Insoluble biological activity protein as vitreous body oral medicine for treating ophthalmic diseases
A bioactive, vitreous technology, which is applied in the direction of peptide/protein components, pharmaceutical formulations, medical preparations containing active ingredients, etc., can solve the problems of no drug therapy and achieve the effect of improving stability
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example 1
[0025] Example 1. Preparation of insoluble trypsin hydrogel
[0026] 40 mg of bovine trypsin was dissolved in 1 ml of 0.1 molar borate buffer, pH 8.5. After the buffer was dialyzed overnight, it was cooled to 0°C in an ice-water bath, 70 mg of divalent polyethylene glycol disuccinimidyl ester derivative (molecular weight: 20000) was added under stirring, and stirring was continued until all activated polyethylene glycol was dissolved. . The reaction solution was placed at room temperature to react for 2 hours, and then ammonium hydroxide or glycine was added to terminate the reaction. The formed hydrogel can be broken into small pieces. Polyethylene glycol and trypsin that are not cross-linked can be removed by long-term soaking in large amounts of phosphate saline.
example 2
[0027] Example 2. Degradation of VEGF in the vitreous by insoluble trypsin hydrogel
[0028] Dissolve 5 micrograms of human VEGF165 in 0.5 ml of fresh calf vitreous humor, add 2 mg of the insoluble trypsin hydrogel prepared above, and react with shaking at room temperature for 24 hours. The degradation of VEGF165 is shown in the figure.
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