Technique for extracting wheatgrass protein polypeptide
A protein polypeptide and extraction process technology, applied in the field of wheatgrass protein polypeptide extraction process, can solve the problems of poor functional properties, grassy taste, and low protein utilization rate of the final product, so as to solve the problems of solubility and grassy taste, and improve protein The effect of utilization
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[0022] Example 1
[0023] The first step: pulverize the wheatgrass, then add 6 kg of 90℃ hot water per kilogram of the pulverized wheatgrass, and extract at 95℃ for 1 hour. After the extraction, the temperature will be reduced to about 40-50℃ to obtain the original Slurry
[0024] Step 2: Adjust the pH of the original slurry to 6.0, add 0.3% cellulase to the original slurry, and enzymatically hydrolyze at 55°C for 7.0 hours;
[0025] The third step: adjust the pH of the above-mentioned original slurry after the second step of enzymatic hydrolysis to 7.5, then add the mixed enzyme, the alkaline protease in the mixed enzyme: composite protease (U / U) = 1:5, at a temperature of 55°C Enzymatic hydrolysis for 8 hours, and then heat at 100°C for 5 minutes to terminate the enzymatic hydrolysis reaction;
[0026] Step 4: Then centrifuge the enzymatic hydrolysate and centrifuge for 30 minutes under the condition of a centrifugal force of 4000×g to obtain a supernatant with a degree of hydro...
Example Embodiment
[0029] Example 2
[0030] The first step: pulverize wheatgrass, then add 8kg of 85℃ hot water to each kilogram of wheatgrass grind, and extract at 90℃ for 1.5 hours. After extraction, the temperature will be reduced to about 40-50℃ to obtain the original Slurry
[0031] The second step: adjust the pH of the original slurry to 6.5, add 0.5% cellulase to the original slurry, and enzymatically hydrolyze at 50°C for 6.5 hours;
[0032] The third step: adjust the pH of the above-mentioned original slurry after the second step of enzymatic hydrolysis to 6.6, and then add the mixed enzyme, the alkaline protease in the mixed enzyme: papain (U / U) = 2:7, at a temperature of 50°C Enzymatic hydrolysis for 10 hours, and then heat at 95°C for 10 minutes to terminate the enzymatic hydrolysis reaction;
[0033] Step 4: Then centrifuge the enzymatic hydrolysate and centrifuge for 25 minutes under the condition of a centrifugal force of 5000×g to obtain a supernatant with a hydrolysis degree of 5% ...
Example Embodiment
[0036] Example 3
[0037] The first step: pulverize the wheat grass, then add 10 kg of hot water at 85°C per kilogram of the pulverized wheat grass, and extract at 85°C for 2 hours. After the extraction, the temperature is reduced to about 40-50°C to obtain the original Slurry
[0038] Step 2: Adjust the pH of the original slurry to 7.0, add 0.5% cellulase to the original slurry, and enzymatically hydrolyze at 40°C for 7.0 hours;
[0039] The third step: adjust the pH of the above-mentioned original slurry after the second step of enzymatic hydrolysis to 7.0, and then add the mixed enzyme, the alkaline protease in the mixed enzyme: papain (U / U) = 1:7, and the temperature is 40℃ After enzymatic hydrolysis for 12 hours, the enzymatic hydrolysis reaction was terminated after heating at 95°C for 10 minutes;
[0040] Step 4: Then centrifuge the enzymatic hydrolysate and centrifuge for 20 minutes under the condition of a centrifugal force of 6000×g to obtain a supernatant with a hydroly...
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