Preparing polypyrrole package prussian blue nano particle for electrochemical analysis by microemulsion method

A Prussian blue and nanoparticle technology is applied in the field of preparing polypyrrole-coated Prussian blue nanoparticles by microemulsion method, which can solve the problems of easy agglomeration and poor stability of nanoparticles.

Inactive Publication Date: 2008-12-03
ZHEJIANG NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0018] The purpose of the present invention is to overcome the shortcomings of poor stability of Prussian blue and the easy aggregation of its nanoparticles, to provide a preparation method of Prussian blue nanoparticles wrapped in polypyrrole and its application in the field of electroanalysis and biosensing

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Preparation of polypyrrole-coated Prussian blue nanoparticles: prepare 0.5M dioctyl sodium sulfosuccinate in 2ml of isooctane, add 0.09ml of water to control the final water / surfactant molar ratio of 12.22, stir until clear; drop Add 0.05ml of an aqueous solution containing 1M KCl and 0.1M HCl, stir until clear; add 0.04ml of 0.1M FeCl dropwise 3 , stirred until clear; dropwise added 100μl pyrrole monomer and stirred for two hours; added dropwise 0.04ml 0.1M K 4 Fe(CN) 6 Stir overnight; wash with ethanol and water repeatedly by centrifugation to remove impurities, and finally disperse with water to obtain a colloidal solution of Prussian blue nanoparticles wrapped by polypyrrole.

Embodiment 2

[0029] Preparation of polypyrrole-coated Prussian blue nanoparticles modified gold electrode and its application in H 2 o 2 Electrochemical detection: firstly, a gold disk electrode with a diameter of 5 mm was immersed in a 2 mM cysteine ​​aqueous solution to assemble for 24 hours, rinsed with water and dried; the colloid solution of polypyrrole-coated Prussian blue nanoparticles in Example 1 was added dropwise to the electrode surface, rinse with water after 24 hours.

[0030] The polypyrrole-coated Prussian blue nanoparticle-modified gold electrode, the Ag / AgCl reference electrode, and the platinum wire counter electrode were placed in a pH 6 phosphate buffer, and the modification was measured by cyclic voltammetry or potentiostatic method on an electrochemical workstation. Electrode pair H 2 o 2 electrochemical response.

Embodiment 3

[0032] The polypyrrole-coated Prussian blue nanoparticle-modified gold electrode is used for the preparation of a glucose biosensor and for the detection of glucose: 1 μl of 10 mg / ml glucose oxidase is dropped on the surface of the polypyrrole-coated Prussian blue nanoparticle-modified gold electrode described in Example 2 Mix with 1 μl of 1% glutaraldehyde aqueous solution, rinse with water after drying.

[0033] Put the prepared glucose biosensor together with the Ag / AgCl reference electrode and the platinum wire counter electrode into the phosphate buffer solution with pH 6, and measure the effect of the modified electrode on the glucose by cyclic voltammetry or potentiostatic method on the electrochemical workstation. electrochemical response.

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Abstract

The invention relates to the preparation and the application of a nanometer material, in particular to a Prussian blue nanometer particle coated with polypyrrole, which is prepared by the micro-emulsion method and is used for electrochemical analysis as a modified electrode. The invention aims to overcome the defects that the stability of the Prussian blue is poor and the nanometer particles thereof are easy to conglomerate. The preparation and the application comprise the following steps: the Prussian blue nanometer particle coated with polypyrrole is prepared by the micro-emulsion method; the Prussian blue nanometer particle coated with polypyrrole is fixed by cysteine modifying a noble metal electrode surface; the prepared modified electrode of the Prussian blue nanometer particle coated with polypyrrole can be used for the detection of H2O2 with good stability and high sensitivity; and an enzyme based biosensor can be prepared by further fixing redoxase on the surface of the modified electrode of the Prussian blue nanometer particle coated with polypyrrole, thereby realizing the detection and the analysis of enzyme-related zymolyte.

Description

(1) Technical field [0001] The invention relates to the preparation and application of nanometer materials, in particular to the preparation of polypyrrole-wrapped Prussian blue nanoparticles by a microemulsion method, and the modified electrode is used for electrochemical analysis. (2) Background technology [0002] h 2 o 2 Analytical testing is very important. Food, clothing, paper, printing and dyeing industries use a lot of H 2 o 2 , remain in the product or be discharged into the environment and affect human health. In addition, the peroxide content in the body is related to many diseases in the human body. The study found that the reduced form of Prussian blue, Prussian white, can catalyze O 2 with H 2 o 2 electrochemical reduction, and the oxidized form of Prussian blue can catalyze H 2 o 2 Oxidation. Research suggests that the three-dimensional structure of Prussian blue can accommodate small molecules of O 2 with H 2 o 2 , and is catalyzed by four diva...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327G01N27/30
Inventor 缪煜清陈建荣邱静霞刘继伟
Owner ZHEJIANG NORMAL UNIVERSITY
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