Method for extracting four-taxone compounds with high-efficiency abduction

A high-efficiency paclitaxel technology, applied in organic chemistry, fermentation, etc., can solve the problems of weak environmental adaptability, no value of fermentation production, extraction of paclitaxel, etc., and achieve large-scale industrial production. The method is simple and easy to improve Yield effect
CN101220384AInactive Publication Date: 2008-07-16NORTHEAST FORESTRY UNIVERSITY +1
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Patent Information

Authority / Receiving Office
CN Β· China
Patent Type
Applications(China)
Current Assignee / Owner
NORTHEAST FORESTRY UNIVERSITY
Publication Date
2008-07-16
Estimated Expiration
Not applicable Β· inactive patent

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Abstract

The invention relates to a method for utilizing enzyme, ultraviolet radiation and oxygen as inductors for the high efficient production of 10-deacetylbaccatin III, baccatin III, cephalomannine and paclitaxel in taxus chinensis branches and leaves by the method of fresh grinding homogenization. The invention takes the fresh branches and leaves of taxus chinensis as raw materials for fresh grinding homogenization, then the enzyme is added, the oxygen is introduced, and a solid is treated with 80 percent ethanol ultrasonic extraction under ultraviolet irradiation. The contents of the 10-deacetylbaccatin III, the baccatin III, the cephalomannine and the paclitaxel in the branches and leaves of the taxus chinensis can be averagely improved by more than 17 percent, 23 percent, 74 percent and 23 percent respectively by the biological induction method. The raw materials used by the invention are the renewable fresh branches and leaves of the taxus chinensis, which can not only ensure no damage to ecological resources, but also can extract the effective components of taxus chinensis and the biological semisynthetic precursor substances with high efficiency. Furthermore, the method has simple and easy operation, which is applicable to industrial application and has great significance for industrial production.
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Description

technical field

[0001] The invention relates to a method for efficiently inducing and increasing the content of paclitaxel and its semi-synthetic precursors in the fresh branches and leaves of Taxus chinensis. Background technique

[0002] Paclitaxel (paclitaxel, figure 1 ) is a diterpenoid compound with a pentamethylpentadecene skeleton, which is a natural anticancer drug extracted from the bark of Pacific yew (Taxus brevifolia) for the first time (Wani et al., 1971). It binds to tubulin, promotes its polymerization, inhibits the mitosis of cancer cells, and prevents the proliferation of cancer cells (Kelling et al., 2003; She Kun et al., 2001). In 1992, it was officially approved by the US FDA as a new anticancer drug. Breast cancer and lung cancer have a better effect. Studies in recent years have shown that paclitaxel also has certain potential in the treatment of other diseases, such as malignant melanoma (Roshid et al., 1994), rheumatoid arthritis (Oliver et al., 199...

Claims

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