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Method for extracting lycopene from tomato pomace

A tomato pomace, lycopene technology, applied in the direction of bulk chemical production, fermentation, etc., can solve the problem of unrestricted pectinase and cellulase activity, yield and purity, unsatisfactory operability, and restrictions on tomato The widespread use of erythromycin and other issues, to achieve the effect of improving utilization and added value, important social significance, and reducing production costs

Inactive Publication Date: 2008-11-05
上海博翼有机农业技术发展有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The current production and extraction process of lycopene in China is mainly extracted from tomato paste with organic solvents, or directly with CO 2 Supercritical extraction, due to the low extraction efficiency of these methods, the high production cost limits the widespread use of lycopene
Patent No. 200610173351.8 involves the use of biological enzymes to extract lycopene from tomato pomace. The pectinase and cellulase used in this method are enzymatically hydrolyzed under acidic conditions, but the method does not limit pectinase and cellulase. The activity of cellulase, and the active biological enzymes should participate in the enzymatic hydrolysis reaction, the content of active substances in commercially available products is also very different, and the above two biological enzymes cannot completely remove lycopene from Released from the tomato peel, so the yield, purity and operability of the method are not ideal

Method used

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  • Method for extracting lycopene from tomato pomace

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Compound biological enzyme ratio (neutral protease, neutral cellulase and alkaline pectinase by weight ratio is 0.9:1:1.1)

[0024] Pour 10Kg of tomato peel residue into a 50L reaction container, add 20Kg of deionized water, and adjust the pH to 7.5; stir and heat to 50°C, add 5g of compound biological enzyme to react for 6 hours; then inactivate the enzyme at 70°C for 12 minutes; Centrifuge dehydration to obtain the enrichment, elute with alcohol, cool to 4°C to concentrate and crystallize, and obtain 0.82Kg of crude lycopene with a content of 11%; 2 Supercritical extraction and separation yielded 82 g of 95% lycopene crystals.

Embodiment 2

[0026] Compound biological enzyme ratio (neutral protease, neutral cellulase and alkaline pectinase in a weight ratio of 1:1:1)

[0027] Pour 20Kg of tomato skin residue into a 100L reaction container, add 60Kg of deionized water, and adjust the pH to 8.5; stir and heat to 55°C, add 20g of compound biological enzyme to react for 5 hours; then inactivate the enzyme at 72°C for 12 minutes; Centrifuge dehydration to obtain the enriched product, elute with alcohol, cool to 4°C to concentrate and crystallize, and obtain 1.72Kg of crude lycopene with a content of 10.5%; 2 Supercritical extraction and separation obtained 160 g of 95.5% lycopene crystals.

Embodiment 3

[0029] Compound biological enzyme ratio (neutral protease, neutral cellulase and alkaline pectinase by weight ratio is 1:1.1:0.9)

[0030] Pour 10Kg of tomato skin residue into a 100L reaction container, add 50Kg of deionized water, and adjust the pH to 9.0; stir and heat to 60°C, add 15g of compound biological enzyme to react for 4 hours; then inactivate the enzyme at 75°C for 10 minutes; Centrifuge dehydration to obtain the enrichment, elute with alcohol, cool to 4°C to concentrate and crystallize, and obtain 0.87Kg of crude lycopene with a content of 10%; 2 Supercritical extraction and separation yielded 85 g of 95% lycopene crystals.

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Abstract

The invention relates to a method for extracting lycopin with tomato skins and marcs and belongs to the field of the extraction of native compounds. By utilizing the composition of three kinds of neutral and alkalic bio-enzymes under neutral and alkalinity leaning condition, the method realizes complete enzymolysis of the tomato skins and marcs and complete dissociation of the lycopin, and by combining with the extraction technique that is combined with CO2 supercritical technique, the method for extracting the lycopin leads wastes to be reclaimed and recycled, greatly improves the utilization rate and the added value of agricultural resources, and simultaneously reduces environmental pollution; the method for extracting the lycopin from the tomato skins and marcs achieves that the lycopin extraction rate is larger than 80 percent and the purity of the obtained product is larger than 95 percent, does not have solvent residue, and holds a lead in the world concerning the research. A bio-enzymatic method of the method for extracting the lycopin from the tomato skins and marcs adopts the three kinds of enzymes that have similar enzymolysis conditions, implements the enzymolysis under the same condition, and does not need to adjust the pH value of an enzymolysis solution for multiple times, thus having simple technique and easy operation, the production cost of the lycopin can be greatly reduced by adopting the production technique of the invention, and the method for extracting the lycopin from the tomato skins and marcs has remarkable economical benefits and important social meanings.

Description

technical field [0001] The invention relates to the field of extraction of natural compounds, in particular to a method for extracting lycopene by using tomato pomace. Background technique [0002] With the acceleration of the construction of a new socialist countryside, new sources of pollution continue to emerge in the process of rural urbanization. Among them, the large-scale fruit and vegetable wholesale markets and fruit juice processing industries in my country produce 3 million tons of tomato peels annually. These wastes contain certain nutrients. The components, if not treated, will be piled up for a long time, exposed to the sun, rain, air and microorganisms, and will quickly deteriorate, produce unpleasant stench, breed mosquitoes and other insect pests, seriously affect the surrounding environment of cities and towns, and affect people's life and health. healthy. The comprehensive utilization rate of these wastes is poor, and some valuable functional components suc...

Claims

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Application Information

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IPC IPC(8): C12P5/02
CPCY02P20/54
Inventor 汪红兵吴瑞兵陈红霞
Owner 上海博翼有机农业技术发展有限公司
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