Method and device for producing biological siliceous material with reactor culture sponge or its cell

A reactor, bio-silicon technology, applied in biochemical equipment and methods, microorganisms, tissue culture, etc., can solve problems such as waste of resources, and achieve the effect of easy collection and low substrate concentration

Inactive Publication Date: 2011-06-08
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At the same time, sponges are also important drug source organisms, and sponge reactor culture or sponge cell culture will be the most likely way to solve the biomass source in the future, but the siliceous material in the sponge accounts for more than 50% of the dry weight of the sponge , most of them are treated as processing waste at present, resulting in a great waste of resources

Method used

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  • Method and device for producing biological siliceous material with reactor culture sponge or its cell
  • Method and device for producing biological siliceous material with reactor culture sponge or its cell
  • Method and device for producing biological siliceous material with reactor culture sponge or its cell

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Experimental program
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Effect test

Embodiment 1

[0045] Wash the freshly collected luxuriant membrane sponge with 200mL of natural seawater filtered through a 0.22μM filter membrane to remove algae and sediment attached to the surface, and store it in the laboratory for three days; cut the sponge with a scalpel into 0.5×0.5 cm 2 Small pieces, ten pieces in total, about 1 gram, transferred into 200mL natural seawater which was sterilized by 0.22μm membrane filter and added with 400mg / L gentamycin, and allowed to stand for 10 minutes; in a liquid-liter plug-in reactor Add 1.8L of natural seawater filtered and sterilized by a 0.22μm filter membrane; insert the treated sponge block on the glass base of the liquid lift plug-in reactor, and cultivate in the culture reactor; the final concentration of adding 200,000 per week The golden algae of cell / mL is used as food; the sponge is taken out together with the plug every week, the surface dirt is removed and the surface seawater is dried, and weighed. The sponge tissue showed an i...

Embodiment 2

[0047] Take the temporary laboratory sponge and cut it into 0.5×0.5cm with a scalpel 2 Small pieces, ten pieces in total, 1.3g, transferred into 200mL natural seawater which was sterilized by filtration and added with 400mg / L gentamycin, and allowed to stand for 10 minutes; The natural seawater sterilized by μm filter membrane; the treated sponge block was inserted on the glass base of the liquid-lift plug reactor, and cultivated in the culture reactor; gold with a final concentration of 200,000 cells / mL was added every week Algae were used as food, and 60 μm silane coupling agent 3-ureidopropyltrimethoxysilane was added at the same time; the bones discarded by the sponge were collected when weighing every week, and dried; after the sixth week, the sponge tissue was collected and weighed. Weighing about 1.2g; all the spongy tissue and bone residues were treated with 20mL of mixed acid of concentrated sulfuric acid: concentrated nitric acid=3:1 (volume ratio) at 80°C for one ho...

Embodiment 3

[0049] After the sponge was collected, it was treated with 7ppm CuSO4 and 400mg / L GentamycinSulfate (Justaware Pharmaceutical Co.Ltd) for three hours after 24 hours of temporary cultivation. Refer to the literature (Primmorphs from archaeocytes-dominant cell population of the sponge Hymeniacidon perleve: Improved cell proliferation and spiculogenesis (Biotechnology and Bioengineering, v84-5, 583-590)) to perform Ficoll cell density gradient separation, and take 12-15% of the cells for ADCP culture. Cell culture was carried out in 9 cm Petri dishes (Corning) at a seeding density of 5 × 10 6 cell / mL. The culture medium is natural seawater filtered through 0.22 μm, and 60 μM silane coupling agent 3-ureidopropyltrimethoxysilane and 30 μM ferric citrate are added. Incubate at 18°C ​​in the dark, and replace 100% of the medium every two days. The presence of new spicules can be found in the cell aggregates after culture, see Figure 5 . Arrows indicate new spicules.

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Abstract

The invention relates to a method and a device of reactor culture sponge or the cell production biological siliceous material. The reactor applies natural seawater, micro algae culture sponge of the normal sponge bait, wherein the adhesion base of the growth face which forms 45-135 degree with the horizontal direction, the sponge or the cell can grow along the growth surface, the natural seawaterhas silane coupling agent, the bone spicule is acquired with the phenomenon that the original skeleton is abandoned in the growth and motion process of the sponge structure, or the biological siliceous material is acquired by producing bone spicule in the exsomatize sponge cell culture system. The production method introduces silane coupling agent by constructing the sponge culture reactor, whichproceeds with the biological synthesis of the siliceous material with the chemical modification in order to make the sponge or the sponge cell into 'factory' of specific biological siliceous material, and provides the probability of the application for synthesizing the sponge siliceous bone spicule.

Description

technical field [0001] The invention relates to a method for obtaining biological siliceous materials by using biological methods, in particular to a method and a device for producing biological siliceous materials by cultivating sponges or cells thereof in a reactor. Background technique [0002] Sponges are the lowest multicellular animals and have the ability to synthesize their own skeletons using low concentrations of orthosilicic acid in water. Compared with conventional methods, this biosynthetic process is carried out in a natural environment with extremely low substrate concentrations. The basic unit of the sponge skeleton is the spicule, and its chemical composition is silicon dioxide ((SiO 2 ) 2~5 ·H 2 O), consistent with the chemical composition of commercial optical glass, at the same time, studies have shown that this biological siliceous material has good biocompatibility and chemical stability, and its material properties such as fracture strength are supe...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/06C12N5/07
Inventor 张卫曹旭鹏虞星炬金美芳
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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