Preparing polypyrrole package prussian blue nano particle for electrochemical analysis by microemulsion method

Abstract

The invention relates to the preparation and the application of a nanometer material, in particular to a Prussian blue nanometer particle coated with polypyrrole, which is prepared by the micro-emulsion method and is used for electrochemical analysis as a modified electrode. The invention aims to overcome the defects that the stability of the Prussian blue is poor and the nanometer particles thereof are easy to conglomerate. The preparation and the application comprise the following steps: the Prussian blue nanometer particle coated with polypyrrole is prepared by the micro-emulsion method; the Prussian blue nanometer particle coated with polypyrrole is fixed by cysteine modifying a noble metal electrode surface; the prepared modified electrode of the Prussian blue nanometer particle coated with polypyrrole can be used for the detection of H2O2 with good stability and high sensitivity; and an enzyme based biosensor can be prepared by further fixing redoxase on the surface of the modified electrode of the Prussian blue nanometer particle coated with polypyrrole, thereby realizing the detection and the analysis of enzyme-related zymolyte.

Description

(1) Technical field

[0001] The invention relates to the preparation and application of nanometer materials, in particular to the preparation of polypyrrole-wrapped Prussian blue nanoparticles by a microemulsion method, and the modified electrode is used for electrochemical analysis. (2) Background technology

[0002] h 2 o 2 Analytical testing is very important. Food, clothing, paper, printing and dyeing industries use a lot of H 2 o 2 , remain in the product or be discharged into the environment and affect human health. In addition, the peroxide content in the body is related to many diseases in the human body. The study found that the reduced form of Prussian blue, Prussian white, can catalyze O 2 with H 2 o 2 electrochemical reduction, and the oxidized form of Prussian blue can catalyze H 2 o 2 Oxidation. Research suggests that the three-dimensional structure of Prussian blue can accommodate small molecules of O 2 with H 2 o 2 , and is catalyzed by four diva...