Technique for preparing low chitose and chitosan oligosaccharide

A technology of chitosan oligosaccharide and low chitosan, which is applied in the field of biological enzymatic production of low chitosan and chitosan oligosaccharide, can solve the problems of poor uniformity of degradation products and low degradation efficiency, and achieve low comprehensive production cost and high degradation efficiency , To ensure the effect of stable product quality

Inactive Publication Date: 2009-03-11
YANGZHOU RIXING BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But this kind of non-specificity enzyme is used for the degradation of chitosan, and the main problem that exists is that degradation efficiency is not high, as adopting cellulase to carry out chitosan degradation, the required enzyme amount is about 10% of substrate, adopts When prot

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Add 10kg of chitosan with a deacetylation degree of 95% into the stirring device, add 90kg of water and 5.0kg of acetic acid, stir for 20 minutes, heat up to 60°C, add 300g of cellulose compound enzyme, and react for 3h. Control the reaction temperature at 45°C and add 200g chitosanase for enzymatic hydrolysis for 8h. After the reaction, NaOH solution was used to adjust the pH of the hydrolyzed solution to 7.5, so that chitosan with relatively large molecular weight was precipitated from the solution, left to stand for 4 hours, and the precipitate was removed by vacuum filtration. The filtrate is filtered with an ultrafiltration membrane with a molecular weight cut-off of 10000D, and then subjected to 2000D ultrafiltration, and the filtrate is subjected to 200D nanofiltration to separate salt and conduct preliminary concentration. The concentrated solution was spray-dried to obtain 8.6 kg of chitosan oligosaccharides, the air inlet temperature was 180° C., and the air o...

Embodiment 2

[0027] Add 10kg of chitosan with a deacetylation degree greater than 75% into the stirring device, add 60kg of water and 20kg of hydrochloric acid, stir for 30 minutes, heat up to 50°C, add 500g of protease, and react for 0.5h. Then add 100g chitosanase and react for 0.5h. After the reaction, the pH of the hydrolyzed solution was adjusted to 5.5 with NaOH solution, then allowed to stand for 4 hours, and the precipitate was removed by plate and frame filtration. The filtrate passes through a membrane with a molecular weight cut-off of 3000D, and then undergoes 3000D ultrafiltration. The ultrafiltration concentrate was spray-dried to obtain 5.2 kg of low-molecular-weight chitosan. The ultrafiltration permeate containing oligochitosan was concentrated by 200D nanofiltration, and then spray-dried to obtain 2.4 kg of oligochitosan.

Embodiment 3

[0029] Add 10kg of chitosan with a deacetylation degree of 90% into the stirring device, add 200kg of water and 2.0kg of hydrochloric acid, stir for 40 minutes, heat up to 55°C, add 200g of lipase and 200g of protease, and react for 3 hours. Then adjust the reaction temperature to 60° C., add 200 g chitosanase, and stir for 8.0 h. After the reaction, the pH of the hydrolyzed solution was adjusted to 7.0 with NaOH solution and allowed to stand for 4 hours, and the precipitate was removed by vacuum filtration. The filtrate is filtered with an ultrafiltration membrane with a molecular weight cut-off of 10000D, and then subjected to a 2000D ultrafiltration. Its ultra-filtered concentrate is incorporated into the next batch for processing and added to the reactor during the enzymatic hydrolysis step of chitosan. The ultrafiltration permeate containing the chitosan oligosaccharide component was subjected to 300D nanofiltration and desalination, and spray-dried to obtain 7.5 kg chit...

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Abstract

The invention discloses a technique for preparing oligosaccharide and Chitosan oligosaccharide. The technique takes the Chitosan oligosaccharide with the deacetylation degree between 70 and 95 percent as a raw material; and the oligosaccharide and the Chitosan oligosaccharide are prepared through the steps of dissolution, quick viscosity reduction of a non-specific enzyme, hydrolysis of chitoanase, adjustment of the pH of digest to between 5.5 and 7.5, filtration, film-method refining, spray drying and so on. The technique has the advantages of the enzyme preparation technique, and simultaneously has the characteristics of high degradation efficiency and transformation efficiency and low cost.

Description

technical field [0001] The invention relates to a process for producing low chitosan and chitosan oligosaccharide by biological enzyme method. Background technique [0002] Low-molecular chitosan (low chitosan) is a degradation product of chitosan. It is generally believed that its molecular weight is less than 10,000, and low-molecular chitosan below 2,000 is also called chitosan oligosaccharide, which is generally composed of 2 to 10 sugars. Basic composition. As a new class of physiologically active substances, low chitosan and chitosan oligosaccharides not only have the effect of enhancing immune function, but also have antiseptic and antibacterial effects, improve intestinal tract, moisturize and beautify, etc. Their application and development fields are very broad, and the main application fields are as follows . [0003] (1) Medicine and health products [0004] Oligochitosan can be used as a drug for the treatment of early tumors. It has become a hot spot in dome...

Claims

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Application Information

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IPC IPC(8): C12P19/14C12P19/04C12P19/00
Inventor 张卫明史劲松孙达峰戚善龙张超张和
Owner YANGZHOU RIXING BIO TECH
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