Uses of BMP-7 in preparing medicament for preventing and/or treating liver fibrosis

A technology of liver fibrosis and BMP-7, which is applied in the direction of drug combination, drug delivery, non-active ingredients of polymer compounds, etc., to achieve the effect of good application prospects

Inactive Publication Date: 2008-11-12
TONGJI UNIV
View PDF0 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there have been no reports at home and abroad on the research on the effect of BMP-7 on experimental liver fibrosis

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Uses of BMP-7 in preparing medicament for preventing and/or treating liver fibrosis
  • Uses of BMP-7 in preparing medicament for preventing and/or treating liver fibrosis
  • Uses of BMP-7 in preparing medicament for preventing and/or treating liver fibrosis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0031] 1. Successfully prepared a rat liver fibrosis model:

[0032] Repeated intraperitoneal injection of pig serum was used to induce an immune rat liver fibrosis model. Porcine serum 0.5ml / time, intraperitoneal injection of experimental rats, twice a week, a total of 8 weeks. Such asFigure 1 Show.

[0033] 2. Successfully isolate and culture hepatocytes and hepatic stellate cells from model animals.

[0034] Two enzymes (type I collagenase and pronase E) portal vein perfusion and shaved centrifugation were used to separate the hepatocytes and hepatic stellate cells of experimental animals. Such as figure 2 Shown.

[0035] 3. Observe the effects of BMP-7 on the production and secretion of collagen in primary hepatocytes.

[0036] The BMP-7 in the present invention was purchased from Sigma Company in the United States, with a purity of greater than 95% (each packaged BMP-7 freeze-dried powder containing 0.2μm BMP-7, 33% cyanomethane, 0.1% trifluoroacetic acid, 0.5mg cattle Seru...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to the application of BMP-7 in preparing drugs for preventing and / or treating hepatic fibrosis and belongs to the medical treatment technical field. The preparation comprises the steps of: preparing a hepatic fibrosis model of rats through an animal model experiment and a vitro cell experiment, separating liver cells and hepatic stellate cells of an animal to be tested, observing the affect of bone morphogenetic protein BMP-7 on experimental hepatic fibrosis. The results show that BMP-7 can restrain the activity of a signaling pathway protein Smad 1 / 3 of TGF-beta1 in cells and the expression of TGF-beta1.The results of the animal experiment show that BMP-7 can remarkably prevent and treat experimental hepatic fibrosis.

Description

Technical field [0001] The invention belongs to the field of preparing medicines for the prevention and treatment of chronic liver disease, liver fibrosis and liver cirrhosis. Background technique [0002] Liver fibrosis is the common pathological basis and pathological feature of the development of various chronic liver diseases. Progressive liver fibrosis can cause liver function decline, and then liver cirrhosis characterized by the regeneration of liver lobules, which eventually leads to liver failure. In recent years, people have conducted in-depth and extensive discussions on the mechanism of liver fibrosis, gradually changing the traditional view that liver fibrosis is irreversible [1] . However, in actual clinical work, people still lack effective measures for the treatment of liver fibrosis. my country is a country with a high incidence of liver disease. Every year, chronic liver disease fibrosis and even the development of liver cirrhosis bring huge losses to many patie...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/18A61K47/42A61K9/08A61P1/16
Inventor 杨长青常义忠杨丽王胜兰
Owner TONGJI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap