Chemiluminescence system for determining benzoyl peroxide and method

Abstract

The invention relates to a chemiluminescent system and a method which uses the system to determine benzoyl peroxide in flour. The chemiluminescent system is a mixed reaction system of A solution and B solution; the A solution is a luminescent reagent which contains Luminol of 6.0*10<-5> to 2.5*10<3>mol/L, CO3<2-> of 0.20 to 1.00mol/L and OH<-> of 0.08 to 0.20mol/L; the B solution is detected solution which contains Co<2+> of 1.0*10<-8> to 1.0*10<-6>. The method mainly comprises the following steps: mixing A and B with a volume ratio of from 0.8 to 1.2 to 1 in the detection container of a chemiluminescent instrument, and detecting and reading the luminous intensity of the chemiluminescent reaction. The chemiluminescent system has the advantages of high stability, high sensitivity, moderate reaction velocity, anti-interference performance, and excellent selectivity of the detected matter. The method of operation is simple, time-saving and efficient.

Description

technical field [0001] The invention relates to the field of chemiluminescence analysis, in particular to a chemiluminescence system for determining benzoyl peroxide and a method for determining benzoyl peroxide in flour using the system. Background technique [0002] Benzoyl peroxide (Benzoyl Peroxide, BPO) is a flour additive that can oxidize the pigment in flour to a colorless substance, and transform itself into benzoic acid (BA), thereby increasing the whiteness of flour and improving Flour grades and prices. But both BPO and BA are harmful substances to the human body, especially to the liver. my country's GB2760-1996 clearly stipulates that the content of BPO added to flour must be less than 60mg / kg. [0003] Establishing an accurate, sensitive and rapid method for the determination of BPO in flour is of great significance for the monitoring of flour quality and the protection of people's health. GB / T18415-2001 determined that the determination method of BPO is gas...

AI Technical Summary

Problems solved by technology

At present, most of the determination methods reported in the literature are indirect analysis methods, commonly used are iodometric method, ultraviolet spectrophotometry, gas chromatography, high performance liquid chromatography, electrochemical analysis, etc., all of which require repeated procedures for removing impurities and are cumbersome to operate , the measurement time is long and the equipment is expensive, so it is difficult to popularize

[0004] Wada et al. used benzoic acid peroxide to transform into H under the irradiation of ultraviolet light. 2 o 2 Combined with the chemiluminescence reaction of peroxyoxalate, an indirect chemiluminescent method for measuring benzophthalein peroxide was established, and the detection limit of the method was 6.8umoL; the patent document CN 1444034A discloses the use of benzophthalide peroxide and luminol Due to the characteristics of direct reaction to generate chemiluminescence, a chemiluminescence method of benzophthalein peroxide was established, and the detection limit of this method was 4×10 -7 g / ml; there are other systems such as Luminol-POB-K 4 [Fe(CN) 6 ], GA-POB-HCHO, Luminol-POB-KMnO 4 etc., these systems are for the indirect determination of BPO, through the chemical reaction between BPO and some reagents to determine the remaining reactants or products, as long as the substances in the liquid to be tested can interfere with the determination of chemiluminescent reactions with these reagents, so these methods The selectivity is too poor to be applied to the determination of actual samples

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