Use of Liriope muscari Bailey C in pharmacy

A technology of Ophiopogon japonicus and inhibitors, which is applied in the field of pharmaceutical application of Ophiopogon japonicus C, can solve the problems that the survival period cannot be better extended and has not been reported.

Active Publication Date: 2009-01-28
TIANJIN TASLY PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Once cancer patients have metastases, even with surgery, radiotherapy, and chemotherapy, the survival time cannot be pr

Method used

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  • Use of Liriope muscari Bailey C in pharmacy
  • Use of Liriope muscari Bailey C in pharmacy
  • Use of Liriope muscari Bailey C in pharmacy

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Example 1 Detection of toxicity of DT-13 to cells

[0017] (1) Material:

[0018] Instrument: Ultra-clean workbench (Suzhou Aikelin Purification Equipment Co., Ltd.), constant temperature CO 2 Incubator (Heraeus, Germany), enzyme-linked immunoassay (BIO-RAD, USA), inverted biological microscope (OLYMPUS, Japan), plate shaker (Jiangsu Guangming Experimental Instrument Factory).

[0019] Reagents: RPMI1640, F12 medium (GIBCO), trypsin (SIGMA), newborn calf serum (HYCLONE), MTT (SIGMA), DMSO (SIGMA).

[0020] Cell lines: human breast cancer cells MDA-MB-435, MDA-MB-231, MCF-7.

[0021] (2) Method:

[0022] 1. Take a bottle of cells that are in good condition in the exponential growth phase, add 0.25% trypsin digestion solution, digest to shed adherent cells, and count 2~4×10 4 Pcs / ml to make cell suspension.

[0023] 2. Inoculate the cell suspension on a 96-well plate, 180μl / well, and place constant temperature CO 2 Incubate in an incubator for 24 hours.

[0024] 3. Change the...

Embodiment 2D

[0036] Example 2 The effect of DT-13 on cell adhesion in hypoxic state

[0037] OBJECTIVE: To detect changes in the ability of human breast cancer cells to adhere to basement membrane under hypoxia in vitro

[0038] Materials: Six-well plate, 96-well plate, PBS, 1% gelatin, 2% BSA, serum-free medium, 10% calf serum medium, digestion solution (0.25% trypsin), MTT reagent, DMSO

[0039] method:

[0040] 1. Preparation of Gelatin Board:

[0041] 1. Weigh a certain amount of gelatin powder, add 1g / 100ml amount of hot PBS to dissolve it, take advantage of the hot spot into a 96-well plate, 30μl / well, lay flat and shake to make the gelatin flatten the bottom of the well, be careful not to have bubbles .

[0042] 2. Leave it overnight, suck out the gelatin, and wash it with PBS.

[0043] 3. Block it with 2% BSA for 1 hour and then suck it off.

[0044] 4. Add serum-free medium, 20-30μl / well, equilibrate for more than 1 hour, and then aspirate.

[0045] Second, cell culture

[0046] 1. Vi...

Embodiment 3

[0071] Example 3 The effect of DT-13 on cell migration ability in hypoxic state

[0072] Use Boyden Chamber Transwell to detect cell migration ability. The device is divided into internal and external rooms. The bottom of the inner chamber is a polycarbonate filter membrane (pore size 8 μm), which is first coated with 1% gelatin, and then equilibrated with serum-free culture medium for 1 hour. Human breast cancer cells (1.5×10 4 Each cell / 100μl) was suspended in a culture medium containing 2% FBS and different concentrations of the test compound and seeded into the inner chamber. Add 0.6ml of culture medium with or without VEGF (20ng / ml) to the outer chamber. After incubating at 37°C for 6 hours, discard the culture medium in the wells and fix the cells with 90% ethanol for 10 minutes. Use a cotton swab to gently wipe away the non-migrated cells in the upper layer of the inner chamber, stain with 0.1% crystal violet at room temperature for 10 minutes, rinse the excess dye with PBS...

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Abstract

The invention belongs to the field of pharmacy, and discloses the application of rusogenin fucopyranoside C in the pharmacy. The invention also provides the application of the rusogenin fucopyranoside C in the preparation of the medicine for resisting tumor metastasis, and more particularly relates to the application in the preparation of the medicine for generating tumour new blood vessels and inhibition of relative tumor metastasis. Being proved by the study of the invention, the rusogenin fucopyranoside C can effectively inhibit the growth, migration and adhesive ability, can inhibit lung metastasis of melanoma B16, and provides new selection in the application of preparing the medicine for resisting tumor metastasis.

Description

Technical field [0001] The invention belongs to the field of pharmacy, and relates to the application of Ophiopogon japonicus C in pharmacy. Background technique [0002] Liriope muscari (Decne.) Baily, as one of the original plants of the newly added varieties of "Chinese Pharmacopoeia" 1995 edition of Ophiopogon japonicus, has shown its unique curative effect in the long-term clinical practice of traditional Chinese medicine. However, the clinical application of Ophiopogon japonicus is still focused on decoction pieces or compound preparations, such as Shengmaisan, Shenmai injection, etc. So far, there has been no single use of its extract or monomer compound as a clinical drug. Therefore, it is of great significance to carry out in-depth research and development. [0003] In terms of chemical composition research, the main focus is on the separation and identification of the saponins of Ophiopogon japonicus. Since the total saponins content in the fibrous roots of Ophiopogon j...

Claims

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Application Information

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IPC IPC(8): A61K31/7048A61P35/00
Inventor 袁胜涛孙立余伯阳张陆勇林森森朱丹妮施伟
Owner TIANJIN TASLY PHARMA CO LTD
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