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Pig carcass character GFAT1 gene clone and its application

A pig carcass and gene technology, applied in the fields of application, genetic engineering, plant genetic improvement, etc., can solve the problems of limited, limited number of new genes, few genes, etc.

Inactive Publication Date: 2008-02-27
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Although some important progress has been made in the study of candidate genes for carcass traits of pigs, there are still some shortcomings: (1) The number of new genes identified is limited, and few genes can be applied to actual production
(2) The important economic traits of pigs are usually quantitative traits, and the physiological and biochemical processes involved are quite complex. Even for the same quantitative trait, although 1-2 of its controlled genes have been revealed, there are still other new genes with large effects. genes to be discovered
(3) The current research on quantitative trait genes basically selects a single candidate gene for analysis, ignoring the interaction between genes
One of the contents of modern molecular breeding is genome breeding, which is based on the overall or whole genome selection, mating, species conservation and heterosis analysis and utilization based on the organizational structure and functional effects of the genes and genotypes of all traits of the individual. A complete high-density gene map, and a full understanding of the organizational structure of all genes, the mechanism of functional expression regulation, and the association with traits. However, the genes and markers that have been genetically and physically mapped in pigs are still very limited. Work in this area still needs to be further strengthened
(3) The method of finding genes with important physiological functions is not comprehensive enough, the efficiency is not high, and innovation is needed, and it is necessary to further search and excavate new genes related to important economic traits of pigs

Method used

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  • Pig carcass character GFAT1 gene clone and its application
  • Pig carcass character GFAT1 gene clone and its application
  • Pig carcass character GFAT1 gene clone and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] 1. Cloning of GFAT1 gene:

[0048] (1) Primer design:

[0049] Using human GFAT1 gene cDNA (GenBank accession number: NM_002056) as an information probe, using the BLAST tool in NCBI to perform homologous sequence screening in the GenBank pig EST database, and obtain a series of ESTs with a homology of more than 85% (the fragment length is greater than 100bp), query the corresponding sequences with the accession numbers of these ESTs in NCBI with ENTREZ (http: / / www.ncbi.nlm.nih.gov / Web / Search / index.html), and then use the ASSEMBLY program in GeneTool to construct pig EST-contig. Design four pairs of primers GF-A according to EST splicing sequence 1 and GF-B 1 , GF-A 2 and GF-B 2 , GF-A 3 and GF-B 3 , and GF-A 4 and GF-B 4 Amplified to obtain four fragments A 1 B 1 ;A 2 B 2 ;A 3 B 3 and A 4 B 4 (as shown in Table 3). The PCR product was recovered and purified, cloned and sequenced, and the sequence was spliced ​​to obtain the complete coding region sequ...

Embodiment 2

[0098] The distribution situation of embodiment 2 PCR-RFLP-MvaI polymorphism in each pig breed

[0099] Five pig herds were detected by PCR-MvaI-RFLP: Tongcheng purebred (Chinese local pig blood) population, large white purebred (foreign blood), Landrace purebred (foreign pig blood), Changtong (Chinese and foreign hybrid pig blood) And Dachangtong (Chinese-foreign hybrid pig bloodline), the gene frequency of each pig group is shown in Table 6, and the genotypes of GFAT1 gene are distributed in Landrace and Dachangtong pigs, and GG was not detected in Large White and Changtong pigs Homozygous genotype, only AA homozygous genotype in Tongcheng pigs.

[0100] Table 6 GFAT1 gene PCR product genotype frequency and gene frequency

[0101]

Embodiment 3

[0102] Example 3 Application of Association Analysis of Pig Marker Traits

[0103] The association analysis between MvaI-RFLP polymorphism in the 8th intron of pig GFAT1 gene and some production traits was carried out in a Tongcheng pig herd. The analysis results showed that the carcass length and fat thickness at the midpoint of the gluteus medius were significantly different between GA and GG genotypes (P<0.05) (Table 7)

[0104] It can be seen from Table 7 that the G allele is a favorable marker for carcass straight length and fat thickness at the midpoint of the gluteus medius (long carcass, thin rump fat), and GG genotype markers can be used to increase body length and reduce gluteus medius at the same time. The selection mark for the fat thickness at the point.

[0105] Table 7 Association analysis of different GFAT1 gene MvaI-RFLP genotypes and some production traits

[0106] genotype

[0107] Note: The same letter in the same column means no significant dif...

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Abstract

The invention discloses a pig carcass deseription GFAT1 gene clone and polymorphism detection to mark auxiliary breeding in the animal genetic engineering technical domain, which is characterized by the following: the cDNA sequence of the pig carcass deseription GFAT1 gene is displayed as SEQ ID NO: 1 and the DNA sequence is depicted as SEQ ID NO: 2; the G101-A101 base at 101st position in the SEQ ID NO: 2 are mutated, which leads MvaI-RFLP polymorphism. The invention also provides a primer of 8th introne of the cloned GFAT1 gene and augumented GFAT1 to detect the polymorphism, which is a new genetic mark and method of polymorphism of the pig carcass deseription GFAT1 gene.

Description

technical field [0001] The invention belongs to the technical field of animal genetic engineering, and in particular relates to the cloning and polymorphism detection of a pig carcass trait GFAT1 gene of a genetic marker used for pig marker-assisted breeding. Background technique [0002] With the continuous growth of people's demand for animal protein food and the development of commodity economy, carcass quality evaluation has become a common concern of geneticists, breeders, producers, meat dealers and consumers. With the development of domestic and foreign live pig and pork product commodity markets and the improvement of scientific and technological level, the standardization and standardization of carcass quality evaluation have become research hotspots. The currently recognized carcass quality evaluation indicators in the world include: carcass lean meat Ratio (lean percentage in the carcass, CLP), lean content (lean content in the carcass, CLC) or marketable lean cut...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/12C07H21/04C12Q1/68C12N15/65
Inventor 余梅刘科赵书红刘榜樊斌朱猛进李长春白雪杜小勇
Owner HUAZHONG AGRI UNIV
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