Detecting reagent kit and detecting method for sulphonamides multi-relict in animal derived food

Abstract

The invention pertains to the technical field of food detection and analysis, in particular to a multi-residue detection kit of sulfonamides in animal derived food and the detection method thereof, the kit is composed of a box body, a coated plate and a bracket, the coated plate and the bracket are fixedly arranged in the box body, wherein, (1) a concave hole of the standard solution of sulfonamides, (2) the concave hole of antigen working solution of enzyme labeled sulfonamides, (3) the concave hole of antigen dilute solution of enzyme labeled sulfonamides, (4) the concave hole of substrate chromogenic agent A solution, (5) the concave hole of substrate chromogenic agent B solution, (6) the concave hole of stop solution, and (7) the concave hole of concentrated washing liquid are arranged on the bracket. The invention is characterized by specificity, sensitiveness, accuracy, high speed, convenience, low cost and so on; furthermore, the scope of application is broad, the detection efficiency is high, the operation is simple, and the invention has both economic benefits and social benefits.

Description

technical field [0001] The invention belongs to the technical field of food detection and analysis, in particular to a multi-residue detection kit of sulfa drugs in animal-derived food and a detection method thereof. Background technique [0002] The basic structure of sulfa drugs is sulfonamide, which has aromatic amino group and sulfonamide group, and most of them are amphoteric compounds, and the drugs have certain acidity. The antibacterial effect of sulfonamides is due to the fact that sulfanilamide can be decomposed from sulfa drugs, and its molecular size and shape are similar to those of p-aminobenzoic acid in folic acid, and its chemical properties are also similar. Due to the lack of selectivity of bacteria to the two, a large amount of sulfanilamide is absorbed by bacteria instead of p-aminobenzoic acid, which interferes with the synthesis of bacterial folic acid and affects its growth and reproduction, thereby inhibiting most Gram-positive bacteria and some Nega...

AI Technical Summary

Problems solved by technology

Due to the complicated equipment and cumbersome pretreatment process, the instrumental analysis method is not suitable for on-site monitoring and screening of a large number of samples. Among them, the ELISA method has been used as a new screening method for sulfa drug residues in animal-derived foods, such as China Patent application (200510086777), although this method has the characteristics of quickness and simplicity compared with instrumental analysis technology, what it adopts is monoclonal antibody and secondary antibody enzyme technology and indirect competitive immunoassay method. Anti-enzyme and primary antibody binding reaction, site crossover problem, easy to cause non-specific binding, resulting in binding deviation and poor sensitivity, while the indirect competitive immunoassay method has poor stability

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