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High yield liquid fermentation process of Agrocybe chaxingu Huang mycelia of polysaccharide and triterpene substance

A technology of liquid fermentation and tea mushroom, applied in the field of bioengineering, can solve the problem of low physiologically active substances and achieve the effect of reducing production costs

Inactive Publication Date: 2010-12-29
LUDONG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] There are also some relevant literatures reporting the method of liquid fermentation of Aphrodisiac agaricus, but its main physiologically active substances are relatively low. So far there is no mature liquid fermentation method for high-yield Acacia acuminosa polysaccharides and triterpenoids. Therefore, it is necessary to develop and research as soon as possible. A technology that can simultaneously produce high-yielding polysaccharides and triterpenoids through liquid fermentation to achieve the goal of "two medicines in one pot" is urgently needed by people

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] (1) Activation and cultivation of slant strains: Inoculate the slender mushroom strains on the PDA slant medium and activate them, then transfer them to the PDA plate medium to cultivate the slant strains. The culture temperature is 27°C and the culture time is 7 days. .

[0027] (2) Preparation of liquid strains:

[0028] Liquid medium composition: potato 200g / L, glucose 20g / L, yeast extract 2g / L, the balance is distilled water; the pH value is natural.

[0029] Culture conditions: Pick 3 pieces of activated slant strains, inoculate them in a 500mL Erlenmeyer flask (containing 100mL of liquid medium), and culture them in a shaker with a rotation speed of 150r / min and a temperature of 27°C for 7 days.

[0030] (3) Liquid fermentation culture:

[0031] Fermentation medium composition: glucose 16g / L, peptone 2.93g / L, corn flour 20.93g / L, soybean flour 6.44g / L, and distilled water as the balance.

[0032] Culture conditions: Inoculate the prepared liquid bacteria into a...

Embodiment 2

[0037] (1) Activation and cultivation of slant strains: inoculate the Aphrodisiac edulis strains on the PDA slant medium and activate them, then transfer them to the PDA plate medium to cultivate the slant strains, the culture temperature is 25°C, and the culture time is 10 days .

[0038] (2) Preparation of liquid strains:

[0039] Liquid medium composition: potato 150g / L, glucose 15g / L, yeast extract 1g / L, the balance is distilled water; the pH value is natural.

[0040] Culture conditions: Pick 2 pieces of activated slant strains, inoculate them in a 500mL Erlenmeyer flask (containing 50mL of liquid medium), and culture them in a shaker with a rotation speed of 100r / min and a temperature of 25°C for 8 days.

[0041] (3) Liquid fermentation culture:

[0042] Fermentation medium composition: glucose 10g / L, peptone 1g / L, corn flour 15g / L, soybean flour 2g / L, and distilled water as the balance.

[0043] Culture conditions: Inoculate the prepared liquid bacteria into a 500mL ...

Embodiment 3

[0048] (1) Activation and cultivation of strains on slant: inoculate the strains of Salmonella edulis on the PDA slant medium for activation, then transfer to the PDA plate medium to cultivate the strains on the slant, the culture temperature is 30°C, and the culture time is 5 days .

[0049] (2) Preparation of liquid strains:

[0050] Liquid medium composition: potato 250g / L, glucose 25g / L, yeast extract 5g / L, the balance is distilled water; the pH value is natural.

[0051] Culture conditions: Pick 4 pieces of activated slant strains, inoculate them in a 500mL Erlenmeyer flask (containing 200mL of liquid medium), and culture them in a shaker with a rotation speed of 180r / min and a temperature of 30°C for 5 days.

[0052] (3) Liquid fermentation culture:

[0053] Fermentation medium composition: glucose 16g / L, peptone 2.93g / L, corn flour 20.93g / L, soybean flour 6.44g / L, and distilled water as the balance.

[0054] Culture conditions: Inoculate the prepared liquid bacteria in...

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Abstract

The invention relates to a liquid fermentation technology of Agrocybe aegerita mycelia of high yielding polysaccharides and triterpenes materials. The technology comprises the steps of inclined strain activation culture, liquid strain preparation, and liquid fermentation culture; the technology adopts glucose and corn flour as a carbon source, and adopts a peptone and soybean meal as a nitrogen source so as to carry out the production by adopting a liquid aerobic fermentation method, therefore, the Agrocybe aegerita polysaccharides and triterpenes materials can be obtained simultaneously, wherein, the yield of the Agrocybe aegerita polysaccharides and triterpenes materials can reach more than 2.10g / L and more than 842.51mg / L respectively. The technology is simple and can achieve the aim of 'two drugs in one tin', thus being a production method with great industrial prospect.

Description

Technical field: [0001] The invention relates to a liquid fermentation process for producing polysaccharides and triterpenoids by using the edible fungus Aphrodisiac aleaves, which belongs to the technical field of bioengineering and uses the cultivation and fermentation of organisms to produce known or new organisms, or technology of its metabolites. Background technique: [0002] Agrocybe aegerita belongs to Basidiomycetes, Agrocybe aegeritaceae and Agrocybe genus. It is a rare edible and medicinal fungus with high development value. It has a unique taste and rich nutrition. It has the special functions of removing dampness, diuresis, invigorating the spleen and stomach, improving eyesight, anti-aging, lowering cholesterol, preventing and fighting cancer, and can also treat dizziness, headache, diarrhea, and vomiting. Tea Salmonella has been extensively cultivated in China in recent years, but due to the disadvantages of long cycle, high cost, and great influence on envi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/04C12P33/00C12N1/14C12R1/645
Inventor 高兴喜王玥程显好王淑芳张桂春李杏伟
Owner LUDONG UNIVERSITY