Method for rapid purification of codonopsis pilosula lobetyolin monomer from codonopsis pilosula medicinal materials

A technology for the separation and purification of tangshenoside, applied in chemical instruments and methods, organic chemistry, preparation of sugar derivatives, etc., can solve the problems of large solvent consumption, long time consumption, cumbersome solvent recovery, etc., and achieve low solvent consumption and short process cycle Short, solvent-intensive and labor-intensive results

Inactive Publication Date: 2008-05-14
CHENGDU PUSH BIOLOGICAL TECH
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  • Abstract
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  • Claims
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AI Technical Summary

Problems solved by technology

The current bibliographical report (He Qing etc. people) the method of the tangshenyloside is: repeated column chromatography after sub-section extraction, comprise

Method used

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  • Method for rapid purification of codonopsis pilosula lobetyolin monomer from codonopsis pilosula medicinal materials
  • Method for rapid purification of codonopsis pilosula lobetyolin monomer from codonopsis pilosula medicinal materials

Examples

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example 1

[0024] A method for rapidly separating and purifying tangshenoside monomers from Codonopsis pilosula medicinal materials produced in Shanxi, the following steps are carried out at 55°C:

[0025] A. Cold extraction: crush the dried roots of Codonopsis pilosula through a 60-mesh sieve, mix and soak them with ethanol for 24 hours, cold soak and filter to remove slag, and the obtained liquid is recovered under reduced pressure to remove ethanol to obtain a concentrated extract; the mixing ratio is the volume of Codonopsis pilosula powder and The volume ratio of ethanol or methanol is 1:3. The ethanol concentration was 95%.

[0026] B. Coarse separation of resin: After diluting the concentrated extract in the previous step with water, filter it with a fine gauze cloth of about 200 meshes to remove solid impurities such as sand and dust before applying the macroporous resin, and then pass it into the activated macroporous adsorption resin column, elute the macroporous adsorption re...

example 2

[0033] A method for rapidly separating and purifying tangshenoside monomers from Codonopsis pilosula medicinal materials produced in Shanxi, the following steps are carried out at 25°C:

[0034] A. Cold soaking extraction: mix and soak the dried tuber root powder of Codonopsis pilosula and ethanol for 36 hours, remove the slag by cold soaking and filtration, and recover the obtained liquid under reduced pressure to remove ethanol to obtain a concentrated extract; the mixing ratio is that the volume ratio of Codonopsis pilosula powder and ethanol is 1: 3; The concentration of ethanol is 100%, and the particle size of the dried tuber root powder of Codonopsis pilosula is 40 mesh.

[0035] B. Coarse separation of resin: After diluting the concentrated extract in the previous step with water, filter it with a fine gauze cloth of about 200 meshes to remove solid impurities such as sand and dust before applying the macroporous resin, and then pass it into the activated macroporous ad...

example 3

[0040] A method for rapidly separating and purifying tangshenoside monomers from Codonopsis pilosula medicinal materials produced in Shanxi, the following steps are carried out at 58°C:

[0041] A. Cold soaking extraction: Mix and soak the dried root tuber powder of Codonopsis pilosula and ethanol for 30 hours, then filter and remove the slag by cold soaking, and recover the obtained liquid under reduced pressure to remove ethanol to obtain a concentrated extract; the mixing ratio is that the volume ratio of Codonopsis pilosula powder and ethanol is 1: 3; The concentration of ethanol is 100%, and the particle size of the dried tuber root powder of Codonopsis pilosula is 100 mesh.

[0042] B. Coarse separation of resin: After diluting the concentrated extract in the previous step with water, filter it with a fine gauze cloth of about 200 meshes to remove solid impurities such as sand and dust before applying the macroporous resin, and then pass it into the activated macroporous ...

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Abstract

The invention relates to a method for rapidly separating and purifying tangshenoside monomers from Codonopsis pilosula medicinal materials. It includes four steps, 1. Cold soaking extraction: cold soaking with ethanol or methanol; 2. Coarse resin separation: load the sample into the activated macroporous adsorption resin column, divide it into three parts with water, 30% ethanol, and 50%-70% ethanol. 3. Chromatographic separation: After diluting the sample with acetonitrile-water mobile phase, inject the sample, and collect the effluent in sections; 4. Decolorization and purification: load the sample on the polyamide resin In the process, the water eluate is concentrated, and 98.5% high-purity tangshenoside product is recovered. The method is simple and fast, consumes less solvent, is economical and environmentally friendly, and has a high yield, is suitable for industrial production, and has high economic value and academic value.

Description

technical field [0001] The invention relates to a process for rapidly separating and purifying tangshenoside monomers from medicinal material Codonopsis pilosula in an industrialized manner. Background technique [0002] Codonopsis pilosula (Franch.) Nannf., Codonopsis pilosula Nannf.var.modesta (Nannf.) L.T.Shen or Codonopsis pilosula (Franch.) Nannf., Codonopsis pilosula Nannf.var. There are a variety of products due to different places of origin and types, such as Ludangshen, Taiwan Codonopsis, Dongdangshen and Wendangshen, etc., all have the effects of nourishing the middle and nourishing Qi, strengthening the spleen and lungs, etc. They all contain the active ingredient tangsheyne, The 2005 edition of the Pharmacopoeia has changed Codonopsis polysaccharide as the characteristic component to Codonopsis glycoside as the characteristic component of TLC identification. In order to better control the internal quality of Codonopsis pilosula, the demand for Codonopsis glucosid...

Claims

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Application Information

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IPC IPC(8): C07H15/203C07H1/08
Inventor 杨兵张黎董维珍刘丁
Owner CHENGDU PUSH BIOLOGICAL TECH
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