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Pyridine degradation bacterium strain and uses thereof

A technology for degrading pyridine and sewage, applied in the field of pyridine-degrading bacteria, can solve problems such as no reports of pyridine degradation, and achieve the effects of enhanced impact resistance, broad application prospects, and high degradation efficiency

Inactive Publication Date: 2008-09-03
PEKING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] There are few international studies on Shinella zoogloeoides, and there is no report on its degradation of pyridine

Method used

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  • Pyridine degradation bacterium strain and uses thereof
  • Pyridine degradation bacterium strain and uses thereof
  • Pyridine degradation bacterium strain and uses thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1. Isolation, purification and identification of Shinella zoogloeoides BC026

[0028] 1. Isolation and purification of Shinella zoogloeoides BC026

[0029] Shinella zoogloeoides BC026 is a Gram-negative bacterium obtained by sampling from the activated sludge in the secondary sedimentation tank of the sewage treatment system of Shougang Coking Plant, after domestication, isolation and purification. Specific steps are as follows:

[0030] Take the return sludge from the secondary settling tank of the wastewater treatment system of Shougang Coking Plant. Add a small amount of pyridine solution (keep the concentration of pyridine at 100-200mg / L) dropwise in the container containing the sludge sample, aerate intermittently, add 10mL of pyridine (300mg / L) and 50mL of the original coking wastewater, and add 2mL of phosphoric acid at the same time Salt buffer (8.5g KH per L 2 PO 4 , 21.75gK 2 HPO 4 , 33.4gNa 2 HPO 4 ·7H 2 O, 1.7gNH 4 Cl, pH 7.2) and 2mL trace...

Embodiment 2

[0051] Example 2, detection of the biodegradation effect of Shinella zoogloeoides BC026 CGMCC №.2224 on pyridine

[0052] 1. Detection of pyridine biodegradation ability of Shinella zoogloeoides BC026 CGMCC №.2224

[0053] Under the condition of using pyridine as the only carbon and nitrogen source, the degradation ability of BC026 to pyridine was investigated. It was found that BC026 bacteria had a good removal effect on pyridine and could complete the degradation in a short time. Specific steps are as follows:

[0054] 1) Take 2 mL of the LB culture solution of Shinella zoogloeoides BC026 CGMCC №.2224 cultivated to the logarithmic phase, collect the bacterial precipitate, inoculate into a Erlenmeyer flask containing 200 mL of inorganic salt medium, and cultivate to the logarithmic phase.

[0055] 2) Centrifuge the bacterial liquid, resuspend the obtained Shinella zoogloeoides BC026 cell pellet with inorganic salt medium, centrifuge three times to remove the impurities adso...

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Abstract

The invention discloses a pyridine degradable bacteria and application thereof. The pyridine degradable bacteria are Shinella zoogloeoides BC026 CGMCC No.2224. Applying the inventive Shinella zoogloeoides BC026 in decomposition of pyridine in drain system, not only high decomposition efficiency is obtained, but also the inventive Shinella zoogloeoides BC026 has characteristic of distillage recycle, can exert important function in drain processing, impact endurance of the system is enhanced.

Description

technical field [0001] The invention relates to a strain of pyridine degrading bacteria and its application. Background technique [0002] Pyridine is a colorless liquid with a pungent odor at room temperature, and is currently one of the most widely developed and applied heterocyclic aromatic hydrocarbons. Pyridine is toxic, carcinogenic, teratogenic, mutagenic, and easy to accumulate in organisms. It can be harmful to the human body through inhalation, ingestion or skin contact. It can anesthetize the central nervous system and stimulate the eyes and upper respiratory tract. Long-term inhalation can lead to dizziness, headache, insomnia, unsteady gait and digestive tract dysfunction, liver and kidney damage, and dermatitis. Pyridine is a good solvent, it can be used as a denaturant, a dyeing aid, and a series of other products in industry, including the synthesis of pesticides, medicines, dyes, daily chemicals, spices, feed additives, rubber additives, etc. original thi...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C02F101/38
Inventor 温东辉孙庆华柏耀辉赵翠唐孝炎
Owner PEKING UNIV
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