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Manganese diagnosis/measuring reagent kit and method for measuring manganese concentration

A method for determining manganese concentration and a technology for manganese diagnosis/determination kits and the field of manganese concentration determination

Inactive Publication Date: 2008-05-07
SUZHOU ANJ BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Neutron excitation analysis (NAA) is the best method, but can only be determined in some well-conditioned laboratories

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] The manganese diagnosis / measurement reagent of this embodiment is a single reagent, including:

[0038] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0039] Stabilizer 50mmol / L

[0040] Reduced coenzyme 0.25mmol / L

[0041] Oxaloacetic acid 25mmol / L

[0042] Ammonia ion 40mmol / L

[0043] Oxaloacetate decarboxylase 12000U / L

[0044] Alanine dehydrogenase 16000U / L

[0045] After the reagents are all dissolved and prepared, they are divided into bottles and freeze-dried to make dry powder reagents; before use, add purified water and reconstitute for use.

[0046] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance 1.8±0.7, test main wavelength 340nm, test subwavelength 405nm, volume ratio of manganese sample to reagent is 1 / 25, The reaction direction is negative reaction (decreasing reaction), the delay time is about 1 minute, and the detection time is about 2 minutes.

[0047] After adding ...

Embodiment 2

[0049] The manganese diagnosis / measurement reagent of the present embodiment is a double reagent, comprising:

[0050] Reagent 1

[0051] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0052] Stabilizer 50mmol / L

[0053] Reduced coenzyme 0.25mmol / L

[0054] Oxaloacetic acid 20mmol / L

[0055] Ammonia ion 30mmol / L

[0056] Reagent 2

[0057] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0058] Stabilizer 50mmol / L

[0059] Oxaloacetate decarboxylase 16000U / L

[0060] Alanine dehydrogenase 24000U / L

[0061] After the reagents are all dissolved and prepared, they are divided into bottles to make liquid double reagents, which can be used directly.

[0062] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance 1.8±0.7, test main wavelength 340nm, test sub-wavelength 405nm, the volume ratio of the tested manganese sample to reagent 1 and reagent 2 is 2 / 20 / 5, the reaction directio...

Embodiment 3

[0065] The manganese diagnosis / measurement reagent of the present embodiment is three reagents, comprising:

[0066] Reagent 1

[0067]Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0068] Stabilizer 50mmol / L

[0069] Reduced coenzyme 0.25mmol / L

[0070] Ammonia ion 20mmol / L

[0071] Reagent 2

[0072] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0073] Oxaloacetic acid 40mmol / L

[0074] Reagent 3

[0075] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0076] Stabilizer 50mmol / L

[0077] Oxaloacetate decarboxylase 24000U / L

[0078] Alanine dehydrogenase 36000U / L

[0079] After all the reagents are dissolved and prepared, they are divided into bottles to make liquid three reagents, which can be used directly.

[0080] When measuring manganese concentration, set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance 1.8±0.7, test main wavelength 340nm, te...

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Abstract

The invention relates to a manganese diagnostic / testing reagent kit using enzymatic colorimetric method and enzymic linkage technology as well as method for testing concentration of manganese as well as composition and content of the reagent, belonging to technical field of medicine / food / environment test. The invention is characterized in that composition in the reagent kit comprises buffer solution, reduction coenzyme, oxaloacetic acid, ammonium, oxaloacetic acid decarboxylase, alanine dehydrogenase and stabilizing agent; the sample is mixed with the reagent according to specified ratio to generate a series enzymatic reactions, then the reactant is arranged under a ultraviolet / visible light analyzer to test the decrease speed of absorbance at wavelength of 340nm, thereby the manganese concentration can be tested. The invention has the advantages that the test results can be easily obtained using ultraviolet / visible light analyzer.

Description

technical field [0001] The invention relates to a manganese diagnosis / measurement kit, and at the same time, the invention also relates to a method for measuring manganese concentration, which belongs to the technical field of medicine / food / environmental inspection and determination. Background technique [0002] As a trace element, manganese is an indispensable and necessary component in the human body, and it is also a substance that is toxic to the human body. In the natural state, it exists in divalent, trivalent and tetravalent forms, and the lower the degree of oxidation, the higher the toxicity. There are generally eight different oxidation states of manganese. The biological significance of manganese is its diverse functions as a cofactor in the human body. However, in the activation of many enzyme reactions, Mn 2+ and Mg 2+ Can replace each other. [0003] Commonly used graphite furnace atomic absorption spectrophotometry, atomic emission ICP-OES. Neutron exci...

Claims

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Application Information

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IPC IPC(8): G01N33/84G01N21/78G01N21/25C12Q1/32C12Q1/00
Inventor 王尔中
Owner SUZHOU ANJ BIOTECHNOLOGY CO LTD
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