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A breeding method of root cancer resist cherry rootstock sprout as well as group culturation rapid propagating technology

A technology for root cancer and rootstock seedlings, applied in the direction of recombinant DNA technology, other methods of inserting foreign genetic materials, gardening methods, etc., can solve the problems of fruit farmers' economic losses, poor fruit quality, and easy death of large trees, and achieve good growth , to meet the effect of mass production

Inactive Publication Date: 2008-04-16
北京市海淀区植物组织培养技术实验室
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The incidence of root cancer of cherry rootstocks commonly used in production is high, resulting in weak tree vigor, poor quality of results, and easy death of large trees, causing great economic losses to fruit farmers
To a certain extent, it also limits the development speed of the cherry industry.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0088] 1, explant is the selection of anti-root cancer cherry rootstock test-tube seedling:

[0089] Take early spring hydroponic new shoots or new shoots of greenhouse seedlings. When the new shoots grow to about 10 cm, cut the new shoots for explant materials for tissue culture and rapid propagation;

[0090] The obtained aseptic test-tube plantlets were screened for root cancer resistance, and the root-cancer-resistant cherry rootstock test-tube plantlets were selected. The steps were as follows: After inoculating the middle and lower part of the stems of the sterile cherry rootstock test-tube plantlets with Agrobacterium, place them in a hormone-free MS culture. Carry out cultivation in the base, choose the anti-root cancer cherry rootstock test-tube plantlet of 0 grade according to the weight classification of gall tumor after 40 days as the material of follow-up operation;

[0091] 2. Propagation and cultivation:

[0092] After the selection of explants in step 1, after...

Embodiment 2

[0100] Using the same steps as in Example 1, in step 2, the conditions for the propagation of test-tube plantlets were changed to medium MS+6BA 1.0mg / L, the temperature was controlled at about 22°C, the light intensity was 1800Lx, and the light was 12 hours. In 3, change the strong seedling culture condition into strong seedling medium MS+GA 7mg / L, strong seedling 30 days, the rooting culture condition in the step 4 becomes in the rooting medium of MS+NAA 0.5mg / L.

Embodiment 3

[0102] Using the same steps as in Example 1, in step 2, the conditions for the propagation of test-tube plantlets were changed to medium MS+6BA 1.1mg / L, the temperature was controlled at about 24°C, the light intensity was 2000Lx, and the light was illuminated for 12 hours. In 3, change the strong seedling culture condition into strong seedling medium MS+GA 8mg / L, strong seedling 31 days, the rooting culture condition in the step 4 becomes in the rooting medium of MS+NAA 0.5mg / L.

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PUM

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Abstract

A breeding method of cherry rootstock seedling with crow gall resistance concretely relates to a method which used tissure culture and rapid propagation to quickly screen and propagate cherry rootstock seedlings with crow gall resistance, belonging to propagating and screening methods of fruit trees in agriculture. The method includes a plurality of steps of the selection of aseptical cherry rootstock tube seedlings with crow gall resistance, the proliferation, propagation and cultivation of tube seedlings, the cultivation of strong tube seedlings, the cultivation of tube seeding root occurance and the transplantation cultivation of tube seedlings. Because the technical scheme of the invention is applied, the invention has a plurality of beneficial effects: first, character-steady cherry rootstock seedlings with crow gall resistance are obtained quickly and enormously with lower cultivation-cost and incidence of cherry rootstock crow gall; second, the growth potential is stonger than before and the quality is better; third, big trees are not easy to die again.

Description

Technical field: [0001] The invention belongs to a method for fruit tree propagation and selection in agriculture, and in particular relates to a method for rapidly screening and breeding root cancer-resistant cherry rootstock seedlings by adopting tissue culture rapid propagation technology. Background technique: [0002] At present, the distribution of sweet cherries in my country is mainly concentrated on the coast of Bohai Bay, with the most in Yantai City and the suburbs of Dalian City. Shandong Province is the province with the largest planting area and the largest output of sweet cherries in my country. In addition to the districts and counties of Yantai City, it is also distributed in Qingdao, Weihai, Jinan, Rizhao, Zibo, Weifang, Zaozhuang, Tai'an, Linyi and other places. Liaoning Province is concentrated in Jinzhou District and Ganjingzi District of Dalian City. Hebei Province is mainly distributed in Shanhaiguan District, Beidaihe District and Changli County of Q...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01H3/00C12N5/04C12N15/82C12N15/87A01G31/00C12N15/84
Inventor 刘兰英李春玲邵磊张军民
Owner 北京市海淀区植物组织培养技术实验室