Compound formulation containing lactobacillus leavening and preparation and use thereof

A technology of lactic acid bacteria fermentation and compound preparation, which is applied in the field of biological preparations, can solve problems such as reducing the body's own immunity and harming healthy cells, and achieve the effects of improving human immunity, improving immunity, and strengthening immune function

Inactive Publication Date: 2011-06-15
福建杨振华851生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chemotherapy and radiotherapy are currently the most commonly used methods for treating cancer and tumors. These not only kill cancer cells, but also do great harm to healthy cells of the human body, and seriously reduce the body's own immunity and cause complications.

Method used

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  • Compound formulation containing lactobacillus leavening and preparation and use thereof

Examples

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Effect test

preparation example Construction

[0012] The preparation method of the live lactic acid bacteria fermentation product is as follows: inoculate the lactic acid bacteria strains into the lactic acid bacteria fermentation medium at 36-42°C, ferment at 37-42°C for 5-52 hours, concentrate, freeze-dry or spray-dry to obtain the live lactic acid bacteria fermentation product .

[0013] The preparation method of the non-live lactic acid bacteria fermentation product is as follows: inoculate the lactic acid bacteria strains into the lactic acid bacteria fermentation medium at 36-42°C, ferment at 37-42°C for 5-52 hours, concentrate, sterilize at 100-115°C, and spray dry to obtain Non-live lactic acid bacteria ferment.

[0014] Mix the lactic acid bacteria fermented product and soybean saponin according to the mass ratio, pass through a 40-100 mesh sieve, add (total mass of the above mixture) 0.5% magnesium stearate, mix, pass through a 40-100 mesh sieve, fill the capsule, Prepared into a compound preparation.

[0015]...

Embodiment 1

[0022] Culture of lactic acid bacteria fermentation

[0023] Lactobacillus acidophilus was inserted into the slant medium, the culture temperature was 37°C, and the culture time was 48 hours. Transfer the slant strains into a 500mL Erlenmeyer flask filled with 100mL medium, and culture at 40°C for 24 hours. Then the strain was inserted into a 500mL Erlenmeyer flask containing 100mL of culture medium and left to expand at 40°C for 24 hours, and 2000mL of the strain after the expansion culture was merged into a 5L suction filter bottle, and the strain in the suction filter flask was inoculated. In the GY345-SM three-stage liquid strain fermentation tank (Jiangsu Institute of Technology Bioengineering Equipment Joint Venture Factory) equipped with 40L, 40 ℃ static culture for 24 hours. Transfer the fermentation liquid in the strain fermenter into a 1000L fermenter with 400L fermentation medium, and culture it statically at 37°C for 48-56 hours. When the lactic acid of the fermen...

Embodiment 2

[0027] With Lactobacillus acidophilus, Streptococcus thermophilus, Lactobacillus reuteri separate slant culture, cultured strains are respectively inserted into Erlenmeyer flask liquid medium culture, the inoculum size is 3% (bacteria culture starter capacity (L) / Substratum capacity (L), three kinds of bacterial classifications after the cultivation are merged by the ratio of 1: 1: 1 and then expanded and cultivated in the Erlenmeyer flask liquid medium, then carry out expanded fermentation production as in implementing 1, the final fermented liquid Lactic acid is greater than 5.5mg / g, and acidity is greater than 10mg / g. The culture medium and culture temperature and time of this implementation are the same as implementation 1.

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Abstract

The invention provides a compound preparation containing lactobacillus leavening and a method for preparing and an application of the same. The compound preparation is an active bacteria preparation or an inactive bacteria preparation which is made by freeze drying or spray drying. The ingredients by mass percentage of the compound preparation comprise 10 to 98.5 percent of lactobacillus leavening and 1.5 to 90 percent of soyasaponins. The preparation method is as follows: the lactobacillus leavening and the soyasaponins are mixed according to mass mixture ratio, and are subject to sieving, granulation to obtain the compound preparation. The compound preparation has a function of improving immunity, antitumor effect and the effectiveness of antitumor drugs or can be used directly as an antitumor drug or health care product. The compound preparation of the invention is active bacteria preparation or an inactive bacteria preparation and has advantages of high biological activity, effective improvement on immunity of human bodies, anticancer or cancer-preventing effect, simple preparation method, strong operability, suitability for mass production as well as desirable economic and social benefits.

Description

technical field [0001] The invention belongs to the field of biological preparations, and more specifically relates to a compound preparation containing fermented lactic acid bacteria and its preparation method and application. Background technique [0002] Tumor is the formation of monoclonal abnormal hyperplasia caused by cells in local tissues losing control of their growth at the gene level under the action of various tumorigenic factors. It is generally divided into two categories: benign tumors and malignant tumors. Tumor is currently the most serious type of disease that endangers human health, which is what we usually call "cancer". Chemotherapy and radiotherapy are currently the most commonly used methods for treating cancer and tumors. These not only kill cancer cells, but also do great harm to healthy cells of the human body, and seriously reduce the body's own immunity and cause complications. [0003] Lactic acid bacteria are a kind of microorganisms that are v...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K35/74A61K9/14A61K9/16A61K9/20A61K9/48A61P37/04A61P35/00A23L1/29C12P1/04A61K36/48A23L33/00A61K35/744
Inventor 陈旭东张厚熙吴斌秀廖庆禄李勤陈彩萍
Owner 福建杨振华851生物科技股份有限公司
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