background technology [0002] Corn is one of the three major food crops in the world, and it is also an important feed raw material. Because of its high per unit yield and great potential for increasing production, it occupies an important position in agricultural production. Zein can be used to extract excellent products such as zein, zeaxanthin, and glutamic acid, and can also prepare corn active peptides with various physiological functions, such as zein peptides, high F value oligopeptides, blood pressure-lowering peptides, etc. , thus greatly increasing the added value of corn processing. Although corn is used in a wide range, for a long time, people have mainly used corn to produce starch, and the waste of corn protein resources is very large. For this reason, some developed countries in the world a...

Claims

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Application Information

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IPC IPC(8): C12P21/00A23J1/00
Inventor 陈成谢吉望杨晓宏
Owner 黑龙江省宏达生物工程有限公司
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