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Microorganism cause concrete or concrete, producing method and application thereof

A microbial cement and production method technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problem of difficult to achieve high-quality ancient building protection and restoration, failure to achieve strength and size, and organic polymer weather resistance sex is difficult to guarantee and other issues

Inactive Publication Date: 2008-09-24
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, organic polymers have problems such as difficult to guarantee weather resistance, poor water permeability and air permeability, and it is difficult to achieve the purpose of high-quality ancient building protection and restoration.
[0004] The U.S. patent "Bacteriogenic mineral plugging" (application number: 5,143,155) discloses the use of mineral crystals formed by microorganisms to reduce the permeability of underground formations to help oil fields increase production or control (reduce) aquifers The outflow of pollutants, but the amount and intensity of mineral precipitation formed by this method are low, which cannot meet the needs of civil engineering applications
[0005] The patent "method for preparing calcium carbonate by microbial deposition" (patent number: ZL200510094744.5) of Southeast University discloses a method for preparing calcium carbonate by microbial deposition. The calcium carbonate obtained by the method is granular, and its microstructure is spherical calcite crystals. Used as a building filler, but lacks the strength and size required for engineering applications

Method used

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  • Microorganism cause concrete or concrete, producing method and application thereof
  • Microorganism cause concrete or concrete, producing method and application thereof
  • Microorganism cause concrete or concrete, producing method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0070] Preparation of Microbiologically Originated Cement

[0071] (1) Weigh 15.75g of tris, dilute to 1L with deionized water, titrate to pH=8 with 1M HCl, add 20g of yeast powder, (NH 4 ) 2 SO 4 10g, stir evenly to obtain culture medium;

[0072] (2) Sporosarcina pasteurii seed strain ATCC11859 (purchased from the American Culture Collection Center) was resuscitated and divided into plastic ampoules, and one tube (1.8ml) was inoculated into 250ml prepared in step (1). In the culture medium, shake culture at 30°C and 150rpm for 40h to obtain the bacterial solution;

[0073] (3) Mix 180g of urea, 200g of calcium chloride and 300g of calcium nitrate tetrahydrate, add water to 1L, and stir until fully dissolved to obtain a compound solution;

[0074] (4) Titrate the bacterial solution cultivated in step (2) to pH=7 with 1M HCl, take 30ml of bacterial solution and 20ml of compounding solution, mix evenly, and let stand at 28°C for 48h, the result (see figure 1 ) The cementit...

Embodiment 2

[0078] Preparation of Concrete of Microbial Origin

[0079] Titrate the bacterial solution cultured in step (2) of Example 1 to pH=7 with 1M HCl, take 3ml into a test tube, then take 3ml of the compound solution prepared in Example 1 into the test tube, mix well, and react at 28°C 1h, then add 3ml of sand (particle size 0.63-1.25mm), let stand at 28°C for 48h, the result (see Figure 4 ) At the bottom of the test tube, it can be seen that the sand particles in contact with the microbial cement are bonded by the microbial cement, and the bonded product is the microbial concrete.

Embodiment 3

[0081] Preparation of Concrete of Microbial Origin

[0082] Add 10ml of sand grains (particle size 1.25-2.5mm) in a 150ml container, titrate the bacterial solution prepared in Example 1 to pH=7 with 1M HCl, take 30ml into the container, and then take 40ml of the compounding solution prepared in Example 1 into the container , mixed evenly, and stood at 28°C for 48 hours. As a result, there was microbial cement at the bottom of the container (on top of the sand grains), and a layer of sand grains in contact with the microbial cement was bonded by the microbial cement. The product of microbial origin is "concrete".

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Abstract

The invention discloses a microbiogenic cement and concrete and a production method thereof, which mainly mixes the Bacillus pasteurii solution for producing urease and an admixture of containing urea and mineral calcium salt and obtains the microbiogenic cement after reacting in a certain condition; adds admixture such as sand etc. into the mixture of mixing the bacterial solution and the mixture and reacts to generate the microbiogenic concrete. The microbiogenic cement or concrete of the invention can be used on protection and restoration of masonry ancient architectures and repair of cracks on concrete building or treatment of liquescent sandy soil. The invention provides a permanent method of safety, effectiveness and without environmental hazard for repair and strengthening technology of large quantity of civil architecture and structure; the invention has the advantages of the good effects, the good weatherability, the good permeability of gas and the good permeability of water and being especially suitable for treatment of the sandy soil; the invention is safe and environmental-friendly, and belongs to the environmental-friendly restoring material.

Description

technical field [0001] The invention belongs to biological building materials, and in particular relates to a microbial-genetic cement or concrete and a production method thereof. The invention can be used for protection and restoration of masonry ancient buildings, repair of cracks in concrete buildings, treatment of liquefied sand foundations and the like. Background technique [0002] The ancient masonry buildings with a history of thousands of years in our country are mostly made of limestone, dolomite, marble and clay bricks. These masonry materials are relatively rigid and have the characteristics of hard and brittle; and the structure itself has many pores and a large contact surface area with the environment, so it is very easy to be corroded and polluted, which reduces the original force capacity of the building. Under the action of external force or its own gravity, it is extremely easy to crack and deform, causing the building to be damaged. In the past, enginee...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P3/00C12N1/20C04B28/00C04B41/45C04B14/06C04B14/26C12R1/07
Inventor 程晓辉沈吉云
Owner TSINGHUA UNIV
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