Method and device for nervus damnification and regeneration renovation by using femtosecond laser

A nerve injury, femtosecond laser technology, applied in the field of cell biology

Inactive Publication Date: 2008-07-16
JIANGSU UNIV
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Problems solved by technology

However, the combination of femtosecond light knife and optical tweezers has not been reported on the nano-damage and regenerative repair of nerve cell axons

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  • Method and device for nervus damnification and regeneration renovation by using femtosecond laser

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Embodiment Construction

[0013] In conjunction with the schematic diagram of femtosecond laser optical tweezers and surgical nerve cell damage and regeneration system device illustrated in Fig. 1, the implementation of the specific device and nerve damage and regeneration method of the present invention is described as follows:

[0014] The device for realizing ultrafast photonic nerve injury and regeneration is mainly composed of a laser generating system, an external optical path system, a high-precision imaging system, and a software control system in sequence. The laser generating system includes a mode-locking knob 1, a femtosecond laser 2, a digital delay / pulse signal generator 7, and a regenerative amplifier 8. The external optical path system includes a movable convex lens 3, a fixed convex lens 4, a rotatable total reflection mirror 5, an attenuation mirror 6, an attenuation mirror 9, a half mirror 10, a shutter 11, a beam expander 12, a dichroic mirror 13 and Microscope objective lens array ...

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Abstract

The invention adopts the femtosecond laser two-photon induced photo knife effect and the high photon density induced optical tweezers gradient force function for carrying out a micro-nano manipulation, which is applicable to the study of the injury, regeneration and repair of various biological cells. The invention consists of a laser generation system, an external optical path system, a high precision imaging system and a software control system. The laser generation system includes two parallel branches, one branch consists of a lock mould knob and a femtosecond laser sequentially, and the other branch consists of a digital delay/pulse signal generator and a regeneration amplifier sequentially. The external optical path system consists of a dual-branch optical path, a semi-transparent semi-reflection mirror, an optical gate and a beam expander sequentially. The dual-branch optical path includes two parts: one part consists of a movable convex lens, a fixed convex lens, a rotatable total reflection mirror and an attenuation mirror sequentially, and the other part is an attenuation mirror. The device can realize the whole process of nano-injury and regeneration study of the nerve cells and can adopt the femtosecond optical tweezers to realize the choice of the super-sophisticated target position during an axon regeneration process.

Description

technical field [0001] The present invention is a cell biology technique, specifically a method and device for nerve damage and regeneration repair using femtosecond laser. The role of micro-nano manipulation is suitable for the research of damage and regeneration of various biological cells. Background technique [0002] With the rapid development of cell biology and molecular biology, the focus of research on nerve cell injury and regeneration has shifted to exploring the local or overall biological factors that regulate nerve regeneration, such as the survival, growth, differentiation, nerve regeneration, and synapse of neurons. Synapse formation, synaptic plasticity and neurodegenerative diseases have become one of the hotspots in the field of neuroscience. [0003] So far, the regeneration of the vertebrate central nervous system after injury is still a major unsolved problem in the field of neuroscience. More and more studies have shown that it is not the inherent ch...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/00C12M1/36
Inventor 周明杨海峰蔡兰
Owner JIANGSU UNIV
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