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Immune globulin A detection reagent

A technology of immunoglobulin and detection reagents, which is applied in the direction of biological testing, material inspection products, etc., can solve the problems of cumbersome operation and inability to analyze and detect batch samples, and achieve the effect of good repeatability, high accuracy and simple operation

Inactive Publication Date: 2009-02-18
王贤理
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Known methods for determining immunoglobulin A (IgA) include immunodiffusion method, immunoelectrophoresis method, and radioimmunoassay method. These methods are cumbersome to operate, require special equipment, and require pretreatment of samples, which cannot be used for batch sample analysis and Disadvantages such as not being able to be directly tested by a fully automatic biochemical analyzer

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] 1. IgA reagent (R1)

[0059] Phosphate (buffer) 100mmol / L

[0060] Polyoxyethylene lauryl ether (surfactant) 5%

[0061] NaCl (electrolyte) 10%

[0062] PEG-6000 (polymer accelerator) 4%

[0063] Broad-spectrum fungicide (preservative) 0.1%

[0064] Hexadimethylamine bromide (reaction accelerator) 0.3%

[0065] Disodium EDTA (stabilizer) 0.05%

[0066] 2. Anti-IgA antibody reagent (R2)

[0067] Phosphate (buffer) 100mmol / L

[0068] Polyoxyethylene lauryl ether (surfactant) 5%

[0069] NaCl (electrolyte) 10%

[0070] PEG-6000 (polymer accelerator) 4%

[0071] Broad-spectrum fungicide (preservative) 0.4%

[0072] Disodium EDTA (stabilizer) 0.05%

[0073] Rabbit anti-human IgA antiserum 10%

[0074] Butylated hydroxyanisole (antioxidant) 0.01%

[0075] Mannitol (stabilizer) 5%

[0076] 3. Liquid serotype IgA constant value calibrator

[0077] CAPSO (buffer) 100mmol / L

[0078] Broad-spectrum fungicide (preservative) 0.01%

[0079] Disodium edetate (stabiliz...

Embodiment 2

[0084] 1. IgA reagent (R1)

[0085] Phosphate (buffer) 5mmol / L

[0086] Polyoxyethylene lauryl ether (surfactant) 0.1%

[0087] NaCl (electrolyte) 0.1%

[0088] PEG-6000 (polymer accelerator) 7%

[0089] Broad-spectrum fungicide (preservative) 0.01%

[0090] Polybrene (reaction accelerator) 0.1%

[0091] Disodium EDTA (stabilizer) 1.0%

[0092] 2. Anti-IgA antibody reagent (R2)

[0093] Phosphate (buffer) 5mmol / L

[0094] Polyoxyethylene lauryl ether (surfactant) 0.1%

[0095] NaCl (electrolyte) 0.1%

[0096] PEG-6000 (polymer accelerator) 7%

[0097] Broad-spectrum fungicide (preservative) 0.01%

[0098] Disodium EDTA (stabilizer) 2%

[0099] Goat anti-human IgA antiserum 50%

[0100] Butylated hydroxyanisole (antioxidant) 0.5%

[0101] Magnesium chloride (stabilizer) 1%

[0102] 3. Liquid serotype constant value IgA calibrator

[0103] CAPSO (buffer) 20mmol / L

[0104] Broad-spectrum fungicide (preservative) 1%

[0105] Glycerol (stabilizer) 1%

[0106] Bovi...

Embodiment 3

[0110] 1. IgA reagent (R1)

[0111] Phosphate (buffer) 200mmol / L

[0112] Polyoxyethylene lauryl ether (surfactant) 10%

[0113] NaCl (electrolyte) 5%

[0114] PEG-6000 (polymer accelerator) 2%

[0115]Broad-spectrum fungicide (preservative) 1%

[0116] Polybrene (reaction accelerator) 0.5%

[0117] Disodium edetate (stabilizer) 1%

[0118] 2. Anti-IgA antibody reagent (R2)

[0119] Phosphate (buffer) 200mmol / L

[0120] Polyoxyethylene lauryl ether (surfactant) 10%

[0121] NaCl (electrolyte) 5%

[0122] PEG-6000 (polymer accelerator) 2%

[0123] Broad-spectrum fungicide (preservative) 1%

[0124] Disodium EDTA (stabilizer) 0.05%

[0125] Horse anti-human IgA antiserum 20%

[0126] Butylated hydroxytoluene (antioxidant) 0.3%

[0127] Glycerol (stabilizer) 10%

[0128] 3. Liquid serotype constant value IgA calibrator

[0129] CAPSO (buffer) 100mmol / L

[0130] Broad-spectrum fungicide (preservative) 0.06%

[0131] Disodium edetate (stabilizer) 0.5%

[0132] Bov...

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Abstract

The invention discloses an immunoglobulin A detection reagent, comprising an IgA reagent, an anti-IgA antibody reagent and a liquid serotype constant value calibration agent; wherein, the IgA reagent enables the IgA antigenic sites in the sample to be fully exposed so as to facilitate the full combination with the anti-IgA antibody reagent; the anti-IgA antibody reagent has high idiosyncrasy with the IgA antigens in human serum; and the liquid serotype constant value calibration agent is compared with the sample for result calculation. The anti-IgA antibodies are from sheep, horses, rats or rabbits and other mammals. The IgA reagent and the anti-IgA antibody reagent can be used as two separate reagents to compose the double-reagent form of the product, and can also be mixed based on a certain percentage to constitute the single-reagent form of the product.

Description

technical field [0001] The invention relates to a reagent combination for measuring human body fluid components, in particular to a detection reagent for measuring immunoglobulin A (IgA) in human serum, which can be widely used in the technical fields of medicine and biochemistry. Background technique [0002] Immunoglobulins are a group of proteins with antibody activity, mainly found in plasma, but also in other body fluids, tissues and some secretions. Most of the immunoglobulins in human plasma exist in gamma globulin (γ-globulin). Can be divided into five classes, namely immunoglobulin G (IgG), immunoglobulin A (IgA), immunoglobulin M (IgM), immunoglobulin D (IgD) and immunoglobulin E (IgE). The content of IgA in normal human serum is second only to IgG, accounting for 10-20% of serum immunoglobulin content. From the structural point of view, IgA can be divided into monomer, dibody, tribody and multimer. According to its immune function, it is divided into two types:...

Claims

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Application Information

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IPC IPC(8): G01N33/53
Inventor 王贤理蒙凯蔡其浩
Owner 王贤理
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