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Clematis chinensis medicinal materials effective ingredient content determination method

A technology of active ingredients and determination methods, which is applied in the field of determination of the content of active ingredients in Clematis clematis, can solve the problems that there is no accurate method for determining the content of oleanolic acid and hedera saponin, etc., and achieve the effect of simple operation

Inactive Publication Date: 2008-07-09
INST OF SCI & TECH GUANGDONG UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The content of oleanolic acid and hedera saponin in Clematis clematis is an important parameter to determine the quality standard of Clematis clematis, but there is no method to accurately determine oleanolic acid and hedera saponin in clematis. Determination method of content

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] (1) Preparation of reference substance solution:

[0018] Accurately weigh an appropriate amount of oleanolic acid reference substance and hedera saponin reference substance, add methanol to make a mixed solution with oleanolic acid and hedera saponin concentrations of 1ml / mg, which is the reference substance solution;

[0019] (2) Preparation of the test solution:

[0020] Take 2g of Clematis medicinal material powder, accurately weighed, add 50ml of methanol, ultrasonically treat for 30 minutes, filter, wash the residue with an appropriate amount of methanol, combine the filtrate and washing liquid, recover the solvent until dry, add 10ml of water to dissolve the residue, and wash with saturated normal Shake and extract with butanol 3 times, 20ml each time, combine the extracts, evaporate to dryness, add 20ml methanol and 2ml hydrochloric acid to the residue, heat and hydrolyze for 4 hours, add 10ml water to the hydrolyzate, shake and extract twice with chloroform, 20...

Embodiment 2

[0028] The difference from Example 1 is: step (2) preparation of the test solution: get 2 g of Clematis medicinal material powder, accurately weighed, add 20 ml of methanol, sonicate for 20 minutes, filter, and the residue is washed in an appropriate amount with methanol, combined Filtrate and lotion, recover solvent until dry, add 10ml of water to dissolve the residue, shake and extract twice with saturated n-butanol, 10ml each time, combine the extracts, evaporate to dryness, add 10ml of methanol and 1ml of hydrochloric acid to the residue, heat and hydrolyze for 4 hours , add 10ml of water to the hydrolyzate, shake and extract 3 times with chloroform, 10ml each time, combine the extracts, recover the solvent to dryness, add methanol to dissolve the residue and transfer it to a 10ml measuring bottle, add methanol to the mark, shake well, filter After that, get the continued filtrate to obtain the test solution;

[0029] (3) Accurately draw reference substance solution 5 μ l,...

Embodiment 3

[0036] The difference from Example 1 is: step (2) preparation of the test solution: get 2 g of Clematis clematis powder, accurately weighed, add 100 ml of methanol, sonicate for 25 minutes, filter, the residue is washed in an appropriate amount with methanol, and combined Filtrate and lotion, recover solvent until dry, add 10ml of water to dissolve the residue, shake and extract with saturated n-butanol 4 times, 30ml each time, combine extracts, evaporate to dryness, add 30ml of methanol and 3ml of hydrochloric acid to the residue, heat and hydrolyze for 4 hours , add 10ml of water to the hydrolyzate, shake and extract 4 times with chloroform, 30ml each time, combine the extracts, recover the solvent to dryness, add methanol to dissolve the residue and transfer it to a 10ml measuring bottle, add methanol to the mark, shake well, filter After that, get the continued filtrate to obtain the test solution;

[0037] (3) Accurately draw reference substance solution 5 μ l, 10 μ l, ne...

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Abstract

The invention discloses a method for determining contents of effective components in Clematis chinensis Osbeck, which comprises the following steps of (1) preparing a reference solution; (2) preparing a test sample solution; and (3) precisely extracting the reference solution and the test sample solution respectively, injecting into a liquid chromatograph, and determining by an evaporative light-scattering detector to obtain the contents of effective components including oleanolic acid and hederagenin, wherein the chromatographic filler is selected from octadecylsilane chemically bonded silica, the mobile phase is selected from methanol-water-acetic acid-triethylamine with a volumetric ratio of (80-95):(5-20):(0.01-0.1):(0.01-0.1), and the theoretical plate number, calculated by oleanolic acid, is not less than 2,000. The method has simple operation and can accurately determine the contents of oleanolic acid and hederagenin in Clematis chinensis Osbeck.

Description

technical field [0001] The invention relates to a method for determining the content of effective components in Clematis clematis medicinal materials, in particular to a method for determining the contents of oleanolic acid and hedera saponin in Clematis clematis medicinal materials. Background technique [0002] Clematis is the dry root and rhizome of Clematis chinensis Osbeck, Clematis chinensis Osbeck, Clematis exapetala Pall. or Clematis manshurica Rupr. in the family Ranunculaceae. Spicy and salty in taste, warm in nature. It has the functions of expelling wind and dampness, dredging collaterals and relieving pain, and is used for rheumatic arthralgia, numbness of limbs, spasm of tendons, difficulty in flexion and extension, and bone choking in the throat. [0003] The chemical composition of Clematis clematis is relatively complex. Modern phytochemical and pharmacological studies have shown that oleanolic acid and hedera saponin are the active ingredients of various ...

Claims

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Application Information

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IPC IPC(8): G01N30/02
Inventor 李卫民
Owner INST OF SCI & TECH GUANGDONG UNIV OF CHINESE MEDICINE
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