Unlock instant, AI-driven research and patent intelligence for your innovation.

Plants cold-tolerant and drought resisting protein, encoding genes and use thereof

A gene-encoded, cold-resistant technology, applied in the field of bioengineering, can solve problems such as not found

Inactive Publication Date: 2009-01-21
HEFEI UNIV OF TECH
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, no effective genes with both drought and cold resistance functions have been found. Facing the increasingly serious drought and cold resistance problems of crops, it is of great theoretical and practical significance to find new genes for drought and cold resistance functions and to clarify their functions.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Plants cold-tolerant and drought resisting protein, encoding genes and use thereof
  • Plants cold-tolerant and drought resisting protein, encoding genes and use thereof
  • Plants cold-tolerant and drought resisting protein, encoding genes and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1, the acquisition of LWT2-1 and its coding gene

[0032] About 50-100 mg of wild-type Colombian ecotype Arabidopsis seedlings were used as materials, the total RNA was extracted with Trizol, and the RNA concentration was detected with an ultraviolet spectrophotometer. Press RevertAid TM First Strand cDNA Synthesis Kit (Fermentas Company) kit manual, using the extracted total RNA as a template to synthesize the first strand of cDNA. Using the extracted first-strand cDNA as a template, perform the following PCR reaction: 20 μl reaction system, containing 2 μl of 10×PCR buffer, 0.4 μl of dNTPs (10 mM) mixture, 2 μl each of primer 1 and primer 2, and Tag enzyme (5 U / μl) 0.2μl, and add double distilled water to the rest to 20μl. Among them, primer 1: 5'-AAAACATCAAAATGGCTGAAAAAG-3', primer 2: 5'-TCATTAAACTGACTGCTGAGAAATG-3'. Amplification on the Life Express gene thermal cycler: first 94°C pre-denaturation for 3min, then 94°C for 30sec, 54°C for 30sec, 72°C for ...

Embodiment 2

[0033] Embodiment 2, cultivating Arabidopsis thaliana with enhanced cold resistance and drought resistance

[0034] 1. Obtaining the homozygous mutant lwt2-1 whose LWT2-1 gene has been knocked out

[0035]T-DNA insertion mutant seeds were obtained from the American Arabidopsis Germplasm Center (SALK_023905; http: / / www.arabidopsis.org / servlets / TairObject?type=germplasm&id=4626493). To identify homozygous mutants with knockout of the LWT2-1 gene, SALK_023905 seeds were sown and DNA was extracted from a single plant, which was used as a template for PCR amplification with 3 pairs of primers. Among them, primer 1 LBb1: 5'-GCGTGGACCGCTTGCTGCAACT-3, primer 2: LWT2-LP 5'-AAGCACAAACGTGGTTACCTG -3', primer 3: LWT2-RP5'-GTCGCTTTGCTATGCTCAAAG-3'. According to the results of agarose gel electrophoresis of PCR products, a homozygous mutant lwt2-1 in which the LWT2-1 gene was knocked out was obtained. From the phenotype point of view, there was no significant difference between the mutant...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a vegetable cold-resistance and drought-resistance protein as well as a coding gene and an application thereof. The vegetable cold-resistance and drought-resistance protein is SEQ ID No:2 in a sequence list, and the coding gene is an SEQ ID No:1 DNA sequence in the sequence list. The invention uses the coding gene of the cold-resistance and drought-resistance protein to enhance the cold-resistance and drought-resistance of vegetable, including the inhibition on the expression of corresponding coding genes of the vegetable cold-resistance and drought-resistance protein in the vegetable, to provide technical support for cultivation of cold-resistance and drought-resistance strains of crops.

Description

1. Technical field [0001] The invention relates to a plant drought resistance and cold resistance related protein and its coding gene and application in the field of bioengineering, in particular to a method for enhancing plant drought resistance and cold resistance by using the gene. 2. Background technology [0002] The food problem is one of the major problems facing the world today. Increasing grain production by increasing unit yield or expanding planting area, increasing agricultural input to transform low-yielding fields, etc. will encounter the problem of overcoming adversity constraints or mitigating adversity hazards. Therefore, understanding the response mechanism of plants to adversity and improving the stress resistance of plants has become an important basic research for further increasing agricultural production. [0003] Low temperature and drought are two important adversity factors that affect and limit crop production, causing significant losses to produc...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C07K14/415C12N15/29A01H1/00A01H5/00
Inventor 曹树青陈正义江力姜绍通孔维文边小会简红勇
Owner HEFEI UNIV OF TECH