Chemical luminescence immune analysis quantitative measuring reagent kit for IV type collagen and preparation method thereof

Abstract

The invention relates to the immunological analysis medical field, and in particularly provides a chemiluminescence immunoassay quantitative analysis test kit and the preparing method thereof for type IV collagen protein correlated to liver fibrosis degree. The test kit comprises: 1) type IV collagen protein calibrating article; 2) solid-phase carrier coated by avidin; 3) antibody enzyme-labeled compound of the type IV collagen protein; 4) biotinylated type IV collagen protein antibody; 5) chemiluminescence zymolyte liquid; and 6) condensed washing liquid. Furthermore, the method of preparing the test kit comprises the following steps: 1) the calibrating article is prepared by using pure type IV collagen protein; 2) the solid-phase carrier is coated by the avidin; 3) the antibody of the type IV collagen protein is labeled by enzyme; 4) the antibody of the type IV collagen protein is biotinylated; 5) the chemiluminescence zymolyte liquid is prepared; 6) the condensed washing liquid is prepared; 7) the calibrating article, the labeled compound, the chemiluminescence zymolyte liquid and the washing liquid are filled and packed through separate packing; and 8) the finished product is assembled. The test kit has the advantages of simplicity, swiftness, sensitivity, stability and the like.

Description

technical field [0001] The invention relates to the field of immunoanalysis medicine, in particular, the invention provides an early detection index IV collagen chemiluminescence immunoassay quantitative assay kit for liver fibrosis and a preparation method thereof. Background technique [0002] The liver is the largest digestive gland in the human digestive system and an important organ of metabolism. Hepatic fibrosis refers to the excessive deposition of fibrous connective tissue in the liver, which is the result of unbalanced fibrosis and fibrolysis. Hepatic fatty changes, cholestasis, and inflammation caused by various reasons can stimulate local hepatic fibrosis to a certain degree. The fat storage cells and resting fibroblasts proliferate and form collagen fibers, and the increased fibrous tissue forms fine cords and thin intervals, which causes liver fibrosis and leads to a syndrome of impaired liver function. Hepatic fibrosis is the early stage of liver cirrhosis. E...

AI Technical Summary

Problems solved by technology

Radioimmunoassay has problems such as complex operation, unstable measurement results, short storage time of reagents, radioactive contamination, and expensive instruments; while enzyme-linked immunoassay has disadvantages such as low sensitivity and narrow linear range

Images