Manganese ion diagnosis/determination reagent kit and method for determining manganese ion concentration

A manganese ion, reagent technology, applied in the field of medical/food/environmental inspection and determination

Inactive Publication Date: 2008-12-17
SUZHOU ANJ BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Neutron excitation analysis (NAA) is the best method, but can only be determined in some well-conditioned laboratories

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] The manganese ion diagnosis / measurement reagent of the present embodiment is a single reagent, comprising:

[0042] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0043] Stabilizer 500mmol / L

[0044] Reduced coenzyme 0.25mmol / L

[0045] Ammonia ion 2mmol / L

[0046] Oxaloacetic acid 20mmol / L

[0047] Oxaloacetate decarboxylase 10000U / L

[0048] Alanine dehydrogenase 10000U / L

[0049] Glycine oxidase 10000U / L

[0050] After the reagents are all dissolved and prepared, they are divided into bottles and freeze-dried to make dry powder reagents; before use, add purified water and reconstitute for use.

[0051] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance 1.8±0.7, test main wavelength 340nm, test subwavelength 405nm, volume ratio of manganese ion sample to reagent is 1 / 25 , the reaction direction is negative reaction (decreasing reaction), the delay time is about 2 minutes, and the detec...

Embodiment 2

[0054] The manganese ion diagnosis / measurement reagent of the present embodiment is a double reagent, comprising:

[0055] Reagent 1

[0056] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0057] Stabilizer 50mmol / L

[0058] Reduced coenzyme 0.25mmol / L

[0059] Ammonia ion 2mmol / L

[0060] Oxaloacetic acid 20mmol / L

[0061] Reagent 2

[0062] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0063]Stabilizer 500mmol / L

[0064] Oxaloacetate decarboxylase 10000U / L

[0065] Alanine dehydrogenase 10000U / L

[0066] Glycine oxidase 10000U / L

[0067] After the reagents are all dissolved and prepared, they are divided into bottles to make liquid double reagents, which can be used directly.

[0068] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance 1.8±0.7, test main wavelength 340nm, test subwavelength 405nm, volume ratio of manganese ion sample to reagent 1 and reagent 2 It is ...

Embodiment 3

[0071] The manganese ion diagnosis / measurement reagent of the present embodiment is three reagents, comprises:

[0072] Reagent 1

[0073] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0074] Stabilizer 50mmol / L

[0075] Reduced coenzyme 0.25mmol / L

[0076] Ammonia ion 2mmol / L

[0077] Oxaloacetic acid 20mmol / L

[0078] Reagent 2

[0079] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0080] Stabilizer 500mmol / L

[0081] Alanine dehydrogenase 10000U / L

[0082] Glycine oxidase 10000U / L

[0083] Reagent 3

[0084] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0085] Stabilizer 500mmol / L

[0086] Oxaloacetate decarboxylase 10000U / L

[0087] After all the reagents are dissolved and prepared, they are divided into bottles to make liquid three reagents, which can be used directly.

[0088] When measuring manganese ion concentration, set on the automatic biochemical analyzer: temperature 37°C, reaction t...

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PUM

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Abstract

The invention relates to a kit for diagnosing/mensurating manganese ions by utilizing the technologies of the enzymatic cycling amplification method, the enzymic colorimetric method and the enzyme linked immunosorbent assay. The invention further relates to a method, a principle and the composition and the components of a reagent for mensurating the concentration of the manganese ions, and belongs to the technology field of medical/food/environmental inspection and measurement. The main components of the kit include a buffer solution, reduced coenzyme, oxaloacetic acid, ammonia ions, oxaloacetic decarboxylase, alanine dehydrogenase, glycine oxidase and a stabilizer. Through mixing a sample and the reagent by a certain volume ratio, a series of enzymatic reactions occur, then the reactant is placed under an ultraviolet/visible light analyzer, and the velocity of the decrease in absorbance at 340nm of the dominant wavelength is detected, thereby mensurating the concentration of the manganese ions.

Description

technical field [0001] The invention relates to a manganese ion diagnosis / measurement kit, and at the same time, the invention also relates to a method for measuring manganese ion concentration, which belongs to the technical field of medicine / food / environmental inspection and determination. Background technique [0002] As a trace element, manganese is an indispensable and necessary component in the human body, and it is also a substance that is toxic to the human body. In the natural state, it exists in divalent, trivalent and tetravalent forms, and the lower the degree of oxidation, the higher the toxicity. There are generally eight different oxidation states of manganese. The biological significance of manganese is its diverse functions as a cofactor in the human body. However, in many enzyme activation reactions, Mn 2+ and Mg 2+ Can replace each other. [0003] Commonly used graphite furnace atomic absorption spectrophotometry, atomic emission ICP-OES. Neutron exc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/84G01N21/31C12Q1/26C12Q1/00
Inventor 王尔中
Owner SUZHOU ANJ BIOTECHNOLOGY CO LTD
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