Method for extracting inulin with assistance of biological enzyme

An auxiliary extraction and bio-enzyme technology, applied in food science, food preparation, application, etc., can solve the problems of high energy consumption, long extraction time, and low extraction rate in water bath extraction, and achieve low cost, reduced energy consumption, The effect of increasing the extraction rate

Inactive Publication Date: 2009-04-08
FENCHEM BIOTEK
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the disadvantages of low extraction rate, high energy consumption and long extraction time in the prior art, the present invention provides a

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Using Jerusalem artichoke as raw material, the steps of bio-enzyme-assisted extraction are as follows:

[0029] (1) Wash and slice the fresh Jerusalem artichoke rhizomes, the thickness of the slices is about 0.5cm. Hot water blanching heat treatment was carried out in 80°C hot water for 10 minutes, and the polyphenol oxidase in Jerusalem artichoke was inactivated, and then dried at 50°C for 4 hours to make dried Jerusalem artichoke slices.

[0030] (2) The dried Jerusalem artichoke flakes are pulverized into dried Jerusalem artichoke powder, and the pulverized particle size is 60 mesh. The dried Jerusalem artichoke powder is soaked with HAc-NaAc buffer solution with a pH value of 4.8, and the amount of the buffer solution is used to completely soak the dry Jerusalem artichoke powder. The soaking temperature is 50°C, and the soaking time is 0.5h. And 0.2% cellulase and pectinase are added therein, and the addition ratio is 1:1, so as to facilitate cell disruption and m...

Embodiment 2

[0038] Using Jerusalem artichoke as raw material, the steps of bio-enzyme-assisted extraction are as follows:

[0039] (1) Wash and slice the fresh Jerusalem artichoke rhizomes, the thickness of the slices is about 0.5cm. Hot water blanching heat treatment was carried out in 80°C hot water for 10 minutes, and the polyphenol oxidase in Jerusalem artichoke was inactivated, and then dried at 50°C for 4 hours to make dried Jerusalem artichoke slices.

[0040](2) The dried Jerusalem artichoke flakes are pulverized into dried Jerusalem artichoke powder, and the pulverized particle size is 60 mesh. The dried Jerusalem artichoke powder is soaked with HAc-NaAc buffer solution with a pH value of 5.2, and the amount of the buffer solution is used to completely soak the dry Jerusalem artichoke powder. The soaking temperature is 45°C, and the soaking time is 0.5h. And 0.1% cellulase and pectinase are added therein, and the ratio of addition is 1:2, so as to facilitate cell disruption and...

Embodiment 3

[0048] Using Jerusalem artichoke as raw material, the steps of bio-enzyme-assisted extraction are as follows:

[0049] (1) Wash and slice the fresh Jerusalem artichoke rhizomes, the thickness of the slices is about 0.5cm. Hot water blanching heat treatment was carried out in 80°C hot water for 10 minutes, and the polyphenol oxidase in Jerusalem artichoke was inactivated, and then dried at 50°C for 4 hours to make dried Jerusalem artichoke slices.

[0050] (2) The dried Jerusalem artichoke flakes are pulverized into dried Jerusalem artichoke powder, and the pulverized particle size is 60 mesh. The dried Jerusalem artichoke powder is soaked with HAc-NaAc buffer solution with a pH value of 4, and the amount of the buffer solution is used to completely soak the dry Jerusalem artichoke powder. The soaking temperature is 40°C, and the soaking time is 20 minutes. And 0.15% cellulase and pectinase are added therein, and the ratio of addition is 1:3, so as to facilitate cell disrupti...

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PUM

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Abstract

The invention discloses a method for biological enzyme-assisted extraction of inulin, the hot water extraction method is used for extracting Jerusalem artichoke dry powder after the pre-treatment to obtain inulin crude extraction liquid; the decolorization and the impurity removal are carried out, an ultrafiltration membrane is used for purifying the inulin liquid, and the inulin is finally obtained by vacuum concentration and spray drying, HAc-NaAc buffer solution with pH value of 4-5.5 is used for immersing the Jerusalem artichoke dry powder before the hot water extraction, wherein, 0.1 wt percent-0.5 wt percent of cellulase and pectinase are added, the adding proportion of the cellulase to the pectinase is 1:1-3:1 by weight, the immersion temperature is 40 DEG C-55 DEG C, and the time is 0.5-1.5h. The shortcomings of low extraction rate, high water bath extraction power consumption and long extraction time in the prior art are solved. The invention provides the method for the biological enzyme-assisted extraction of the inulin, and the method has the advantages of high extraction rate and short water bath extraction time.

Description

technical field [0001] The invention relates to a method for extracting inulin, in particular to a method for assisting the extraction of inulin with biological enzymes. Background technique: [0002] Inulin is a linear polysaccharide formed by connecting D-fructose through β-(2,1) glycosidic bonds, and often contains a glucose group at the end. Its degree of polymerization (DP) is about 2-60. Inulin is a non-digestible carbohydrate that selectively promotes the growth of colonic bacteria and improves the health of the host. Moreover, inulin can also lower blood sugar concentration, maintain the balance of lipid metabolism, improve the bioavailability of mineral elements, and enhance immunity. In the food industry, inulin can improve food texture, improve rheological properties and nutritional properties, and is a functional food. As a natural functional food ingredient, inulin has been widely used in European and American countries. [0003] my country has only entered ...

Claims

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Application Information

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IPC IPC(8): C12P19/14C08B37/00A23L29/00
CPCC08B37/18C12P19/04A23L1/03C12P19/14C08B37/0054A23L1/3081A23L33/22
Inventor 范淑峰刘娟王卓
Owner FENCHEM BIOTEK
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