Method for preparing phytase taking glycerol as carbon source

Abstract

A method for preparing phytase using glycerol as a carbon source, adopting the fermentation production process of phytase-producing genetically engineered bacteria using Pichia pastoris as a carrier, characterized in that glycerol is used as a carbon source, and an appropriate amount of bacterial strains for production is used , cultured in a shake flask in a seed medium, and then through the first-level seed culture, second-level seed culture, and fermentation culture processes, the only carbon source in the growth stage of the bacteria before the seed culture and fermentation enzyme production is glycerol; during fermentation, After the glycerol in the basic fermentation medium is completely consumed, start to feed 50% glycerol solution into the medium. After the glycerol is exhausted, start to feed methanol to induce phytase production. Methanol also acts as a carbon source in the production stage. The positive effect is: Glycerin as a carbon source increases the final phytase production by 20-30%, increases the enzyme activity by 20-30%, the color of the fermentation liquid is light and does not require decolorization treatment, and reduces production costs. This method reduces costs compared with traditional processes About 1/3.

Description

technical field [0001] The invention relates to a fermentation technology of microbial products and a method for preparing phytase using glycerol as a carbon source. Background technique [0002] The traditional method of producing phytase is to use glucose as carbon source. Glucose, as a carbon source, produces a caramelization reaction during the sterilization process, making the fermentation medium solution brown or soy sauce-colored. Its disadvantages are: first, the caramelization reaction produces harmful substances, which is not conducive to the growth of yeast; second, the post-processing of the dark fermented liquid requires decolorization, which increases the production process and costs. Contents of the invention [0003] The purpose of the present invention is to provide a method for preparing phytase using glycerol as a carbon source, which can significantly increase the output of phytase by 20%-30%, without decolorization, and reduces production costs. [0...

AI Technical Summary

Problems solved by technology

Its disadvantages are: first, the caramelization reaction produces harmful substances, which is not conducive to the growth of yeast; second, the post-processing of the dark fermented liquid requires decolorization, which increases the production process and cost

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