Immobilized biological agent and method for degrading bice green contamination of cultivation aquifer

A technology of immobilized microorganisms and preparations, applied in biological water/sewage treatment, immobilized on/in organic carriers, chemical instruments and methods, etc., can solve problems such as predation by aquatic organisms and difficulty in achieving satisfactory repair effects, etc. To achieve the effect of ease of use
CN101134954BInactive Publication Date: 2010-09-22FRESHWATER FISHERIES RES INSITUTE OF JIANGSUPROVINCE
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
FRESHWATER FISHERIES RES INSITUTE OF JIANGSUPROVINCE
Publication Date
2010-09-22
Estimated Expiration
Not applicable · inactive patent

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Abstract

The present invention is one kind of immobilized microbial preparation and the method of degrading malachite green pollutant in aquiculture water therewith. The immobilized microbial preparation is prepared with Pantoea sp. M3 strain as effective component and through the following steps: 1. inoculating M3 seed to enrichment culture medium, culturing at 30-37 deg.c and 150 rpm for 18-30 hr, centrifuging, and washing with phosphate buffer to obtain concentrated bacterial suspension; 2. dissolving sodium alginate in water through heating, cooling to room temperature, mixing with the concentrated bacterial suspension; 3. dropping the mixture into 5 % concentration CaCl2 solution to form calcium alginate pellets and cross-linking to fix at 4-8 deg.c; and 4. washing the calcium alginate pellets with phosphate buffer. The present invention has high malachite green pollutant eliminating rate.
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Description

technical field

[0001] The invention relates to the immobilization technology of Pantoea sp. M3 bacterial strain, and also relates to the microbe restoration technology of malachite green polluted bottom mud. Background technique

[0002] Malachite Green (Malachite Green, MG) is a synthetic triphenylmethane dye, which has been widely used as an insect repellent and fungicide to kill parasites, protozoa and molds in fish eggs outside of aquatic animals. Wait. However, malachite green and its metabolite cryptomalachite green are easy to accumulate in organism tissues, are not easy to metabolize, and have high carcinogenic, teratogenic and mutagenic effects. Therefore, many countries such as the United States, Canada, Japan, and the European Union have listed it as a banned drug for aquaculture. my country also included malachite green in the "List of Veterinary Drugs and Their Compounds Prohibited for Food Animals" in May 2002. However, since malachite green is quickly abso...

Claims

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