Boar semen freezing preservation tube and method thereof

Abstract

The present invention relates to a porcine semen freezing and storing tube and a porcine semen freezing and storing method thereof. The porcine semen freezing and storing tube comprises a storing tube and is characterized in that the storing tube is a double-layer tube; the two vessel openings of the double-layer tube are opposite. The key points of the porcine semen freezing and storing method are that the frozen and diluted porcine semen after the pretreatment and the cooling treatment is cased into the annular vessel of the porcine semen freezing and storing tube of the present invention; then the porcine semen freezing and storing tube is inserted at liquid nitrogen to be suffocated for a plurality of minutes and directly put into the liquid nitrogen for long time storage, which can increase the contacting area of the semen and the liquid nitrogen during the process of freezing, accelerate semen freezing velocity, reduce the damage to the semen caused by freezing and improve the live-rate of frozen semen. The present invention also has the advantages of low cost, convenient operation, high efficiency, ensuring fecundation rate, etc.

Description

technical field [0001] The invention relates to a pig semen cryopreservation tube and a pig semen cryopreservation method. Background technique [0002] In my country, pigs are the most important livestock, and pork production accounts for about 70% of the total meat in the country. With the improvement of people's living standards, domestic and foreign markets have gradually transformed from quantity to quality (such as meat quality, etc.) to the requirements of commercial pigs. The direction of pig breeding has also changed from mainly focusing on the improvement of growth rate and lean meat percentage to improving the quality of pork and improving the flavor of meat. The traditional breeding method has a long generation interval and cannot meet the market demand. However, the pig frozen semen technology can make full use of excellent breeding stock, shorten the generation interval of fine breed selection, and quickly establish a new hybrid of breeding pigs and commercial...

AI Technical Summary

Problems solved by technology

However, pig frozen semen has been difficult to be widely used in production. There are two reasons. One is that the ratio of cholesterol to phospholipid in the plasma membrane of pig sperm cells is significantly lower than that of herbivores, and the ion permeability of the sperm membrane is enhanced during the freezing process. The loss of lipids leads to low survival rate of frozen semen and low conception rate

The second is that the amount of semen in one mating of pigs is large (about 50 ml), and traditional frozen semen formulations such as ampoules (about 1-2 ml), granules (about 0.5 ml), and thin tubes (about 0.25 ml) are used. A considerable amount (about 10-20 tubes) of frozen semen needs to be thawed, which is not only cumbersome to operate, but also greatly affects the fertilization effect

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