Tissue engineering ligament recovering material and its preparation method

A repair material and tissue engineering technology, applied in the field of medicine and biomedical engineering, can solve the problems of high price, complicated surgical operations, and the necessity of correction, and achieve the effect of good biomechanical performance and good biocompatibility

Inactive Publication Date: 2008-03-26
CHONGQING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In addition, the need for correction of ACL reconstruction increases with primary A

Method used

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  • Tissue engineering ligament recovering material and its preparation method
  • Tissue engineering ligament recovering material and its preparation method

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preparation example Construction

[0048] The preparation method of the tissue engineered ligament repair material of the present invention is simple, a certain amount of ligament cells are mixed with a pharmaceutically acceptable biodegradable material, and then the surface of the guide body is combined with ligament healing promoting factors with optimized controlled release kinetics or / and ligament fibroblasts or stem cells.

[0049] The shape of the tissue-engineered ligament graft of the present invention is not particularly limited, and can be shaped arbitrarily according to the shape of tissue defect.

[0050] The diameter of the nano minimally invasive guide in the present invention is preferably less than 500 nm.

[0051] The concentration of ligament cells or bone marrow stromal stem cells in the tissue engineered ligament graft of the present invention is usually 0.5×10 6 / ml to 5×10 8 / ml or higher, preferably 5×10 6 / ml to 5×10 8 / ml.

[0052] In addition, in the tissue engineered tendon graft...

Embodiment 1

[0056] Example 1 The acquisition of bone marrow stromal stem cells

[0057] Seeding cells is an essential element in tissue engineering research. The application of tissue engineering technology to construct ligament tissue requires the expansion and culture of as few cells as possible in vitro to reach the number of cells required for the construction of tendon tissue.

[0058] Bone marrow stromal stem cells were obtained from Cambrex (Walkersville MD), and the markers on the cell surface were positive for CD44 and negative for CD34 by flow cytometry (see Figure 2), so the purified bone marrow stromal stem cells were isolated. They were cultured in MSCGM medium (Cambrex), saturated humidity, 5% CO2, 37°C in a constant temperature shaker.

[0059] The subculture steps of bone marrow stromal stem cells include:

[0060] Aspirate all the culture medium in the culture dish, wash it with PBS; add 3ml of 0.25% trypsin, put it in a 37°C incubator for digestion for 2 minutes; obser...

Embodiment 2

[0062] Preparation of embodiment 2 import body

[0063] After the copolymer of PLA and PGA is sprayed by an electroplating spinning forming apparatus, and then subjected to a uniaxial stretching apparatus, the nanofiber filaments are arranged in an orderly manner to form an aggregate of materials for the import body.

[0064] Fig. 3 is a scanning electron microscope image of the fabricated nano-stent, in which the left image shows that the nano-fiber filaments are disorderly arranged to form a nano-introducer. The picture on the right shows that after uniaxial stretching, the nanofiber filaments are arranged in an orderly manner to form a nanointroducer. It is best to use nanofiber aggregates arranged orderly after uniaxial stretching for the introduction body.

[0065] The preparation of the above-mentioned import body is an important aspect of tissue engineering technology. In the past, carbon fibers and braids were used as scaffolds in tendon tissue engineering. However,...

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Abstract

The present invention provides a kind of tissue-engineered ligament repairing material. Said ligament repairing material includes (a), nano-grade microentrainment body; (b), pharmaceutically-acceptable biological degradable material; and (c), ligament reunion-promoting factor or/and ligament fibroblast or stem cell which are combined on the surface of said microentrainment body. Said invention can be used for effectively curing ligament injury. Besides, said invention also provides its concrete preparation method and steps.

Description

technical field [0001] The invention relates to the fields of medicine and biomedical engineering, and more specifically relates to a tissue engineering material, a preparation method thereof and an application as a ligament repair material. Background technique [0002] Ligaments are dense connective tissues that connect bones and are widely distributed in the body, especially near joints. Its function is to stabilize the joints and prevent excessive flexion and extension of the joints. Its composition is mainly solid and thick collagen fibers and a small amount of aminoglycans. [0003] From a macroscopic point of view, ligaments are synthetic materials that secrete collagen and elastin from fibroblasts and organize them into fibers, and consist of fibroblasts. Fibroblasts are scattered on collagen fiber bundles in the matrix. Containing water, it can carry out the transportation of nutrients, and lubricate or isolate collagen fibers by interacting with macromolecules in...

Claims

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Application Information

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IPC IPC(8): A61L27/54A61L27/14
Inventor 杨力李松蒋稼欢宋国立李苑
Owner CHONGQING UNIV
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