Unlock instant, AI-driven research and patent intelligence for your innovation.

Method for separating 24-dehydrogenation cholesterol and cholesterol

A technology for dehydrocholesterol and cholesterol, applied in the preparation of steroids, chemical instruments and methods, steroids, etc., can solve the problems of low content and yield, cumbersome process, long production cycle, etc. Good separation effect and stable operation

Active Publication Date: 2008-09-24
ZHEJIANG UNIV +1
View PDF2 Cites 15 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The first method obtains 24-dehydrocholesterol by column chromatography, its content and yield are not high, and the separation efficiency is low
The second method has good selectivity, but the production cost is relatively large, the process is relatively cumbersome, and the production cycle is long
The third method has a higher yield, but the production cycle is longer, and more acid-base solvents are used, which is easy to pollute the environment

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] The mixture of 24-dehydrocholesterol and cholesterol (24-dehydrocholesterol / cholesterol=13.64%, mass ratio) is dissolved in petroleum ether with a boiling range of 60 to 90°C, and the total concentration of 24-dehydrocholesterol and cholesterol is 0.9 g / L of raw material solution. The mixture of methanol and sulfolane with a percentage of sulfolane accounting for 70% of the total volume is used as the extractant, petroleum ether with a boiling range of 60-90° C. is used as the detergent, and the flow ratio of the extractant, detergent, and raw material solution is 6: 4.5:1.5, carry out fractional distillation and extraction at 50°C, collect the extract and raffinate, and concentrate in vacuo to obtain 24-dehydrocholesterol with a purity of 93.83%, a yield of 70.0%, and cholesterol with a purity of 97.86%. The yield was 95.0%.

Embodiment 2

[0021] The raw materials (24-dehydrocholesterol / cholesterol=12.98%, mass ratio) are dissolved in petroleum ether with a boiling range of 60-90° C. to prepare a raw material solution with a total concentration of 24-dehydrocholesterol and cholesterol of 1.8 g / L. The mixture of methanol and sulfolane with a percentage of sulfolane accounting for 70% of the total volume is used as the extractant, petroleum ether with a boiling range of 60-90° C. is used as the detergent, and the flow ratio of the extractant, detergent, and raw material solution is 6: 4.5:0.3, carry out fractional distillation and extraction at 50°C, collect the extract and raffinate, and concentrate in vacuo to obtain 24-dehydrocholesterol with a purity of 96.64%. The yield is 80.0%, and the purity is 98.29%. The yield was 98.5%.

Embodiment 3

[0023] The raw material (24-dehydrocholesterol / cholesterol=13.64%, mass ratio) was dissolved in octane to prepare a raw material solution with a total concentration of 24-dehydrocholesterol and cholesterol of 1.8 g / L. With the mixture of methanol and sulfolane with sulfolane accounting for 50% of the total volume as the extractant, octane as the detergent, the flow ratio of the extractant, detergent, and raw material liquid is 6:3.5:1.5, at 40 ° C Fractional distillation and extraction were carried out, the extract and raffinate were collected, and concentrated in vacuo to obtain 24-dehydrocholesterol with a purity of 85.56%, with a yield of 85.0%, and cholesterol with a purity of 92.10%, with a yield of 99.5%.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present invention relates to a method for separating 24-dehydrogenated cholesterol and cholesterol. Mixture containing 24-dehydrogenated cholesterol and cholesterol is resolved in solvent to prepare raw material liquid, which is treated by fractional extraction, extraction liquid is collected and condensed to obtain 24-dehydrogenated cholesterol, and residual extraction liquid is collected and condensed to obtain cholesterol; the extraction agent for fractional extraction is one or mixture of methanol, octanol and sulfolane; just like the fractionally extracted washing agent, the solvent is one of sherwood oil, hexane and octane. The process applies industrial organic solvent, the phase-separating speed is high, and the method is characterized in high productivity, low energy consumption, simple process, stable operation, environmental protection, high product quality and low cost and is suitable for industrialized production.

Description

technical field [0001] The invention relates to a method for separating 24-dehydrocholesterol and cholesterol, in particular to a method for adopting a fractional distillation extraction method for 24-dehydrocholesterol and cholesterol. Background technique [0002] Cholesterol, also known as cholesterol, is a derivative of cyclopentane polyhydrophenanthrene. Cholesterol is widely present in animals, especially in brain and nerve tissue, and is also high in kidney, spleen, skin, liver and bile. Cholesterol is an indispensable and important substance in animal tissue cells. It not only participates in the formation of cell membranes, but also is a raw material for the synthesis of bile acids, vitamin D and steroid hormones. 24-Dehydrocholesterol (Desmosterol) is a sterol substance extracted from mixed phytosterols, animal serum, liver and some other animal tissues, and is the direct precursor of cholesterol biosynthesis. Studies at home and abroad have proved that 24-dehydr...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C07J9/00C07J75/00
Inventor 任其龙苏宝根邢华斌杨亦文陈蔚
Owner ZHEJIANG UNIV