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Preparation method of glycosylated and ordered porous membrane and uses thereof

A technology of glycosylation and porous membranes, applied in the field of ordered porous membranes, can solve problems such as unclustered effects, and achieve good application prospects, strong controllability, and mild conditions

Inactive Publication Date: 2008-10-15
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the recognition process of glycosyl and protein needs to realize the "cluster effect" to enhance the strength and specificity of glycosyl to protein recognition, so as to be used for high-sensitivity detection of proteins. Enrichment to achieve its cluster effect, there are deficiencies

Method used

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  • Preparation method of glycosylated and ordered porous membrane and uses thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] The glucose glycosylated styrene block copolymer with a molecular weight of 400,000 and a glycosyl mole percentage of 0.5% is dissolved in carbon disulfide to prepare a solution with a weight percentage concentration of 15%. Take 1 ml of the above solution and apply it to a clean glass surface, and form a film at 50% relative humidity at 5 degrees Celsius to obtain a film with an ordered porous structure. The porous membrane is observed with a scanning electron microscope, and the micropore diameter is 0.2±0.05 μm.

[0018] The above-mentioned glucose glycosylated ordered porous membrane (pore size 0.2 ± 0.05 micron) was immersed in 1 ml of peanut agglutinin / fluorescence-labeled concanavalin (PNA / FL-Con A) solution with a concentration of 0.1 g / L, Keep the temperature at 25 degrees Celsius for 10 minutes, take out the porous membrane, and measure the relative value of the fluorescence intensity as 108; immerse the above-mentioned glucose glycosylated ordered porous memb...

Embodiment 2

[0020] The mannose glycosylated styrene block copolymer with a molecular weight of 100,000 and a glycosyl mole percentage of 5% is dissolved in carbon disulfide to prepare a solution with a concentration of 5% by weight. Take 1 milliliter of the above solution and apply it to a clean glass surface, and form a film at 70% relative humidity and 25 degrees Celsius to obtain a film with an ordered porous structure. The porous membrane was observed with a scanning electron microscope, and the micropore diameter was 5.3±0.7 microns.

[0021] Immerse the above-mentioned mannose glycosylated ordered porous membrane (pore size 5.3±0.7 microns) in 1 ml of soybean lectin / fluorescence-labeled pea lectin (SBA / FL-PSA) solution with a concentration of 0.1 g / L, at 25 degrees Celsius Keep the temperature for 5 minutes, take out the porous membrane, and measure the relative value of the fluorescence intensity as 133; immerse the above-mentioned glucose glycosylated ordered porous membrane (pore...

Embodiment 3

[0023] The galactose glycosylated styrene block copolymer with a molecular weight of 10,000 and a glycosyl mole percentage of 30% is dissolved in carbon disulfide to prepare a solution with a concentration of 0.5% by weight. Take 1 milliliter of the above solution and apply it to a clean glass surface, and form a film at 90% relative humidity and 50 degrees Celsius to obtain a film with an ordered porous structure. The porous membrane was observed with a scanning electron microscope, and the micropore diameter was 19.8±2.3 microns.

[0024] The galactose glycosylated ordered porous membrane (pore size 19.8 ± 2.3 microns) was immersed in 1 ml of concanavalin / fluorescently labeled peanut aggregin (Con A / FL-PNA) solution with a concentration of 0.1 g / L , keep the temperature at 25 degrees Celsius for 10 minutes, take out the porous membrane, and measure the relative value of the fluorescence intensity to be 194; immerse the above-mentioned glucose glycosylated ordered porous memb...

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Abstract

The invention discloses a method for making a glycosylation sequential porous membrane and an application of the same. The method comprises the following steps that: (1) a saccharine block copolymer is dissolved into a solvent to obtain a polymer solution with a weight percentage of between 0.5 and 15 percent; (2) the polymer solution is casted to form a membrane at a relative humidity of between 50 and 90 percent and a temperature of between 5 and 50 DEG C to obtain the glycosylation sequential porous membrane with a aperture of between 0.2 and 20 micrometers. The glycosylation sequential porous membrane is used for the protein detection. The method adopts a hydrophilicity saccharine group to move toward the membrane surface under the induction action of water drops to obtain a sequential surface rich in glycosyl groups highly in a special area, uses the clustering effect of the glycosyl group to identify the protein into full play and evidently improves the detection sensitivity of the protein. The glycosylation sequential porous membrane made by the method has the advantages of simple preparation method, moderate technique condition and low production cost, etc.

Description

technical field [0001] The invention relates to an ordered porous membrane, in particular to a preparation method and application of a glycosylated ordered porous membrane. Background technique [0002] Ordered porous materials have broad application prospects in catalyst loading, gas sensing devices, optoelectronic devices, cell culture substrates, separation or adsorption media, etc. A series of methods for preparing ordered porous materials have been developed, such as lithography, etching, templating, self-assembly, and water-assisted methods. The water-assisted method, also known as the water vapor induction method or the water drop template method, uses condensed water as a template in a high-humidity environment to prepare an ordered porous film with uniform pore size distribution and tight arrangement on a solid substrate. Since Francois first used star-shaped polystyrene as a film material to obtain a honeycomb structure in a high-humidity environment (see G.Widaws...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J5/18C08J9/28C08L53/00G01N33/68
Inventor 徐志康柯蓓蓓万灵书孟祥麟章路遥
Owner ZHEJIANG UNIV