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Technique for extracting wheatgrass protein polypeptide

A protein polypeptide and extraction process technology, applied in the field of wheatgrass protein polypeptide extraction process, can solve the problems of poor functional properties, grassy taste, and low protein utilization rate of the final product, so as to solve the problems of solubility and grassy taste, and improve protein The effect of utilization

Inactive Publication Date: 2008-07-16
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to overcome the defects of the prior art such as low protein utilization rate, poor functional properties of the final product, and grassy taste, and provide a wheatgrass protein polypeptide extraction process

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Step 1: Grind wheatgrass, then add 6kg of 90°C hot water to each kilogram of wheatgrass crushed material, extract at 95°C for 1 hour, cool down to about 40-50°C after extraction, and obtain the original slurry;

[0024] Step 2: adjust the pH of the original slurry to 6.0, add 0.3% cellulase to the original slurry, and enzymolyze it at 55°C for 7.0 hours;

[0025] The third step: adjust the pH of the above-mentioned raw slurry liquid that has been enzymatically hydrolyzed in the second step to 7.5, and then add mixed enzymes. Enzymolysis at low temperature for 8 hours, and then heating at 100°C for 5 minutes to terminate the enzymolysis reaction;

[0026] Step 4: Then centrifuge the enzymatic hydrolyzate, and centrifuge for 30 minutes under the condition of centrifugal force of 4000×g to obtain supernatant, the degree of hydrolysis reaches 10%, and the utilization rate of protein reaches 91%;

[0027] The fifth step: Concentrate the supernatant obtained in the above fo...

Embodiment 2

[0030] Step 1: Grind wheatgrass, then add 8kg of hot water at 85°C to each kilogram of wheatgrass crushed material, extract at 90°C for 1.5 hours, cool down to about 40-50°C after extraction, and obtain the original slurry;

[0031] Step 2: adjust the pH of the original slurry to 6.5, add 0.5% cellulase to the original slurry, and perform enzymatic hydrolysis at 50°C for 6.5 hours;

[0032] The third step: adjust the pH of the above-mentioned raw slurry liquid that has been enzymatically hydrolyzed in the second step to 6.6, and then add the mixed enzyme, the alkaline protease in the mixed enzyme: papain (U / U) = 2: 7, at a temperature of 50 ° C Enzymolysis at low temperature for 10 hours, then heating at 95°C for 10 minutes to terminate the enzymolysis reaction;

[0033] Step 4: Then centrifuge the enzymatic hydrolyzate and centrifuge for 25 minutes under the condition of centrifugal force of 5000×g to obtain a supernatant with a degree of hydrolysis of 5% and a protein utili...

Embodiment 3

[0037] Step 1: Grind wheatgrass, then add 10kg of 85°C hot water to each kilogram of wheatgrass crushed material, extract at 85°C for 2 hours, cool down to about 40-50°C after extraction, and obtain the original slurry;

[0038] Step 2: adjust the pH of the original slurry to 7.0, add 0.5% cellulase to the original slurry, and enzymolyze it at 40°C for 7.0 hours;

[0039] The third step: adjust the pH of the above-mentioned raw slurry liquid that has been enzymolyzed in the second step to 7.0, and then add mixed enzymes, alkaline protease in mixed enzymes: papain (U / U) = 1: 7, at 40 ° C Enzymolysis at low temperature for 12 hours, and then heating at 95°C for 10 minutes to terminate the enzymolysis reaction;

[0040] Step 4: Then centrifuge the enzymatic hydrolyzate, and centrifuge for 20 minutes under the condition of centrifugal force of 6000×g to obtain a supernatant with a degree of hydrolysis of 7% and a protein utilization rate of 85%;

[0041] The fifth step: Concentr...

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Abstract

The invention pertains to the field of food biotechnology, which more particularly relates to a wheat grass protein polypeptide extraction technique which includes the following steps that: (1) the wheat grass is smashed and then hot water is added for extraction so as to obtain original slurry; (2) the pH of the original slurry is regulated to 6.0 to 7.0, cellulase is firstly added in the original slurry for enzymolysis, then the pH value is regulated to 6.6 to 7.5, and mixed enzyme is added for enzymolysis, then the enzymolysis reaction is terminated to obtain the enzymolysis liquid; (3) then the enzymolysis liquid is treated with centrifugal separation to obtain the supernatant liquor which is treated with decompression concentration and freeze drying to obtain the wheat grass protein polypeptide powder. The invention adopts a biological enzymolysis method for the extraction and preparation of wheat grass protein and adopts a two-step enzymolysis method; the cellulase is firstly adopted to damage the cell walls and other fibrous materials in the wheat grass, which is conductive to the dissolution and the action of the protein, thus the invention can not only improve the utilization ratio of the protein to lead the utilization ratio of the protein to achieve more than 90 percent, but also can solve the solubility and the grass taste problems of a traditional extraction technology.

Description

technical field [0001] The invention belongs to the field of food biotechnology, in particular to a wheatgrass protein polypeptide extraction process Background technique [0002] Protein is one of the basic substances that constitute plant cells. During the growth of plants, there is continuous protein synthesis in the stem and leaf cells above the ground to meet the needs of building new cell tissues and organs. This part of the protein is called leaf protein ( Leaves Protein Concentrates referred to as LPC), they belong to functional proteins. Because it exists in plant stems and leaves, it is the largest renewable protein resource. Leaf protein is a kind of polymeric substance, containing 17 or 18 kinds of amino acids, including 8 kinds of amino acids necessary for the human body, and also contains vitamins, but does not contain cholesterol. Green plant leaves are the largest renewable protein resource on the earth. The leaf protein extracted from green leaves has high...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K1/14
Inventor 王炜安小宁
Owner SOUTH CHINA UNIV OF TECH
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