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Plant desensitizer and preparation method thereof

A technology of plant antisensitizers and antisensitizers, applied in the direction of plant raw materials, plant/algae/fungus/moss components, drug combinations, etc., can solve the problems of high cost, cumbersome operation steps, and difficult removal, and achieve stable quality, The anti-allergic effect is obvious, and the effect of extraction and separation method is simple

Active Publication Date: 2011-06-22
SHANGHAI PECHOIN BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the extraction of effective components of cactus is generally carried out through organic solvent extraction or mild alkali extraction. These methods require the introduction of organic solvents, which are costly and difficult to remove; moreover, isoelectric point precipitation is generally used in the protein separation process. The removal efficiency of miscellaneous proteins is low, and the removal process requires centrifugation, which is not conducive to industrial production; these extraction methods also have the disadvantages of cumbersome operation steps, many reagents are added, and the purity of the obtained products is not high.

Method used

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  • Plant desensitizer and preparation method thereof
  • Plant desensitizer and preparation method thereof
  • Plant desensitizer and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Embodiment 1, the production of plant antisensitizer

[0026] The production process of plant allergens is as follows: figure 1 As shown, the specific process is as follows:

[0027] 1) Mix 100g of cactus powder (purchased from Xinsheng Biotechnology Development Co., Ltd.) and 2L of water at a mass ratio of 1:20, then add 20g of cellulase with an enzyme activity of 1 million U / g, and stir at 50°C for 1h , take no precipitation as an index to obtain the aqueous solution of cactus powder;

[0028] 2) heating the aqueous solution of the above-mentioned cactus powder at 80° C. for 10 minutes to inactivate the enzyme, leave the solution after the enzyme inactivation for 2 hours, and take the supernatant;

[0029] 3) Take 1800ml of the supernatant from the above step 2), adjust the pH value to 4.2, then add 36g of activated carbon to it, so that the ratio of activated carbon and supernatant is 1:50, and stir at 70°C for 1.5 hours;

[0030] 4) Centrifuge the reaction soluti...

Embodiment 2

[0035] Embodiment 2, the functional experiment of plant antisensitizer

[0036] 1. Moisturizing effect experiment

[0037] Select 20 volunteers, half male and half female, wash the left and right forearms of the volunteers with clean water, and conduct the moisturizing effect experiment without applying any cosmetics.

[0038] Use a moisture tester to test the moisture content and TEWL value of the left and right forearm skin of the subject, and then smear the plant antisensitizer prepared in Example 1 on the left forearm of the subject, and use the right forearm as a blank control without applying any cosmetics . Tested every half hour for a total of 4 hours. The experiment was repeated three times, and the curves of the average moisture content and the percentage of average moisture loss over time on the skin surface of the subjects were as follows: figure 2 with image 3 shown. The results show that the plant antisensitizer prepared in Example 1 above has a good long-...

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Abstract

The invention discloses a plant desensitizer and a preparation method thereof. The method comprises the following steps: 1) cellulose is used for treating cactus to degrade cell walls and obtain a cell lysate; 2) killing enzyme is carried out for the cell lysate obtained in Step 1, then the solution is allowed to stand and the upper clear liquid is taken; 3) the pH value of the upper clear liquidobtained in Step 2 is regulated to 4.2, then active carbon is added into the liquid, the mixture is stirred and filtered, filtrate is collected and a cactus extract is obtained; and 4) ion exchange chromatography is carried out to the cactus extract obtained in Step 3 to carry out desalination and decoloring, an effluent liquid is collected and the plant desensitizer is obtained. The preparation method of the plant desensitizer needs no organic reagent, has simple extraction and separation methods, and the obtained desensitizer has stable quality and significant anti-allergy effect.

Description

technical field [0001] The invention relates to a plant antisensitizer and a preparation method thereof. Background technique [0002] Allergic reaction refers to the abnormal reaction of the human body after exposure to antigenic substances. The general manifestations of skin allergies are: dryness, itching, redness, and pigmentation. It is different from the normal immune response, not only does not have a protective effect, on the contrary, it leads to physiological dysfunction and tissue damage due to excessive response. Cactus contains a variety of phytonutrients, 18 kinds of amino acids, and various vitamins and minerals, which can bring precious nutrients and energy to the skin, resist external stimulation to the skin, and effectively eliminate skin irritation caused by external stimulation. Surface redness phenomenon, with anti-allergic and soothing effect. [0003] At present, the extraction of effective components of cactus is generally carried out through organ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61P37/08A61K36/33A61K135/00
Inventor 董银卯何聪芬王昌涛王海涛
Owner SHANGHAI PECHOIN BIOTECH CO LTD