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Process for separating gibberellin A4 from the mixture of gibberellin A4 and A7

A separation method and gibberellin technology are applied in the fields of pest control, plant growth regulators, chemicals for biological control, etc. The effect of reasonable process route and easy operation

Inactive Publication Date: 2008-10-22
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above separation methods have defects such as poor control of separation speed, low separation purity, and expensive instruments, which should be improved.

Method used

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  • Process for separating gibberellin A4 from the mixture of gibberellin A4 and A7

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Add 60g GA to a 500ml beaker 4+7 Raw material, GA in raw material 4 with GA 7 The content of the content is 40% and 60% respectively, then add 10g tetramethylethylenediamine, add 120g ethanol in the beaker, make it dissolve and become transparent liquid after heating, place seven days at room temperature, have colorless, transparent crystal to separate out, Through NMR, UV, MS and other means, the crystal structure is obtained as GA 4 , the complexes of tetramethylethylenediamine and water molecules, while GA 7 then stay in the original solution, thus realizing the GA 4 with GA 7 The separation, the separation purity: 91.2%, the product yield: 92.7%.

Embodiment 2

[0025] Add 90g GA to a 500ml beaker 4+7 Raw material, GA in raw material 4 with GA 7 The content is respectively 40% and 60%, then add 17g tetramethylpropanediamine, add 180g acetone in the beaker, make it dissolve and be transparent liquid after heating, place seven days under room temperature, have colorless, transparent crystal to separate out, Through NMR, UV, MS and other means, the crystal structure is obtained as GA 4 , the complexes of tetramethylpropylenediamine and water molecules, while GA 7 then stay in the original solution, thus realizing the GA 4 with GA 7 The separation, the separation purity: 94.7%, the product yield: 96.5%.

Embodiment 3

[0027] Add 120g GA to a 500ml beaker 4+7 Raw material, GA in raw material 4 with GA 7 The contents of 40% and 60% respectively, then add 26g of tetramethyl butanediamine, add 120g of ethyl acetate in the beaker, make it dissolve into transparent liquid after heating, leave it at room temperature for seven days, there are colorless, transparent crystals Precipitation, through NMR, UV, MS and other means, the crystal structure is GA 4 , the complexes of tetramethylbutanediamine and water molecules, while GA 7 then stay in the original solution, thus realizing the GA 4 with GA 7 The separation, the separation purity: 93.5%, the product yield: 94.3%.

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Abstract

The invention discloses a method for separating gibberellin A4 from a mixture of gibberellin A4 and A7, the method is: the raw material of the mixture of gibberellin A4 and A7 is mixed with tetramethyl diamine according to a molar ratio of 1:1 to 2, an organic solvent is added, the mixture is heated until the raw material is completely dissolved, and then the solution is placed under minus 10 DEG C to 30 DEG C for enough long time until colorless and transparent crystals are precipitated; the obtained crystals are the complexes of gibberellin A4, tetramethyl diamine and water molecules. Compared with the prior art, the method for separating gibberellin A4 from a mixture of gibberellin A4 and A7 is reasonable in technological route and simple in operation, and the products obtained by the separation are high in yield and good in purity and have a social popularization value.

Description

(1) Technical field [0001] The present invention relates to a kind of gibberellin A 4 The separation method, especially one from gibberellin A 4 with A 7 gibberellin A 4 Methods. (2) Background technology [0002] Gibberellins (CAs) are important regulatory hormones in the process of plant growth and development, and have obvious regulatory effects on seed germination, stem and leaf growth, bolting, fruit set and tuber formation. It is widely used in a variety of food crops, and it is even more widely used in vegetables, which can obviously promote the yield and quality of crops and vegetables. [0003] Gibberellins are a class of diterpenoids with unique chemical structures, dense functional groups, and complex chemical properties. By the end of 2000, 127 gibberellins with different structures had been identified in plants and fungi. The difference between various gibberellins is only the number of functional groups and the difference in arrangement position, usually A...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D307/77C07D307/00A01P21/00
Inventor 金志敏马林林
Owner ZHEJIANG UNIV OF TECH
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