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Method for producing Chinese piliferous mycelium powder

A Chinese Mortierella production method technology, which is applied in the production field of Cordyceps sinensis Trichosporum powder, can solve the problems of easy residue of chemical substances, high pollution probability, long production cycle, etc., and achieve no chemical residue and low pollution probability , The effect of small investment in equipment

Active Publication Date: 2010-12-15
QINGHAI JIUSHI WORM GRASS BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Both of the above two patents use the four-stage submerged fermentation method of Mortierella species in China to expand and reproduce step by step in large-scale industrial fermentation tanks to produce bacterial powder. The disadvantage is that the antibiotic fermentation mode is used, and the chemical substances in the culture medium in the bacterial powder are easy to produce. Residue, complex links, high probability of pollution, large investment in equipment, high risk, long production cycle, and low yield

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Crush 10g of wheat and 10g of soybeans through a 10-mesh sieve and mix them with 80g of water in proportion by weight and put them into a culture container. The culture medium accounts for 1 / 5 of the volume of the culture container. Sterilize for 40 Minutes, add 4g of Mortierella sinensis and culture it at 0°C for 3 days, then the surface of the medium will be covered with white hyphae; then add 40g of liquid medium to the culture container covered with white mycelium 40 g of chicken eggs (whole eggs) were cultured at 14° C. for 3 days in terms of weight percentage of the culture medium; the culture was taken out, dried and pulverized to obtain 70 g of Mortierella sinensis powder.

Embodiment 2

[0024] Crush 50g of rice and pass through a 20-mesh sieve and mix it with 50g of milk in proportion by weight and put it into a culture container. Mortierella sinensis species can be covered with white hyphae on the surface of the culture medium after being cultured at 3°C ​​for 2 days; then add 20g of liquid culture medium to the culture container covered with white mycelia, the culture medium 18.2 g of duck eggs (whole eggs) and 1.8 g of peptone were mixed and prepared by weight percentage, and cultured at 0° C. for 4 days; the culture was taken out, dried, and pulverized to obtain 48 g of Mortierella sinensis powder.

Embodiment 3

[0026] 20g of corn and 6g of broad beans were broken through a 10-mesh sieve and mixed with 74g of protein+sugar aqueous solution (1% sugar and 10% protein aqueous solution) in proportion by weight and then placed into a culture vessel. 3 / 5 of the volume, sterilized at 128°C and 0.15Mpa for 27 minutes, inoculated with 3g of Mortierella sinensis and cultured at 6°C for 3 days, the surface of the medium would be covered with white hyphae; then in Add 30g of liquid culture medium to the culture container covered with white hyphae, and the culture medium is mixed with 27.3g of eggs (whole eggs), 0.27g of milk, 0.27g of potatoes, 0.57g of carrot juice, and 1.59g of peptone It is prepared and cultured at 5°C for 4 days; the culture is taken out, dried and pulverized to obtain 55g of Mortierella sinensis powder.

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PUM

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Abstract

The invention relates to a method for producing microbe, in particular to a method for producing hirsutella sinensis fungal powder of aweto. The method comprises the following steps: according to weight percentage, 10 to 50 percent of cereal, 0 to 10 percent of bean and 50 to 70 percent of water are mixed and put in a culture vessel; a culture medium accounts for one fifth to four fifths of the volume of the culture vessel; a hirsutella sinensis strain accounting for 1 to 4 percent of the weight of the culture medium is inoculated and is cultured for 2 to 5 days at a temperature of between 0 and 14 DEG C; hypha overgrows on the surface of the culture medium; a liquid culture medium accounting for 20 to 40 percent of the weight of the culture medium is replenished on the culture vessel; according to the weight percentage, the culture medium comprising 91 to 100 percent of egg, 0 to 0.9 percent of milk, 0 to 0.9 percent of potato and 0 to 1.9 percent of carrot juice is cultured for 3 to8 days at a temperature of between 0 and 14 DEG C; and finally, a culture substance is taken out, dried and pulverized to obtain the hirsutella sinensis bacterial powder. The method has the advantages of short fermentation period, simple process, no chemical residue, small polluting probability, small equipment investment, low cost, no risk and high yield of between 40 and 50 percent.

Description

technical field [0001] The invention relates to a production method of microorganisms, in particular to a production method of Cordyceps sinensis Mortierella sinensis powder. Background technique [0002] Cordyceps sinensis is a complex of insects and bacteria formed after the larvae of Hepialus armoricamus Oberthur of the genus Lepidoptera are infected with the fungus Mortierella chinensis. Chinese patent CN97110448.4 discloses the fermentation production method of Chinese Cordyceps sinensis fungus, and CN200410101728.X discloses the large-scale fermentation production of Cordyceps sinensis and the processing technology of bacterial powder. Both of the above two patents use the four-stage submerged fermentation method of Mortierella species in China to expand and reproduce step by step in large-scale industrial fermentation tanks to produce bacterial powder. The disadvantage is that the antibiotic fermentation mode is used, and the chemical substances in the culture medium ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12M1/24C12R1/645C12R1/745C12R1/79
Inventor 涂峰张玉业李桂全陈永凤李树志
Owner QINGHAI JIUSHI WORM GRASS BIOTECH