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Insoluble biological activity protein as vitreous body oral medicine for treating ophthalmic diseases

A bioactive, vitreous technology, which is applied in the direction of peptide/protein components, pharmaceutical formulations, medical preparations containing active ingredients, etc., can solve the problems of no drug therapy and achieve the effect of improving stability

Inactive Publication Date: 2009-05-06
马万超
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is currently no effective drug therapy in the world for these eye diseases caused by collagen fibers in the vitreous

Method used

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  • Insoluble biological activity protein as vitreous body oral medicine for treating ophthalmic diseases

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0025] Example 1. Preparation of insoluble trypsin hydrogel

[0026] 40 mg of bovine trypsin was dissolved in 1 ml of 0.1 molar borate buffer, pH 8.5. After the buffer was dialyzed overnight, it was cooled to 0°C in an ice-water bath, 70 mg of divalent polyethylene glycol disuccinimidyl ester derivative (molecular weight: 20000) was added under stirring, and stirring was continued until all activated polyethylene glycol was dissolved. . The reaction solution was placed at room temperature to react for 2 hours, and then ammonium hydroxide or glycine was added to terminate the reaction. The formed hydrogel can be broken into small pieces. Polyethylene glycol and trypsin that are not cross-linked can be removed by long-term soaking in large amounts of phosphate saline.

example 2

[0027] Example 2. Degradation of VEGF in the vitreous by insoluble trypsin hydrogel

[0028] Dissolve 5 micrograms of human VEGF165 in 0.5 ml of fresh calf vitreous humor, add 2 mg of the insoluble trypsin hydrogel prepared above, and react with shaking at room temperature for 24 hours. The degradation of VEGF165 is shown in the figure.

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Abstract

The invention relates to a method for curing a plurality of ophthalmonogy diseases by degrading, blocking, combining and inhabiting vitreous bodies and / or pathogenic restricted substances in the retinal and using an insoluble biological activity protein as a intravitreal medicament according to the unique structure feature of the vitreous body cavity in eyeballs and the pathogenic functions of a plurality of vitreous bodies and / or the pathogenic restricted substances in the retinal of ophthalmonogy patients in a plurality of the ophthalmonogy diseases. For example, diabetic retinopathy, senile macular degeneration, ophthalmonogy diseases caused by retinal artery or vein occlusion, macular edema, proliferative vitreoretinopathy, intravitreal living cells, vitreous macular traction, macular pucker, macular exudate, fibrin deposition, retinopathy of prematurity, floaters, retinal tearing, retinal detachment, macula hole, epiretinal membrane, retinal internal limiting membrane causing surface tension, collagen fibers causing the traction function to the retinal and the like.

Description

Technical field [0001] Soluble biologically active proteins, such as enzymes, antibodies, soluble receptors, binding proteins and their active fragments, are chemically cross-linked or immobilized to become solid or semi-solid substances insoluble in aqueous solutions. This insoluble biologically active protein still has corresponding biological activity and can be used as intravitreal medicine to treat a variety of ophthalmic diseases. Since the soluble biologically active protein is chemically cross-linked or immobilized, its molecule itself becomes more stable, and the formed insoluble product can exist in the vitreous for a long time. Therefore, the insoluble biologically active protein is safe and effective for intravitreal medication. , The dosage and source of medicine are better than soluble biologically active protein. Background technique [0002] The vitreous body of a normal human eyeball is a transparent gel-like substance that fills most of the space inside the eyeb...

Claims

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Application Information

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IPC IPC(8): A61K38/43A61K38/18A61K38/20A61K38/21
Inventor 马万超杨融冰
Owner 马万超
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