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Method for preparing chitosan oligosaccharide with trichoderma reesei cellulase

A cellulase, Trichoderma reesei technology, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of difficult control, wide distribution, high cost, reduce costs, avoid damage, The effect of mild reaction conditions

Inactive Publication Date: 2008-10-08
NANCHANG HANGKONG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Although chemical and physical degradation can also be used to obtain oligomeric chitosan, these methods are difficult due to the control of reaction conditions and separation conditions, and the yield of chitosan with a degree of polymerization of 6-10 is low, and the molecular weight of the product obtained by degradation Inhomogeneous, wide distribution, high cost; due to the specificity of the enzymatic hydrolysis method, specific chains can be selectively cut off to produce specific oligosaccharides, and the reaction conditions are mild, and strong acids and bases are not used to avoid the formation of oligomers. destruction, will not cause environmental pollution, and is considered to be the most ideal method for chitosan degradation

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Example 1 Under the conditions of temperature 50°C, pH 5.0, and chitosan:enzyme ratio of 5:1, enzymolysis was carried out for 1 h, 4 h, 8 h, and 24 h respectively, the intrinsic viscosity was measured, and the molecular weight was calculated. The results showed that the average molecular weight of chitosan was degraded to 32949, 9770, 3831 and 1848 with the increase of the ratio of chitosan and enzyme and the prolongation of reaction time, and the degradation effect was obviously enhanced.

Embodiment 2

[0017] Example 2 With the steps of Example 1, enzymolysis 28hr, the filtrate after enzymolysis of chitosan was concentrated under reduced pressure until the final volume was 10% of the initial volume, then mixed with an equal volume of acetone to obtain a precipitate, at 4000 rpm The precipitate was collected by centrifugation for 30 minutes, eluted several times with 50% acetone, and then dried in a vacuum oven to obtain part A (average molecular weight about 1200). The remaining solution was concentrated to about 5% of the original volume under vacuum distillation, then 9 volumes of acetone were added to make it fully mixed to produce a precipitate, the precipitate formed was collected by centrifugation, washed with 90% acetone, and dried under reduced pressure to obtain part B (average Molecular weight is about 650).

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PUM

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Abstract

The invention relates to a method for preparing chitosan oligosaccharide by using trichoderma reesei cellulose. After purified chitosan oligosaccharide powder is swelled by using water, acetic acid solution is added to completely dissolve the chitosan oligosaccharide powder, aspergillus niger cellulose is dissolved by using ammonium acetate or sodium acetate aqueous solution, to prepare enzyme solution with a concentration of 0.5 to 2mg / ml; when the temperature is lower than 40 to 55DEG C, the enzyme solution is added to perform enzymolysis reaction, to obtain low molecular weight chitosan oligosaccharide, after performing acetone-water fractional precipitation, low molecular weight products with different distribution scopes can be obtained through concentration drying. The advantages of the invention is that the reaction conditions are mild, strong acids and strong alkalis are not adopted, the damage to the oligomer is avoided, the invention is free from environmental contamination, and is an ideal method for degrading the chitosan oligosaccharide.

Description

technical field [0001] The invention relates to a method for preparing chitosan oligosaccharide by using Trichoderma reesei cellulase. Background technique [0002] Oligosaccharides are also called oligosaccharides. In the past, disaccharides to ten sugars were called oligosaccharides, and now this range is generally expanded to twenty sugars, called oligosaccharides. Oligomeric chitin can be called chitooligosaccharide, and oligochitosan can be called chitooligosaccharide. For chitin and chitosan, chitosan with a relative molecular weight of less than 10,000 has many functions superior to high molecular weight chitosan, especially the five-nine sugars of chitin and chitosan with biological activity, In particular, the hexasaccharides and heptasaccharides have encouraging effects on tumor inhibition, and are the key areas of research and development at home and abroad. [0003] There are generally three methods for the preparation of oligomeric chitosan and oligomeric chit...

Claims

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Application Information

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IPC IPC(8): C12P19/04C12R1/885
Inventor 谢宇李明俊
Owner NANCHANG HANGKONG UNIVERSITY
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