Method for detecting colimycin and special ELISA kit thereof

An enzyme-linked immunosorbent reagent and colistin technology, which is applied in the field of colistin detection, can solve the problems that the detection sensitivity and detection limit cannot be achieved by using instrumental methods, poor thermal stability, and should not be used, so as to shorten the detection time and cost Low cost and low pre-treatment requirements
CN101358966BInactive Publication Date: 2012-11-28BEIJING WANGER BIOTECH
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
BEIJING WANGER BIOTECH
Publication Date
2012-11-28
Estimated Expiration
Not applicable · inactive patent

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Abstract

The present invention discloses a method of detecting colistin, and a special enzyme linked immunosorbent kit thereof. The enzyme linked immunosorbent kit comprises the colistin and specific antibodies of the colistin; and the specific antibodies are polyclonal antibodies or monoclonal antibodies of the colistin. The kit adopts the high specificity monoclonal antibodies of the colistin, which guarantees the reliability of detection results, and the experimental results show that the kit has the characteristics of high specificity, high sensitivity, high precision, high accuracy and the like; and the main reagents of the kit all adopt the form of working liquid, the operation is convenient, and the cost is low. The operation of the method which adopts the kit to detect the colistin is simple, the detection time is shortened, the operation have low requirements for the pretreatment of the sample, and the method can be used for simultaneously and rapidly detecting the samples in bulk. Therefore, the method which adopts the enzyme linked immunosorbent kit for detection can play a significant role in the detection of the colistin.
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Description

technical field

[0001] The invention relates to a method for detecting colistin and a special ELISA kit. Background technique

[0002] Colistin (CLS), also known as polymyxin E (polymyxin E), is produced by multiple colistin (B.polymyxa). It has a strong inhibitory effect on Gram-negative bacteria and can treat infections caused by Shigella dysenteriae, Escherichia coli, Pseudomonas aeruginosa, Salmonella and Proteus vulgaris, and it can also be used as a feed additive, Improve the feed conversion rate, promote the growth of livestock and poultry, and prevent the occurrence of livestock and poultry diseases in large-scale farming. Because colistin sulfate has the characteristics of strong antibacterial effect, low residue, and not easy to produce drug resistance, many countries and regions such as China, the United States, the European Union and Japan have approved it as a feed additive or veterinary drug.

[0003] With the increasing application of colistin sulfate in the...

Claims

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