A method for rapid propagating China red nouelia

A fast and fast technology of Rhizoma sinensis, which is applied in the field of plant regeneration of tissue culture technology, can solve the problems of slow multiplication speed of tissue culture seedlings, low survival rate of transplanting, difficulty in rooting, etc., and achieves simple and easy cultivation method, good growth effect, light browning effect

Inactive Publication Date: 2008-04-16
北京市海淀区植物组织培养技术实验室
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Problems solved by technology

[0005] The main purpose of the present invention is to aim at the problems such as the easy pollution and browning of the explants after inoculation existing in the above-mentioned red sinensis tissue culture rapid propagation technology, slow propagation speed of tissue culture seedlings, difficulty in rooting, and low survival rate of transplanting. The study of culture medium and culture conditions has realized the low pollution rate, high differentiation rate, high rooting rate and high survival rate of red cotinus sinensis tissue culture and rapid propagation

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  • A method for rapid propagating China red nouelia

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Embodiment 1

[0034] (1) Selection of explants

[0035] Use grafting or cuttings to propagate a small amount of Sinus sinensis seedlings, spray fungicides regularly, and plant Sinus sinensis in the greenhouse in late January or early February of the following year. At the end of February and early March, the new shoots grow to 15-25 cm, cut the new shoots and cut them into 1.5-2.5cm stem segments, rinse them with running water first, shake them with 70% alcohol for 15-50 seconds on an ultra-quiet platform, and then place them in 0.2% mercury chloride and 10% calcium hypochlorite for 3-8 minutes, and finally rinsed with sterile water for 3-5 times, as explants for tissue culture and rapid propagation of Red Sinensis; the experimental results showed that using 0.2% mercuric chloride Used in conjunction with 10% calcium hypochlorite, the disinfection effect is good, the pollution rate is only 24.2%, and the pollution rate is low. The new shoots cultivated in the greenhouse in February and Marc...

Embodiment 2

[0045]In the cultivation process of tissue culture seedlings in this embodiment, the inoculation medium used is 1 / 2MS basic medium with the addition of 0.5mg / L of BA and 0.1mg / L of NAA; the used expansion medium is 1 / 2MS basic The BA of 1.0mg / L and the NAA of 0.1mg / L are added to the culture medium; The rooting medium used is the IBA of 0.5mg / L added to the 1 / 2MS basal medium; In the present embodiment, other cultivation steps are the same as in Example 1 .

Embodiment 3

[0047] In the cultivation process of the tissue culture seedlings in this embodiment, the inoculation medium used is 1 / 2MS basic medium with the addition of 1.5mg / L of BA and 1.0mg / L of NAA; the used expansion medium is 1 / 2MS basic The BA of 2.5mg / L and the NAA of 1.0mg / L are added in the medium; The rooting medium used is the IBA of 2.5mg / L added to the 1 / 2MS basic medium; In the present embodiment, other cultivation steps are identical with embodiment 1 .

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Abstract

The invention encloses a fast-propagation method which uses tissure culture and rapid propagation to obtain chinese red coggygria tissure culture seedings. The chinese red coggygria is a mutant strain detected from continus coggygira seedlings, which has little seed source and is hard to be cultivated by conventional method and cannot meet produce damand. The cultivation method and acquisition period of explant, disinfection method, the screening of differentiation medium, proliferation medium, and rooting medium, the selection of transplantation medium, environment condition in the process of cultivation, and other aspects are controlled in the invention. A complete tissure culture and rapid propagation system of chinese red coggygria is established by using a transitional cultivation method of explant selection, inoculation cultivation, proliferation cultivation, rooting cultivation, ensuring the differentiation rate of chinese red coggygria up to 100%, proliferation multiple per month up to 5.2, rooting rate up to 91.7%, transplanting survival rate up to over 85%. The invention with simple and convenient method has a simply operation procedure, high efficiency, which effectively inhibits the polution and browning of chinese red coggygria explant, and improves the differentiation rate of tissue culture seedling. The industrial production is prone to be achieved with a plurality of characteristics of high proliferation rate, high rooting rate, high survival rate, low cost.

Description

technical field [0001] The invention belongs to the technical field of plant regeneration of tissue culture technology, and in particular relates to a method for rapid propagation of Rhizoma sinensis. Background technique [0002] Cotinus chinensis is a mutated single plant found in cotinus cotinus seedlings. It is red all year round and has a good ornamental effect; it is resistant to drought and low temperature. It is in the natural conditions of 40-42 ℃ in summer and -22.8 ℃ in winter in Xishan area of ​​Beijing It can grow normally without pulling strips; the soil requirements are not strict, and it can grow in barren, neutral, weakly acidic, and weakly alkaline soils, so the seedlings are very popular in the market. However, due to the lack of provenance and the slow propagation by conventional methods, it cannot meet the needs of production, so we carried out the research on the rapid propagation technology of Cotinus sinensis tissue culture. [0003] The main technic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00C12N5/04A01G31/00
Inventor 刘兰英李春玲张军民
Owner 北京市海淀区植物组织培养技术实验室
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