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Method for measuring biological degradation rate of water-soluble superpolymer

A technology of water-soluble polymers and determination methods, which is applied in the determination/inspection of microorganisms, biochemical equipment and methods, and measuring devices, etc., which can solve problems such as imprecise methods, low accuracy, and large differences, and achieve determination The effect of scientific method, improving accuracy and reducing experimental error

Inactive Publication Date: 2008-12-03
TIANJIN UNIVERSITY OF TECHNOLOGY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

For the biodegradation of water-soluble polymers, since the above-mentioned methods are all determined by indirect methods, the methods are not rigorous, so the accuracy is low and the error is large, especially the conclusions drawn by testing the same sample with different methods are sometimes different. very big

Method used

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  • Method for measuring biological degradation rate of water-soluble superpolymer
  • Method for measuring biological degradation rate of water-soluble superpolymer
  • Method for measuring biological degradation rate of water-soluble superpolymer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Example 1: Determination of the biodegradation rate of polyaspartic acid, the molecular weight of the polyaspartic acid sample is about 2300.

[0016] (1) Three-group bottle liquid culture method and detection of total organic carbon:

[0017] In the three-group bottle liquid culture method, the minimum purity of the water used is double-distilled water and the soluble organic carbon is less than 1mg / L; the minimum purity of the reagents used is analytical pure; There is no carbon-containing compound in the wall; the microbial strains come from the fresh activated sludge filtrate obtained from the biochemical tank of a sewage treatment plant and filtered to remove impurities. The dominant bacteria are Zoogloea, Comamonas Genus (Comamonas), Pseudomonas (Pseudomonas) and Micrococcus (Micrococcus), etc., the concentration of live bacteria in the inoculum solution is 1.0×10 4 ~1.0×10 5 CFU / mL; Inorganic salt basal medium solution is an aqueous solution composed of the fol...

Embodiment 2

[0028]Example 2: Determination of the biodegradation rate of polyacrylic acid, the molecular weight of the polyacrylic acid sample is about 2100. In addition to adding 100.0 mg of polyacrylic acid (equivalent to 4.76×10 -5 mol), the amount converted into total organic carbon is 50 mg, other such as three groups of bottles of liquid culture method and detection of total organic carbon, the method and step of determining the degradation rate through calculation are exactly the same as in Example 1, and the detection results of the three groups of bottles And the degradation rate determined by calculation is shown in the following table:

[0029]

[0030] Note: The data of the three groups of bottles in the table is the content of total organic carbon in each bottle after degradation, in mg / 1000mL.

[0031] The table shows: the microbial degradation ability to polyacrylic acid is up to 21.53%, the maximum error of parallel determination is 1.84%, the test data has good repeat...

Embodiment 3

[0032] Embodiment 3: the mensuration of the biodegradation rate of polyaspartic acid chitosan copolymer, polyaspartic acid and deacetylated chitosan copolymerization generation polymerization degree is the polyaspartic acid chitosan of 17%~19% A sugar copolymer with a molecular weight of about 2500. In addition to adding 108.7mg of copolymer (equivalent to 4.34×10 -5 mol), the amount converted into total organic carbon is 50 mg, other such as three groups of bottles of liquid culture method and detection of total organic carbon, the method and step of determining the degradation rate through calculation are exactly the same as in Example 1, and the detection results of the three groups of bottles And the degradation rate determined by calculation is shown in the following table:

[0033]

[0034] Note: The data of the three groups of bottles in the table is the content of total organic carbon in each bottle after degradation, in mg / 1000mL.

[0035] The table shows: the mi...

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Abstract

A detection method for the biodegradation rate of a water soluble superpolymer adopts a liquid culture method with three groups of bottles: culture bottles, neutral bottles and toxic bottles; liquids in the culture bottles and the toxic bottles are cultured for 28 days under specific conditions; the total organic carbon content of each sample liquid is respectively detected and calculated; and the biodegradation rate thereof is determined according to the change value of the total organic carbon before and after the culture. The method has the advantages as follows: the method is scientific, reasonable, simple and practical; a method that the organic substances in the liquids are directly detected is adopted, thereby improving the precision of the detection results; before the contents of the total organic carbon and the inorganic carbon in each treating liquid are detected, a ultrasonic cell disruption method is carried out on the basis of the complete biodegradation theory, the processing method can keep the intermediate metabolites transformed by the incomplete biodegradation in the treating liquids, the detected biodegradation rate only relates to a part of superpolymers which are completely transformed into CO2 and H2O, so that the detection method is more scientific and stricter.

Description

(1) Technical field [0001] The invention relates to the technical field of using microorganisms to degrade water-soluble high polymers, in particular to a method for measuring the biodegradation rate of water-soluble high polymers. (2) Background technology [0002] In recent years, the degradation of polymers has been a hot research topic at home and abroad. Microbes have been applied to the biodegradation of water-soluble polymers. Through the absorption and utilization of water-soluble polymers by microorganisms, the polymers are environmentally friendly. hazards are reduced to a minimum. At present, there are many methods for the determination and evaluation of the biodegradability of water-soluble polymers by microorganisms, including: detection of CO produced during the degradation process 2 , using it as a measurement index to evaluate the biodegradability, such as the STURM method; taking BOD / COD as a measurement index to test the O in the biodegradation process 2 ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/00C12Q1/02
Inventor 吴新世岳红雨孙波王菁师慈刁虎欣
Owner TIANJIN UNIVERSITY OF TECHNOLOGY
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