Bacillus licheniformis and use thereof

A technology of Bacillus licheniformis and bacterial agents, applied in the direction of bacteria, mining fluids, microorganisms, etc., can solve problems such as no research rules

Active Publication Date: 2009-04-22
DAQING OILFIELD CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the exploration and research of using microbial/chemical flooding to further enhance oil recovery after polymer flooding is still a relatively cutting-e...

Method used

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  • Bacillus licheniformis and use thereof
  • Bacillus licheniformis and use thereof
  • Bacillus licheniformis and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1. Screening, cultivation and preservation of Bacillus licheniformis U1-3 CGMCC No. 2437

[0039] Bacterial enrichment medium: K 2 HPO 4 0.1-0.5%, NaH 2 PO 4 0.1-0.5%, (NH 4 ) 2 SO 4 0.05-0.2%, MgSO 4 ·7H 2 O 0.01-0.5%, FeCl 2 0.001-0.01%, CaCl 2 0.001-0.01%, yeast extract powder 0.02-0.2%, crude oil 1-10%, polymer (molecular weight 15 million) 0.05-0.1%; pH 6.8-7.5 121°C, sterilization 15-20min. Cultivate for 5-7 days on a shaker at 120 rpm at 45°C.

[0040] Oil plate medium: K 2 HPO 4 0.1-0.5%, KH 2 PO 4 0.1-0.5%, NH 4 NO 3 0.1-0.2%, MgSO 4 ·7H 2 O0.01-0.1%, FeSO 4 0.001-0.01%, CaCl 2 0.001-0.01%, ZnSO 4 0.01-0.02%, CuSO 4 , 0.0005-0.001%, urea 0.01-0.1%, yeast extract powder 0.02-0.2%, crude oil 1-10%, polymer (molecular weight 15 million) 0.05-0.1%, agar 1.5-2%, pH 6.8-7.5, Sterilize at 121°C for 15-20min.

[0041] Slant medium: peptone 1%, beef extract 0.3%, sodium oxide 0.5%, the rest is water, pH 7.2.

[0042] The strains to be screened must not only adapt to...

Embodiment 2

[0048] Example 2. Performance evaluation of Bacillus licheniformis U1-3 CGMCC No. 2437

[0049] The performance of Bacillus licheniformis U1-3CGMCC No. 2437 obtained in Example 1 was evaluated from the following aspects:

[0050] 1. Determination of the surface activity of the fermentation broth

[0051] The oil discharge activity of the biosurfactant produced by Bacillus licheniformis U1-3 CGMCC No. 2437 was determined by the following method: Take a petri dish, add water, and add 0.1-0.2 mL of n-alkanes on the water to form an oil film (Carry out at 45°C), and then add the fermentation broth obtained in step 2 of Example 1 to the center of the oil film. Results The center oil film was squeezed around to form a circle. The diameter of the circle was proportional to the content and activity of the surfactant. The diameter of the circle produced by the strain was measured to be greater than 3cm, indicating that Bacillus licheniformis U1-3 CGMCC No. 2437 fermentation broth has high ...

Embodiment 3

[0058] Example 3. The effect of Bacillus licheniformis U1-3 CGMCC No. 2437 on the alkane composition of crude oil

[0059] To detect the effect of Bacillus licheniformis U1-3 CGMCC No. 2437 on the alkane composition of crude oil, the method is as follows: first take a 250mL Erlenmeyer flask, add 5-10g of oil sample, Bacillus licheniformis U1-3 CGMCC No. 2437 Bacteria liquid 10mL (bacteria content>10 7 cfu / mL) and 150mL of enriched culture solution, shake culture at 45-50℃ and 150rpm for 7-15 days. After the culture is completed, collect the fermentation broth, centrifuge at 3500rpm for 15min to dehydrate, and use the full hydrocarbon chromatography analysis method (The People’s Republic of China) Natural gas industry standard SY / T5779-1995 Crude Oil Total Hydrocarbon Gas Chromatography Analysis Method) The crude oil before and after Bacillus licheniformis U1-3CGMCC No. 2437 is subjected to total hydrocarbon chromatographic analysis (3 parallel detections).

[0060] The result is l...

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Abstract

The invention discloses Bacillus licheniformis U1-3CGMCC No.2437 and application thereof. The microbial inoculum which contains the Bacillus licheniformis U1-3CGMCC No.2437 is also protected by the invention. Model oil displacement test results show that the Bacillus licheniformis after polymer flooding for oil displacement can improve the recovery ratio by 3 to 5 percent (OOIP), the Bacillus licheniformis after polymer flooding for polymer protecting plug oil displacement can improve the recovery ratio by about 7 percent (OOIP), and the Bacillus licheniformis after polymer flooding for oil displacement combined with the chemical flooding can improve the recovery ratio by 6 to 13 percent (OOIP). The test results prove that the Bacillus licheniformis U1-3CGMCC No.2437 can be widely applied in the oil production engineering field, in particular the microorganism intensified oil production field, and is suitable for wide promotion and application.

Description

Technical field [0001] The invention relates to the field of microbial oil recovery, in particular to a strain of Bacillus licheniformis and its application in oil recovery engineering. Background technique [0002] Petroleum is a non-renewable energy source. In order to effectively utilize petroleum resources, countries all over the world are seeking efficient methods to increase oil recovery to extract crude oil that is stuck in the formation and difficult to be extracted by conventional oil extraction methods. Since the 1980s, many countries have carried out research on the technology of using microbial methods to improve oil recovery. After more than 20 years of hard work, the technology has made great progress. [0003] Daqing Oilfield polymer flooding has passed the national "Eighth Five-Year" and "Ninth Five-Year" key scientific and technological research, and has made breakthrough progress. It has entered the industrial application stage from pilot field tests, and the re...

Claims

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Application Information

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IPC IPC(8): C12N1/20E21B43/22C12R1/10
Inventor 石梅侯兆伟李蔚乐建君陈星宏
Owner DAQING OILFIELD CO LTD
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