Light-induction dielectrophoresis auxiliary unicellular dielectric spectrum automatic test equipment and testing method

A technology of light-induced dielectrophoresis and automatic testing device, which is applied in biological testing, biochemical equipment and methods, diagnostic recording/measurement, etc. Active single cell large-scale parallel control and other problems, to achieve the effect of large-scale parallel control and broad application prospects

Inactive Publication Date: 2008-03-05
SOUTHEAST UNIV
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Problems solved by technology

[0008] Technical problem: The present invention provides a light-induced dielectrophoresis-assisted single-cell dielectric spectrum automatic test device and test method to solve the problem that in the prior art, the large-scale parallel control of active single cells cannot be realized; the high-throughput, Defects of fully automatic sampling, testing and separation

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  • Light-induction dielectrophoresis auxiliary unicellular dielectric spectrum automatic test equipment and testing method
  • Light-induction dielectrophoresis auxiliary unicellular dielectric spectrum automatic test equipment and testing method
  • Light-induction dielectrophoresis auxiliary unicellular dielectric spectrum automatic test equipment and testing method

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Embodiment Construction

[0030] The present invention provides a complete set of devices and methods suitable for single-cell dielectric spectroscopy testing. The light-induced dielectrophoresis-assisted automatic single-cell dielectric spectroscopy test method is based on the measurement of cell movement speed and rotation speed by machine vision technology. It is obtained by collecting and digitally processing images at every moment in the process of cell movement. This light-induced dielectrophoresis-assisted single-cell dielectric spectroscopy automatic test method is mainly based on the light-induced dielectrophoresis force to achieve parallel capture, sample injection, positioning, axis rotation and final separation of single cells. Applying alternating voltage signals of equal intensity and 90 degrees of adjacent phase difference to the microelectrodes forms a rotating electric field to make the cells rotate under the action of the rotating electric field. Based on machine vision, pattern recogniti...

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Abstract

The invention comprises: a photoinduction dielectrophoresis chip, machine vision unit, a vision servo unit and a virtual electrode writing unit. Said photoinduction dielectrophoresis chip is located on a supporting unit and is used for placing the simple cell suspension; said photoinduction dielectrophoresis chip is a three layer hamburger structure; said three layer structure comprises a conducting glass layer, a micro channel layer and a photoinduction chip; on the conducting glass, respectively setting an inlet and an outlet.

Description

Technical field [0001] The invention relates to a single-cell dielectric spectrum test device and a test method, in particular to an electrorotating single-cell dielectric spectrum test device and a test method. Background technique [0002] Cells are the basic unit of the morphological structure and life activities of organisms. To understand the laws of life activities, people must base on the study of cells, investigate the structure and function of cells, and explore the laws of life activities. [0003] Malignant tumors, also called cancers, are the most serious diseases that endanger human health. In the United States, the mortality rate of malignant tumors is second only to cardiovascular disease. According to the 2006 Statistical Bulletin on the Development of Health Services in my country, malignant tumors are the first cause of death for residents in urban areas, followed by cerebrovascular disease and heart disease. [0004] If cancer cells can be found at the single-c...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/48C12Q1/02A61B5/00A61B10/00
Inventor 倪中华朱树存易红宋春峰
Owner SOUTHEAST UNIV
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