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Technique for extracting high-content effective composition from white peony root

An extraction process and technology for active ingredients, applied in the field of extracting traditional Chinese medicine ingredients, can solve the problems that aluminum oxide cannot be reused, is not suitable for pharmaceutical needs, and is not suitable for industrialized large-scale production.

Inactive Publication Date: 2008-11-05
NINGBO LIWAH PHARM CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The content of paeoniflorin in the Radix Paeoniae Alba extract extracted by this process can generally only reach 30% to 50%, which is relatively low and not suitable for pharmaceutical needs; Chinese Patent No. ZL00121532 discloses a method of using trioxide The total glucosides of paeony can be purified by di-aluminum column chromatography so that the content of paeoniflorin can reach 80%, but the di-aluminum oxide in the di-aluminum trioxide column chromatography cannot be reused, which is not suitable for industrialized large-scale production, and its cost is also extremely high. high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Add 300ML50% ethanol-water solution to 100G Radix Paeoniae Alba, heat and reflux for 1 hour, filter, concentrate, and dilute NaHCO 3 Adjust the pH of the solution to 8, use 30ML 20% ethanol-ethyl acetate, extract 3 times at a temperature of 5 degrees, combine the extracts, concentrate under reduced pressure, and dry in vacuo to obtain the crude extract of Radix Paeoniae Alba, put the crude extract of Radix Paeoniae Alba into Dissolve in ethanol, a hydrophilic solvent 10 times its weight, and use dilute alkali NaHCO 3 Adjust the pH value to 4, and use HPD-100 macroporous adsorption resin with polystyrene skeleton to adsorb the aqueous solution. After the adsorption reaches saturation, first wash away impurities with water until the Molish reaction is negative, and then use 20% to 90% of 5 times the amount of resin The flow rate (V / H) of the hydrophilic solvent of % final concentration 3 times of resin amount / hour elutes target substance, and eluent depressurizes back to ...

Embodiment 2

[0017] Add 200ML of 40% methanol-water solution to 100G of Radix Paeoniae Alba, heat and reflux for 2 hours, filter, concentrate, adjust the pH to 9 with NaOH, extract three times with 30ML of 40% methanol-ethyl acetate solution at 10°C, combine the extracts, and depressurize Concentrate, concentrate in vacuo, and dry in vacuo to obtain the crude extract of Radix Paeoniae Alba, put the crude extract of Radix Paeoniae Alba into 20 times its weight of hydrophilic solvent methanol to dissolve, adjust the pH value to 6 with dilute alkali NaOH, use polyphenylene The HPD-300 macroporous adsorption resin with ethylene skeleton absorbs the aqueous solution. After the adsorption reaches saturation, first wash off the impurities with water until the Molish reaction is negative, and then use a hydrophilic solvent with a final concentration of 20% to 90% of 10 times the amount of resin The target substance was eluted at a flow rate (V / H) of 5 times the amount of resin per hour, and the elu...

Embodiment 3

[0019] Add 150ML60% propanol-water solution to 100G white peony root, heat and reflux for 3 hours, filter, concentrate, and dilute KHCO 3 Adjust the pH of the solution to 6, extract three times with 30ML 60% propanol-ethyl acetate solution at 40°C, combine the extracts, concentrate under reduced pressure, concentrate in vacuo, and dry in vacuo to obtain the total crude extract of Radix Paeoniae Alba. The substance is dissolved in propanol, a hydrophilic solvent 40 times its weight, and is dissolved with dilute alkali KHCO 3 Adjust the pH value to 9, and use D101 macroporous adsorption resin with polyacrylic acid skeleton to absorb the aqueous solution. After the adsorption reaches saturation, first wash away the impurities with water until the Molish reaction is negative, and then use 20% to 90% of the final concentration of 20 times the amount of resin Hydrophilic solvent 10 times the flow rate (V / H) of the amount of resin / hour to elute the target substance, the eluent is dec...

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PUM

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Abstract

The invention provides an extraction technology of high-content white paeony root active ingredients, comprising crude extraction, dissolution, purification and such steps, the crude extraction adopts alcohol-water solution for extraction and uses alcohol ester solution for extraction, a white paeony root crude extract is obtained by concentration and drying, the purification adopts a macroporous absorption resin to carry out absorption and separation; the technology of the invention can greatly improve the content of paeoniflorin to 60 percent to 90 percent, the process has simple operation and lower cost, and the resin can be repeatedly used, which is applicable to industrial production.

Description

Technical field: [0001] The invention relates to a process for extracting Chinese medicine components from Chinese medicinal materials, in particular to an extraction process for extracting high-content active ingredients from the traditional Chinese medicine Radix Paeoniae Alba. Background technique: [0002] The traditional Chinese medicine Paeoniae Alba is the dried root of Paeoniae officinalis, a plant of the Ranunculaceae family. It has the effects of calming the liver and relieving pain, nourishing blood and regulating menstruation, astringing yin and antiperspirant; it is used for headache, dizziness, hypochondriac pain, abdominal pain, contraction pain in limbs, blood deficiency and chlorosis, irregular menstruation, spontaneous Sweat, night sweat. Pharmacological studies have shown that Paeoniae Alba has antispasmodic, analgesic, immune regulation, and anti-inflammatory effects. The main active ingredients contained in Radix Paeoniae Alba are paeoniflorin, paeonifl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/71A61P29/00A61P15/00A61P7/06A61P1/16A61K125/00
Inventor 周海滨江海龙周戎陈旦凤
Owner NINGBO LIWAH PHARM CO LTD