Microwave extraction method for lotus seed protein
An extraction method, lotus seed technology, applied in peptide preparation methods, chemical instruments and methods, organic chemistry, etc., can solve the problems of lye consumption, complicated post-processing, environmental pollution, etc., to improve the extraction rate, shorten the extraction time, The effect of reducing energy consumption
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Embodiment 1
[0014] Salt extraction, 60 mesh lotus seed powder and 0.1mol L -1 The salt solution was uniformly mixed at 1:6, placed in a microwave reactor, microwave emission power 400W, microwave frequency 915MHz, stirring rate 700rpm, repeated salt extraction 3 times, each 25min, centrifugal (2000rpm) 15min; combined supernatant, Concentrate, pass CO 2 In the concentrated solution, acid precipitation pressure 2atm, CO 2 The volume ratio of the feed amount to the concentrated solution is 0.2:1, and the acid precipitation is allowed to stand for 1h. Filter to obtain the filtrate, centrifuge (2000rpm) the filtrate for 15 minutes to obtain the precipitate, combine the filtrate and the precipitate to obtain the lotus seed protein; add water to adjust the lotus seed protein to a 28% feed liquid, spray dry the feed liquid, and the flow rate of the feed liquid is 120kg h -1 , feed liquid temperature 70 ℃, spray inlet temperature 100 ℃, spray outlet temperature 60 ℃, high-purity lotus seed prot...
Embodiment 2
[0016] For salt extraction, 80 mesh lotus seed powder and 0.3mol L -1 The salt solution was uniformly mixed at 1:9, placed in a microwave reactor, microwave emission power 500W, microwave frequency 2450MHz, stirring rate 500rpm, repeated salt extraction 2 times, each 20min, centrifuged (3000rpm) 10min; combined supernatant, Concentrate, pass CO 2 In the concentrated solution, the acid precipitation pressure is 3 atm, CO 2 The volume ratio of the feed amount to the concentrated solution is 0.15:1, and the acid precipitation is allowed to stand for 1.5 hours. Filter to obtain the filtrate, centrifuge (3000rpm) the filtrate for 10min to obtain a precipitate, combine the filtrate and the precipitate to obtain the lotus seed protein; add water to adjust the lotus seed protein to a 38% feed liquid, spray dry the feed liquid, and the flow rate of the feed liquid is 135kg h -1 , feed liquid temperature 80 ℃, spray inlet temperature 120 ℃, spray outlet temperature 70 ℃, get high-puri...
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