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Method for extracting four-taxone compounds with high-efficiency abduction

A high-efficiency paclitaxel technology, applied in organic chemistry, fermentation, etc., can solve the problems of weak environmental adaptability, no value of fermentation production, extraction of paclitaxel, etc., and achieve large-scale industrial production. The method is simple and easy to improve Yield effect

Inactive Publication Date: 2008-07-16
NORTHEAST FORESTRY UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the complexity of the synthetic route, there is no commercial application value at present.
Although some paclitaxel-producing fungi have been found, the ability to synthesize paclitaxel is extremely low, and there is no value for fermentation production. The strain selection and improvement work needs to be further carried out
[0005] At present, the number of artificially planted yews is gradually increasing, which provides a new source of paclitaxel, but the growth of yew is slow and the environmental adaptability is weak, so it cannot rely on the bark of artificially planted yew to extract the required paclitaxel. Therefore, It should use its renewable parts - branches and leaves to fully develop and utilize its fresh branches and leaves

Method used

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  • Method for extracting four-taxone compounds with high-efficiency abduction
  • Method for extracting four-taxone compounds with high-efficiency abduction
  • Method for extracting four-taxone compounds with high-efficiency abduction

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Control group: Weigh 20g of fresh twigs and leaves of Taxus chinensis, add 100mL of water to homogenate, filter directly without any treatment, add 100mL of 80% ethanol to the filter residue, extract 3 times under ultrasonic conditions, each time for 30min, combine three times to clear the night, reduce pressure Concentrate until no obvious liquid flows out. The concentrated solution was sequentially extracted three times with n-hexane and chloroform respectively (the volume ratio of the extractant to the concentrated solution was 1:2). The chloroform phase was concentrated and evaporated to dryness. The resulting solid was dissolved in 1 mL of absolute ethanol and added to 20 mL of water. After standing for a while, it was filtered to obtain a white precipitate, which was weighed after drying: 0.026 g. The contents of paclitaxel and cephalomannine are 3.03% and 5.25% respectively. The precipitated aqueous phase was extracted 3 times with chloroform (the volume ratio o...

Embodiment 2

[0026] Control group: Weigh 20g of fresh twigs and leaves of Taxus chinensis, add 100mL of water to homogenate, filter directly without any treatment, add 100mL of 80% ethanol to the filter residue, extract 3 times under ultrasonic conditions, each time for 30min, combine three times to clear the night, reduce pressure Concentrate until no obvious liquid flows out. The concentrated solution was sequentially extracted three times with n-hexane and chloroform respectively (the volume ratio of the extractant to the concentrated solution was 1:2). The chloroform phase was concentrated and evaporated to dryness. The resulting solid was dissolved in 1 mL of absolute ethanol and added to 20 mL of water. After standing for a while, it was filtered to obtain a white precipitate, which was weighed after drying: 0.053 g. The contents of paclitaxel and cephalomanine were 3.38% and 0.72%, respectively. The precipitated aqueous phase was extracted 3 times with chloroform (the volume ratio ...

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Abstract

The invention relates to a method for utilizing enzyme, ultraviolet radiation and oxygen as inductors for the high efficient production of 10-deacetylbaccatin III, baccatin III, cephalomannine and paclitaxel in taxus chinensis branches and leaves by the method of fresh grinding homogenization. The invention takes the fresh branches and leaves of taxus chinensis as raw materials for fresh grinding homogenization, then the enzyme is added, the oxygen is introduced, and a solid is treated with 80 percent ethanol ultrasonic extraction under ultraviolet irradiation. The contents of the 10-deacetylbaccatin III, the baccatin III, the cephalomannine and the paclitaxel in the branches and leaves of the taxus chinensis can be averagely improved by more than 17 percent, 23 percent, 74 percent and 23 percent respectively by the biological induction method. The raw materials used by the invention are the renewable fresh branches and leaves of the taxus chinensis, which can not only ensure no damage to ecological resources, but also can extract the effective components of taxus chinensis and the biological semisynthetic precursor substances with high efficiency. Furthermore, the method has simple and easy operation, which is applicable to industrial application and has great significance for industrial production.

Description

technical field [0001] The invention relates to a method for efficiently inducing and increasing the content of paclitaxel and its semi-synthetic precursors in the fresh branches and leaves of Taxus chinensis. Background technique [0002] Paclitaxel (paclitaxel, figure 1 ) is a diterpenoid compound with a pentamethylpentadecene skeleton, which is a natural anticancer drug extracted from the bark of Pacific yew (Taxus brevifolia) for the first time (Wani et al., 1971). It binds to tubulin, promotes its polymerization, inhibits the mitosis of cancer cells, and prevents the proliferation of cancer cells (Kelling et al., 2003; She Kun et al., 2001). In 1992, it was officially approved by the US FDA as a new anticancer drug. Breast cancer and lung cancer have a better effect. Studies in recent years have shown that paclitaxel also has certain potential in the treatment of other diseases, such as malignant melanoma (Roshid et al., 1994), rheumatoid arthritis (Oliver et al., 199...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P17/02C07D305/14
Inventor 付玉杰祖元刚李双明孙蕊骆浩张然李庆勇张颜滨
Owner NORTHEAST FORESTRY UNIVERSITY
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