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A kind of breeding method of self-incompatibility two-line hybrid of Brassica napus

A technology of Brassica napus and hybrids, applied in the direction of botany equipment and methods, applications, plant genetic improvement, etc., can solve the problem of not breeding self-incompatible hybrids of Brassica napus, and achieve low seed production costs , Improve breeding efficiency, simple breeding method

Inactive Publication Date: 2011-12-21
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although these methods have been proposed for many years, due to the method of breeding self-incompatibility hybrids of Brassica napus, breeders have not yet bred self-incompatibility in Brassica napus that can be widely promoted in production. bastard

Method used

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  • A kind of breeding method of self-incompatibility two-line hybrid of Brassica napus
  • A kind of breeding method of self-incompatibility two-line hybrid of Brassica napus
  • A kind of breeding method of self-incompatibility two-line hybrid of Brassica napus

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] 1. The selection and breeding of Brassica napus self-incompatibility line, the steps are as follows:

[0026] (1) The self-incompatible line S-1300 of Brassica napus was used as the female parent, and it was crossed with the self-compatible Brassica napus line Huashuang 3 to obtain the first generation hybrid (F 1 )seed.

[0027] (2) F of seeding step (1) 1Seeds, at flowering stage of Brassica napus, on hybrid F 1 The main inflorescence of the plant is bagged and self-crossed, and its compatibility is judged, if F 1 If it shows self-compatibility, it will be bagged and self-crossed, if F 1 If it shows self-incompatibility, it will be self-breeded by peeling off the buds, and F will be obtained. 2 Seed;

[0028] (3) F of planting step (2) 2 Seeds; flowering stage of Brassica napus, for each F 2 The plants were bagged and selfed; the self-incompatible plants were selected, and the buds were manually stripped for selfing; the individual plants were harvested. 2 Pla...

Embodiment 2

[0053] Embodiment 2 (test comparison)

[0054] Specific steps are as follows:

[0055] 1. Use the affinity index method to judge the affinity of Brassica napus:

[0056] Carry out the main branch bagging of the investigated Brassica napus plants at the initial flowering stage, when there are 3-5 flowers blooming on the main branch, knock off the flowers on the main branch, and knock off some small buds on the top of the inflorescence center , Then cover the main branch and two side branches with a sulfuric acid paper bag and fasten them with paper clips. Extract the paper bag upward every other day, and pat the paper bag with your hands during the extraction process to scatter the pollen, which is beneficial to the pollination of the flowers and plays a role in assisting pollination. If there are flowers or flower buds on the side buds protruding out of the bag when extracting the paper bag, it should be knocked out in time to avoid the collected seeds from being impure. Af...

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Abstract

The invention belongs to the technical field of rapeseed breeding, and in particular relates to a method for breeding self-incompatible two-line hybrids of Brassica napus. It is characterized in that the present invention aims at the problem that self-incompatible hybrids of Brassica napus cannot be widely promoted in production because of the shortcomings of existing methods for breeding self-incompatible hybrids of Brassica napus, and proposes a cabbage A method for breeding self-incompatible two-line hybrids of Brassica napus, including breeding and breeding of self-incompatible lines of Brassica napus, breeding and breeding of self-incompatible restorer lines of Brassica napus, Brassica napus Breeding of self-incompatible two-line hybrids of rapeseed and methods for producing seeds thereof. The invention provides an effective method for the breeding of self-incompatible hybrids of Brassica napus, which is beneficial to the large-scale popularization of self-incompatible hybrids of Brassica napus and promotes the utilization of heterosis of Brassica napus.

Description

technical field [0001] The invention belongs to the technical field of Brassica napus breeding, in particular to a method for breeding self-incompatible two-line hybrids of Brassica napus. Background technique [0002] A large number of studies at home and abroad have shown that the hybrids between Brassica napus varieties have strong heterosis. At present, the ways to utilize heterosis in Brassica napus include cytoplasmic male sterility (CMS), nuclear male sterility (GMS), self-incompatibility (SI) and chemical male-killing. Brassica napus nuclear male sterility (GMS) has the advantage of stable sterility, but its fertility is separated. When producing seeds, it is necessary to remove 50% of the fertile plants in the sterile line of Brassica napus at the initial flowering stage. Not only will the yield of seed production be reduced and the cost of seed production will be increased, but also the purity of seed production will be affected and production risk will be increas...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/04A01H1/02
CPCA01H1/02
Inventor 马朝芝傅廷栋高长斌涂金星沈金雄
Owner HUAZHONG AGRI UNIV
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