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Application of Raloxifene compounds in preparing medicament for preventing and controlling benign prostatahyperplasie

A technology for benign prostatic hyperplasia and raloxifene, applied in drug combinations, pharmaceutical formulations, organic active ingredients, etc., can solve problems such as no raloxifene compounds

Inactive Publication Date: 2009-02-18
NANKAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far, there is no research report that raloxifene compounds can prevent and treat benign prostatic hyperplasia

Method used

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  • Application of Raloxifene compounds in preparing medicament for preventing and controlling benign prostatahyperplasie
  • Application of Raloxifene compounds in preparing medicament for preventing and controlling benign prostatahyperplasie
  • Application of Raloxifene compounds in preparing medicament for preventing and controlling benign prostatahyperplasie

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Example 1 MTT method to detect the effect of raloxifene hydrochloride on the proliferation of prostatic stromal cells induced by estradiol

[0015] The prostatic mesenchymal cell line WPMY-1 cultured in vitro was placed in a 24-well plate at a density of 2×10 4 cells / 0.5mL culture medium. at 37°C CO 2 cultured in an incubator. After 24 hours of cell attachment, add 500ul containing 10 -6 M, 10 -7 M and 10 -8 M of raloxifene hydrochloride and 10 -7 Culture medium of M estradiol (E2). 48 hours after drug addition, 100 μL of MTT solution was added to each well. Place the culture plate with MTT solution at 37°C, CO 2 Incubate for 2 hours in the incubator. A purple precipitate is visible under the microscope. Carefully aspirate the medium. Add 0.2mL DMSO solution to each well, shake gently to fully dissolve. The culture solution in each well was sucked into a special 96-well plate for a microplate reader, and the absorbance A570nm was measured. The experimental ...

Embodiment 2

[0020] Example 2 Effect of Raloxifene Hydrochloride on Experimental Animal Model of Prostatic Stromal Hyperplasia in Estrogen / Androgen-Induced Castrated Rats

[0021] Raloxifene hydrochloride (Lilly Suzhou Pharmaceutical Co., Ltd.), estrogen solution formula is testosterone propionate injection (Tianjin Jinyao Amino Acid Co., Ltd.), estradiol benzoate injection (Tianjin Jinyao Amino Acid Co., Ltd.), and Corn oil was used to prepare a mixed solution with an estrogen / androgen ratio of 1:100, and the concentration of estradiol benzoate injection was fixed at 100 μg / mL.

[0022] Forty male Wistar rats were adaptively fed for one week. Rats were randomly divided into 5 groups, with 8 animals in each group, which were normal group, estrogen / androgen alone group, estrogen / androgen+low concentration raloxifene hydrochloride group, estrogen / androgen+medium concentration Raloxifene hydrochloride group and estrogen / androgen + high concentration raloxifene hydrochloride group.

[0023] ...

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Abstract

The invention belongs to the medicinal field, and in particular discloses application of a raloxifene compound in preparing medicines for preventing and treating nonmalignant prostate hyperplasia. As verified by cell and animal model experiments, the raloxifene compound has obvious inhibition effect on proliferation of prostate interstitial cells, can resist the proliferation promoting effect of estradiol on the interstitial cells, and has obvious effect of treating and improving the pathologic change that estrogen / androgen induces prostate interstitial proliferation of an emasculated mouse.

Description

【Technical field】 [0001] The invention relates to the application of raloxifene compounds in the preparation of medicines for preventing and treating benign prostatic hyperplasia. It belongs to the field of medicine. 【Background technique】 [0002] Benign prostatic hyperplasia (BPH) is one of the most common urological diseases in elderly men. Its histological features are generally divided into 5 types, namely, stromal hyperplasia, fibromuscular hyperplasia, muscle hyperplasia, fibroadenomatous hyperplasia, and fibromuscular adenomatous hyperplasia. Among them, fibromuscular adenoma hyperplasia is the most common. [0003] Over the years, the medical community has consistently recognized that old age and functioning testicles are the two major factors leading to BPH. In recent years, more and more studies have shown that the ratio of estrogen / androgen in the body increases, and the enhancement of estrogen effect participates in the homeostasis of the prostate gland and t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/4535A61P13/08
Inventor 张琚石建党杨睿杜小玲马洪顺杨占坡
Owner NANKAI UNIV
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