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New method for remains nuclear DNA individual identification

An identification method and individual technology, applied in the field of identification, can solve problems such as destruction and identification difficulties, and achieve the effect of simple operation steps, easy to master, and reducing the loss of DNA molecules

Inactive Publication Date: 2012-09-12
XI AN JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0055] In the field of forensic science applications, the murder, mutilation, unidentified corpse, or fire, explosion, plane crash and other major accidents caused by many deaths, corpse damage, personal physical characteristics (face, fingerprints, clothing, etc.) and body soft tissue integrity Destroyed, only hard tissues such as bones and teeth are left behind, which makes it difficult to identify. The purpose of the present invention is to use the consistency theory of genetic markers of bone and tooth genomic DNA and blood DNA, and the stability of nuclear DNA in bone and tooth tissues and a high degree of polymorphism, a new method for individual identification of nuclear DNA of remains by bone and tooth tissue nuclear DNA typing is proposed, which opens up a new solution for the identification of remains

Method used

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  • New method for remains nuclear DNA individual identification
  • New method for remains nuclear DNA individual identification
  • New method for remains nuclear DNA individual identification

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0101] Example 1: Using this method to compare the DNA extraction and typing efficiency of bone and dental nucleus in different environments and storage times.

[0102] 5 groups of 86 cases of human remains samples from 2 to 60 years were extracted for nuclear DNA, and the PM Loci Kit was used for simultaneous amplification and reverse dot hybridization DNA typing of five loci.

[0103] Table 1 Preservation and grouping of remains samples

[0104]

[0105] The results show that the method can obtain good extraction quality and typing efficiency of nuclear DNA from remains in different preservation conditions.

[0106] Table 2 The proportion of successful nuclear DNA typing and the quality of DNA extraction

[0107]

Embodiment 2

[0108] Example 2: The Bureau of Beilin District of Xi'an City provides a case of mutilation of a corpse that occurred in Beilin District on January 8, 1991. After investigation, the sons of Zhang and Cai disappeared during this period. Entrusted by Xi'an Beilin District Public Security Bureau, it is required to carry out personal identification of the source of the corpse. Due to the complete corruption of the soft tissue, the sample material was sent to a dismembered femur for examination. 1 mL of Zhang's and Cai's venous blood were anticoagulated with EDTA. This method was used to extract bone nucleus DNA, and PM Loci Kit was used for individual identification and genotyping. The results are shown in Table 3:

[0109] Table 3 Preservation and grouping of remains samples

[0110]

[0111] The results showed: PI total = 6842229.54; RCP = 99.99%. The probability that the remains are the sons of Zhang and Cai is 99.99%.

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Abstract

The invention discloses a new method of identifying remain (bones, teeth) nuclear DNA individuals. The principle is that: the silicon dioxide fine particles and the nuclear DNA molecules are combined specially at the situation of guanidinium thiocynate, meanwhile, decalcification and proteopepsis are processed on the combination by utilizing EDTA and PK, the loss of DNA molecules are reduced at amaximum degree and the aim of separating nucleic acid at a highest efficiency is achieved. Compared with the organic solvent method, the salting-out method and the Chelex-100 method, the new method of identifying remain (bones, teeth) nuclear DNA individuals has the advantages of high DNA extraction efficiency and high quality with using the same amount of bone powder, the operation is simple andeasy to control, and the new method of identifying remain (bones, teeth) nuclear DNA individuals does not need large scale or special equipment; meanwhile, the process of extracting bone and teeth DNA takes only one day; furthermore, the new method of identifying remain (bones, teeth) nuclear DNA individuals has favorable reproducibility, blind test inspection is processed on one sample, the results shows high consistency.

Description

technical field [0001] The invention relates to an identification method, especially a new method for individual identification of nuclear DNA of remains. The method can obtain high-quality nuclear DNA, and solves the problem of individual identification, gender identification, and paternity identification of remains after the body is damaged, so as to provide Provide scientific basis for judicial detection, trial, insurance, etc. Background technique [0002] Murder, mutilation, unidentified corpse, or fire, explosion, plane crash and other major accidents caused multiple deaths, corpse damage, personal physical characteristics (face, fingerprints, clothing, etc.) and body soft tissue are completely destroyed, leaving only bones and teeth And other hard tissue. The traditional individual identification methods to identify mutilated corpses, anonymous corpses, ossified corpses and other damaged corpses mostly rely on the inference of residual skin texture, bone morphology a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68
Inventor 李生斌托娅郑海波李双顶巩五虎
Owner XI AN JIAOTONG UNIV