Matted bamboo tip tissue culture medium and tissue culture and rapid propagation method

A tissue culture medium, tissue culture rapid propagation technology, applied in horticultural methods, botanical equipment and methods, cultivation and other directions, can solve the problems of difficult tissue culture, difficult to regenerate plants, etc., achieve low cost, save mother bamboo, The effect of high reproduction rate

Inactive Publication Date: 2009-01-07
ZHEJIANG FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Under normal circumstances, embryo-derived callus is easy to obtain regenerated plants, and it is also easier to induce callus or regenerated plants from young tissues or derived parts of regenerated plants. It is difficult to culture explants from mature bamboos. It is difficult to induce regenerated plants. After searching, there has been no report on the method of using the buds of mature bamboo as explants to prepare effective tissue culture medium for rapid propagation of bamboo

Method used

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  • Matted bamboo tip tissue culture medium and tissue culture and rapid propagation method

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Experimental program
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Effect test

Embodiment 1

[0018] Embodiment 1 (composition and proportioning thereof of embodiment 1 in the control table 1, embodiment 1-7 is the effective tissue culture medium that preparation proliferates clustered shoot and rooting usefulness): add 2.0mg with general-purpose MS basal medium The 6-BA of / L and the TDZ of 0.001mg / L are formulated into tissue culture medium, are used for inducing the multiplication and the rooting of the mature terminal bud bud tip explant of bamboo pagoda, at the pH value indicated in claim 6 step (2) Under the conditions of temperature, temperature, and light, after subculture 1-2 times, clustered buds will proliferate, and after subculture 2-3 times, roots will take place.

[0019] All the other examples 2-7 can be used for bud tip explants to proliferate and go out clustered buds and rooting, preferably with examples 4 and 7, and examples 5 and 6 are poor. Wherein embodiment 4 is the excellent culture medium that realizes that the multiplication coefficient of in...

Embodiment 8

[0020] Embodiment 8: The present embodiment is the rapid propagation method of the group culture seedling with the shoot tip of the bamboo top bud or the side bud as the explant, and needs the following steps:

[0021] (1) Collection and disinfection of explants: Spray pesticides to kill insects and sterilize bamboo gardens in the field from April to June. After 1-2 weeks of treatment, take terminal buds or side buds and bring them back to the implementation room, rinse them with tap water for 2-3 hours, and then Soak in 75% alcohol for 1 min, treat with 0.5% NaClO and 0.2% Tween by weight for 15 min under vacuum, and rinse with sterile water for 5-6 times. Peel off the sheath slice wrapped around the bud body, and cut off the bud tip with a length of about 5 mm containing the growth point as an explant.

[0022] (2) Induction and proliferation of buds: inoculate the cut bud tips into MS basal medium containing 6-BA2.0-3.5mg / L and TDZ 0.001-0.003mg / L, pH=5.7, at 25± Cultivate...

Embodiment 9

[0025] Embodiment 9: This embodiment is related to the preparation of potted substrates for transplanting to potted plants. Peat, leech, and perlite are mixed in a volume ratio of 1: 1: 1, and then 1 liter of water is added for every 3 liters of substrates. Add 3 grams of streptomycin or oxytetracycline or gentamicin, etc. and mix well. The selected antibiotics should be low-priced and broad-spectrum.

[0026] In the above-mentioned examples, those that do not show specific conditions are operated according to the routine operation or the conditions noted on the commodity packaging.

[0027] In the present application, the multiplication coefficient refers to the ratio of the total number of clustered shoots of test-tube plantlets to the total number of explants producing these clustered shoots.

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Abstract

The invention provides a shoot-tip tissue culture medium for Sasa argenteastriatus, which comprises the following components: 2.0 to 3.5 mg / L of MS culture medium supplemented with 6-BA and 0.001 to 0.003 mg / L of TDZ. The shoot-tip tissue culture medium can induce the growth of buds and roots and realize the rapid propagation and multiplication of regenerated seedlings. The method of tissue culture and rapid propagation using the shoot-tip tissue culture medium comprises the following four steps: explant collection and selection, bud induction and propagation, root induction and acclimatization and transplantation. With the method of tissue culture and rapid propagation using the shoot-tip tissue culture medium, the multiplication coefficient reaches 5.2, the rate of root regeneration in the test tube is as high as 100%, and the survival rate of transplanting test-tube plantlets reaches 95%. The culture medium overcomes the difficulty in tissue culture and plant regeneration of mature bamboo explants, realizes the induction of clustered buds and roots and the cultivation of regenerated plants, reduces the land, maternal plant, time and cost, solves the season limitation on propagation, and lays the foundation for bulk potted landscaping and large-area afforestation of bamboo and rapid and sufficient supply of bamboo seedlings.

Description

【Technical field】 [0001] The invention relates to a tissue culture medium and a tissue culture rapid propagation method of small-diameter mixed-growing bamboo, that is, bamboo pagoda, using the tip of the terminal bud or the bud tip of the side bud as an explant. 【Background technique】 [0002] Bamboo (Sasa argenteostria ta) is a kind of evergreen grass plant, which is a small-diameter mixed bamboo, 0.2-0.4m high, 0.2-0.3cm in diameter, green leaves, with yellow or white vertical stripes in between, it is a bonsai ornamental and excellent bamboo species for slope protection. For a long time, the propagation of pagoda has been realized by transplanting the whole clump of mother bamboo or whip root transplantation, which has the disadvantages of being limited by seasons, large consumption of mother bamboo, large land use, inconvenient transportation of mother bamboo, and low reproduction coefficient. In recent years, the exploration and practice of bamboo tissue culture techn...

Claims

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Application Information

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IPC IPC(8): A01H4/00A01G31/00C12N5/04
CPCY02A40/22Y02P60/40
Inventor 林新春方伟樊琳何冬冬黄丽春
Owner ZHEJIANG FORESTRY UNIVERSITY
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