Technique for extracting wheatgrass protein polypeptide

A protein polypeptide and extraction process technology, applied in the field of wheatgrass protein polypeptide extraction process, can solve the problems of poor functional properties, grassy taste, and low protein utilization rate of the final product, so as to solve the problems of solubility and grassy taste, and improve protein The effect of utilization

Inactive Publication Date: 2008-07-16

SOUTH CHINA UNIV OF TECH

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AI-Extracted Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to overcome the defects of the prior art such as low protein utilization rate, po...

Abstract

The invention pertains to the field of food biotechnology, which more particularly relates to a wheat grass protein polypeptide extraction technique which includes the following steps that: (1) the wheat grass is smashed and then hot water is added for extraction so as to obtain original slurry; (2) the pH of the original slurry is regulated to 6.0 to 7.0, cellulase is firstly added in the original slurry for enzymolysis, then the pH value is regulated to 6.6 to 7.5, and mixed enzyme is added for enzymolysis, then the enzymolysis reaction is terminated to obtain the enzymolysis liquid; (3) then the enzymolysis liquid is treated with centrifugal separation to obtain the supernatant liquor which is treated with decompression concentration and freeze drying to obtain the wheat grass protein polypeptide powder. The invention adopts a biological enzymolysis method for the extraction and preparation of wheat grass protein and adopts a two-step enzymolysis method; the cellulase is firstly adopted to damage the cell walls and other fibrous materials in the wheat grass, which is conductive to the dissolution and the action of the protein, thus the invention can not only improve the utilization ratio of the protein to lead the utilization ratio of the protein to achieve more than 90 percent, but also can solve the solubility and the grass taste problems of a traditional extraction technology.

Application Domain

Peptide preparation methodsFermentation

Technology Topic

Food biotechnologyChemistry +11

Examples

Experimental program(4)

Example Embodiment

[0022] Example 1

[0023] The first step: pulverize the wheatgrass, then add 6 kg of 90℃ hot water per kilogram of the pulverized wheatgrass, and extract at 95℃ for 1 hour. After the extraction, the temperature will be reduced to about 40-50℃ to obtain the original Slurry

[0024] Step 2: Adjust the pH of the original slurry to 6.0, add 0.3% cellulase to the original slurry, and enzymatically hydrolyze at 55°C for 7.0 hours;

[0025] The third step: adjust the pH of the above-mentioned original slurry after the second step of enzymatic hydrolysis to 7.5, then add the mixed enzyme, the alkaline protease in the mixed enzyme: composite protease (U/U) = 1:5, at a temperature of 55°C Enzymatic hydrolysis for 8 hours, and then heat at 100°C for 5 minutes to terminate the enzymatic hydrolysis reaction;

[0026] Step 4: Then centrifuge the enzymatic hydrolysate and centrifuge for 30 minutes under the condition of a centrifugal force of 4000×g to obtain a supernatant with a degree of hydrolysis of 10% and a protein utilization rate of 91%;

[0027] The fifth step: the supernatant obtained in the fourth step above, under the condition that the vacuum degree is 0.1Pa, the temperature is 50°C, and the solid content is concentrated to 40% under reduced pressure;

[0028] The sixth step: freeze-dry the concentrated liquid obtained in the fifth step above to obtain wheatgrass protein polypeptide powder.

Example Embodiment

[0029] Example 2

[0030] The first step: pulverize wheatgrass, then add 8kg of 85℃ hot water to each kilogram of wheatgrass grind, and extract at 90℃ for 1.5 hours. After extraction, the temperature will be reduced to about 40-50℃ to obtain the original Slurry

[0031] The second step: adjust the pH of the original slurry to 6.5, add 0.5% cellulase to the original slurry, and enzymatically hydrolyze at 50°C for 6.5 hours;

[0032] The third step: adjust the pH of the above-mentioned original slurry after the second step of enzymatic hydrolysis to 6.6, and then add the mixed enzyme, the alkaline protease in the mixed enzyme: papain (U/U) = 2:7, at a temperature of 50°C Enzymatic hydrolysis for 10 hours, and then heat at 95°C for 10 minutes to terminate the enzymatic hydrolysis reaction;

[0033] Step 4: Then centrifuge the enzymatic hydrolysate and centrifuge for 25 minutes under the condition of a centrifugal force of 5000×g to obtain a supernatant with a hydrolysis degree of 5% and a protein utilization rate of 87%;

[0034] The fifth step: the supernatant obtained in the fourth step above, under the condition that the vacuum degree is 0.09 Pa, the temperature is 60°C, and the solid content is concentrated to 20% under reduced pressure;

[0035] The sixth step: freeze-dry the concentrated liquid obtained in the fifth step above to obtain wheatgrass protein polypeptide powder.

Example Embodiment

[0036] Example 3

[0037] The first step: pulverize the wheat grass, then add 10 kg of hot water at 85°C per kilogram of the pulverized wheat grass, and extract at 85°C for 2 hours. After the extraction, the temperature is reduced to about 40-50°C to obtain the original Slurry

[0038] Step 2: Adjust the pH of the original slurry to 7.0, add 0.5% cellulase to the original slurry, and enzymatically hydrolyze at 40°C for 7.0 hours;

[0039] The third step: adjust the pH of the above-mentioned original slurry after the second step of enzymatic hydrolysis to 7.0, and then add the mixed enzyme, the alkaline protease in the mixed enzyme: papain (U/U) = 1:7, and the temperature is 40℃ After enzymatic hydrolysis for 12 hours, the enzymatic hydrolysis reaction was terminated after heating at 95°C for 10 minutes;

[0040] Step 4: Then centrifuge the enzymatic hydrolysate and centrifuge for 20 minutes under the condition of a centrifugal force of 6000×g to obtain a supernatant with a hydrolysis degree of 7% and a protein utilization rate of 85%;

[0041] Step 5: Concentrate the supernatant obtained in the above step 4 with a vacuum of 0.095 Pa and a temperature of 55°C to 35% of solid content under reduced pressure;

[0042] The sixth step: freeze-dry the concentrated liquid obtained in the fifth step above to obtain wheatgrass protein polypeptide powder.

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Classification and recommendation of technical efficacy words

Improve protein utilization

Technique for extracting wheatgrass protein polypeptide

AI-Extracted Technical Summary

Problems solved by technology

Abstract

Examples

Example Embodiment

Example Embodiment

Example Embodiment

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Description & Claims & Application Information

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