Decolored and debitterized Momordica grosvenori extract and preparation method thereof
A Luo Han Guo extract, debittering technology, applied in the direction of drug combination, medical formula, plant raw materials, etc., can solve the problems of resin decolorization, deodorization effect is not ideal, unfavorable decolorization, deodorization treatment, deep color of the extract, etc., to achieve Better product quality, avoiding low membrane filtration yield, and increasing content
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Embodiment 1
[0015] Embodiment 1 of the present invention: the preparation method of decolouring, debittering Luo Han Guo extract, its steps are as follows:
[0016] 1) Wash the fresh Luo Han Guo fruit, and kill it in hot water at 90°C for 1 minute;
[0017] 2) crush the dried Luo Han Guo and squeeze the juice, put the residue in 4 times the weight of pure water and soak it at room temperature for 4 times, and squeeze to obtain the squeeze liquid;
[0018] 3) Combine the fruit juice and squeezed liquid, centrifuge to remove impurities, and put the supernatant into ADS-21 macroporous adsorption resin;
[0019] 4) Wash the adsorption resin with water until the effluent is colorless, then elute with a 1% (weight percentage concentration) sodium carbonate solution 6 times the weight of the resin to remove pigment and impurities, and then wash with pure water until the effluent is neutral;
[0020] 5) elute the adsorption resin with 4 times the weight of the resin in 65% ethanol to remove the ...
Embodiment 2
[0023] Embodiment 2 of the present invention: prepare decolorization, debitter Luo Han Guo extract according to the following steps:
[0024] 1) Wash the fresh Luo Han Guo fruit, and kill it in hot water at 85°C for 1 minute;
[0025] 2) crush the dried Luo Han Guo and squeeze the juice, soak the residue in 5 times the weight of pure water at room temperature for 2 times, and squeeze to obtain the squeeze liquid;
[0026] 3) Combine the fruit juice and squeezed liquid, centrifuge to remove impurities, and use ADS-7 type macroporous adsorption resin to absorb the centrifuged supernatant;
[0027] 4) Wash the adsorption resin with water until the effluent is colorless, then elute with 2% (weight percentage concentration) sodium bicarbonate solution 10 times the weight of the resin to remove pigment and impurities, and then wash with pure water until the effluent is neutral;
[0028] 5) Elute the adsorption resin for removing pigment and impurities with 35% ethanol solution 6 ti...
Embodiment 3
[0031] Embodiment 3 of the present invention: prepare decolouring, debittering Luo Han Guo extract according to the following steps:
[0032] 1) Wash the fresh Luo Han Guo fruit and kill it in 80°C hot water for 2 minutes;
[0033] 2) crush and squeeze the dried Momordica grosvenori, soak the residue in 3 times the weight of water at room temperature for 5 times, and squeeze to obtain squeezed liquid;
[0034] 3) Combine the fruit juice and squeezed liquid, centrifuge to remove impurities, and put the supernatant into ADS-8 macroporous adsorption resin;
[0035] 4) Wash the adsorption resin with water until the effluent is colorless, then elute with 0.2% (weight percent concentration) sodium hydroxide solution twice the weight of the resin to remove pigment and impurities, and then wash with water until the effluent is neutral;
[0036] 5) eluting the adsorption resin for removing pigment and impurities with 50% ethanol solution twice the weight of the resin, and collecting t...
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