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Heavy ion radiation induced chromosome fracture calculation method

A technology of heavy ion radiation and calculation method, which is applied in the field of calculation of chromosome breakage induced by heavy ion radiation, can solve the problems that there is no theoretical calculation method, and the inevitable relationship between the radiation sensitivity of chromosome damaged cells is not clearly stated.

Inactive Publication Date: 2008-02-20
INST OF MODERN PHYSICS CHINESE ACADEMY OF SCI
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  • Description
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  • Application Information

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Problems solved by technology

However, this method is only applied to the study of radiation-induced chromosomal damage, without clearly proposing the necessary relationship between chromosomal damage and cell radiation sensitivity, and there is no report on theoretical calculation methods

Method used

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  • Heavy ion radiation induced chromosome fracture calculation method
  • Heavy ion radiation induced chromosome fracture calculation method
  • Heavy ion radiation induced chromosome fracture calculation method

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Effect test

Embodiment 1

[0030] Embodiment 1: A method for calculating chromosome breakage induced by heavy ion radiation, including:

[0031] Step 1: Cultivate the test cells, and perform the following operations when they are in the exponential growth phase;

[0032] Human normal liver cell line L02 (purchased from CCTCC) was incubated with RPMI-1640 medium containing 10% fetal bovine serum at 37°C, 5% CO 2 cultured in a constant temperature incubator, and 0.25 U / ml of insulin was added to the culture medium. Cell concentration was 5 x 10 6 / ml.

[0033] Step 2: Determining the surface area of ​​the cells in the flat state in the culture flask;

[0034] Inoculate 2ml of L02 cell suspension that was well mixed in a φ35mm disposable culture dish.

[0035] Step 3: Determination of G using flow cytometry 2 The content of phase cells;

[0036] Irradiation is performed when one doubling of it occurs.

[0037] Step 4: Determining the average LET of the rays used to irradiate the cell;

[0038] The ...

Embodiment 2

[0049] Embodiment 2: A method for calculating chromosome breakage induced by heavy ion radiation, the actual number of broken cells is calculated as:

[0050] N ′ = F × π φ 2 4 Numbe r cells × K 2 K 1 K 3 - - - ( 4 )

[0051] K 1 = Σ 1 n de / dx n ...

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Abstract

The utility model relates to a calculation method of chromosome breakage induced by heavy ion radiation, in particular to a number calculation method of broken cell of the chromosome after the cell accepts the irradiation of heavy ion. The calculation method of chromosome breakage induced by heavy ion radiation is characterized in that: A. The subject cell is cultured and the following operation is made when the subject cell is in exponential phase for a long term; B. Surface area of the cell is tested when the cell is at the spreading status inside the culture bottle; C. Cell content of G2 period is tested using a flow cytometer; D. Average LET of radiation ray is adopted when the cell is irradiated and tested. E. Number N of the irradiated cell is tested; F. Inner bottom area is tested for the cell culture bottle; G. The above testing value is brought into equation for solving. The utility model has the advantages that the establishment belonging to biological technology field of chromosome breakage theory calculation model occurring inside the cell after the tumor cells accepts the irradiation of the heavy ion can solve the predicted bottleneck for radiosensitivity of the tumor cells when heavy ion clinical radiotherapy plan is made and the application of the utility model also can quickly and accurately test radiation sensibility of tumor cells upon the heavy ion radiation for the tumor cells under the offline condition.

Description

Technical field: [0001] The invention mainly relates to a calculation method for chromosome breakage induced by heavy ion radiation, in particular to a calculation method for the number of cells with chromosome breakage after cells are irradiated by heavy ions. Background technique: [0002] Heavy ions are currently recognized as the most effective particles in the field of radiation therapy, because they have significantly better biophysical properties than other common rays such as X-rays (Kraft G.Tumor therapy with heavy charged particles.Progress in particle and nuclear Physics, 2000, 45: s473-544). Such as high relative biological effect, low oxygen ratio, low repair effect, low side scatter, etc. It is generally believed that the radiosensitivity of biological materials to different rays determines the difference in biological endpoints caused by radiation. [0003] It has been reported abroad that the precocious chromosome agglutination technique is used to study th...

Claims

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Application Information

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IPC IPC(8): G01N15/00C12Q1/02G06F19/00G06F19/18
Inventor 杨建设李文建
Owner INST OF MODERN PHYSICS CHINESE ACADEMY OF SCI
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